(Circular) (Six-base) MAPSORT of: pFN21AE1368.seq Check: 1806 from: 1 to: 6721 pFN21AE1368 6721 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2185 2500 2681 2991 3381 2185 Size: 315 181 310 390 5525 Fragments arranged by size: 5525 390 315 310 181 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2096 2466 4064 1937 Size: 159 370 1598 4594 Fragments arranged by size: 4594 1598 370 159 Acc65I G'GTAC_C Cuts at: 4042 4042 Size: 6721 AclI AA'CG_TT Cuts at: 1563 4848 5221 1563 Size: 3285 373 3063 Fragments arranged by size: 3285 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2666 3446 4784 6168 6436 1632 Size: 953 81 780 1338 1384 268 1917 Fragments arranged by size: 1917 1384 1338 953 780 268 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6524 AflIII A'CryG_T Cuts at: 1203 5620 6597 1203 Size: 4417 977 1327 Fragments arranged by size: 4417 1327 977 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 5450 5450 Size: 6721 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2792 3082 3701 1184 Size: 1608 290 619 4204 Fragments arranged by size: 4204 1608 619 290 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2210 2242 2423 2455 2888 2909 2920 2941 2210 Size: 32 181 32 433 21 11 21 5990 Fragments arranged by size: 5990 433 181 32 32 21 21 11 AlwNI CAG_nnn'CTG Cuts at: 2209 2659 2670 4464 6036 2209 Size: 450 11 1794 1572 2894 Fragments arranged by size: 2894 1794 1572 450 11 ApaI G_GGCC'C Cuts at: 3193 3659 3193 Size: 466 6255 ApaLI G'TGCA_C Cuts at: 4778 5934 4778 Size: 1156 5565 ApoI r'AATT_y Cuts at: 1493 3101 4030 4256 4408 6517 1493 Size: 1608 929 226 152 2109 1697 Fragments arranged by size: 2109 1697 1608 929 226 152 AseI AT'TA_AT Cuts at: 160 1032 5275 160 Size: 872 4243 1606 Fragments arranged by size: 4243 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 2697 3192 3241 3518 3650 4046 1870 Size: 73 754 495 49 277 132 396 4545 Fragments arranged by size: 4545 754 495 396 277 132 73 49 AvrII C'CTAG_G Cuts at: 3308 3308 Size: 6721 BamHI G'GATC_C Cuts at: 2784 4051 4418 2784 Size: 1267 367 5087 Fragments arranged by size: 5087 1267 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 2693 3338 3866 4042 Size: 325 217 627 475 431 645 528 176 Cuts at: 4042 5497 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 645 627 528 475 431 325 217 176 BanII G_rGCy'C Cuts at: 1391 2327 2748 2823 2880 2949 3193 3230 3387 Size: 936 421 75 57 69 244 37 157 Cuts at: 3387 3659 4040 1391 Size: 272 381 4072 Fragments arranged by size: 4072 936 421 381 272 244 157 75 69 57 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3451 928 Size: 971 1552 4198 Fragments arranged by size: 4198 1552 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4912 4946 1903 Size: 34 50 34 2891 34 3678 Fragments arranged by size: 3678 2891 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4613 5823 4613 Size: 1210 5511 BclI T'GATC_A Cuts at: 1548 2661 1548 Size: 1113 5608 BfrBI ATG'CAT Cuts at: 3353 3353 Size: 6721 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3494 5332 Size: 107 122 71 631 605 337 1485 1838 Cuts at: 5332 136 Size: 1525 Fragments arranged by size: 1838 1525 1485 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6716 6716 Size: 6721 BlpI GC'TnA_GC Cuts at: 2678 3806 4138 2678 Size: 1128 332 5261 Fragments arranged by size: 5261 1128 332 Bme1580I G_kGCm'C Cuts at: 1790 2698 3193 3199 3659 3885 4782 5938 1790 Size: 908 495 6 460 226 897 1156 2573 Fragments arranged by size: 2573 1156 908 897 495 460 226 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2104 3187 3699 Size: 416 53 83 399 24 687 1083 512 Cuts at: 3699 4452 5410 442 Size: 753 958 1753 Fragments arranged by size: 1753 1083 958 753 687 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2732 2845 2876 5381 1239 Size: 1493 113 31 2505 2579 Fragments arranged by size: 2579 2505 1493 113 31 Bpu10I CC'TnA_GC Cuts at: 3482 3482 Size: 6721 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4846 5711 6009 4846 Size: 865 298 5558 Fragments arranged by size: 5558 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3121 4000 5384 882 Size: 617 1622 879 1384 2219 Fragments arranged by size: 2219 1622 1384 879 617 BsaAI yAC'GTr Cuts at: 493 1204 2130 4512 493 Size: 711 926 2382 2702 Fragments arranged by size: 2702 2382 926 711 BsaBI GATnn'nnATC Cuts at: 6715 6715 Size: 6721 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2052 4528 4910 Size: 53 83 186 936 494 25 2476 382 Cuts at: 4910 5545 275 Size: 635 1451 Fragments arranged by size: 2476 1451 936 635 494 382 186 83 53 25 BsaWI w'CCGG_w Cuts at: 5154 5552 5826 5973 6384 5154 Size: 398 274 147 411 5491 Fragments arranged by size: 5491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 2395 2423 2425 Size: 30 165 30 906 30 56 28 2 Cuts at: 2425 2453 2909 2939 1178 Size: 28 456 30 4960 Fragments arranged by size: 4960 906 456 165 56 30 30 30 30 28 28 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2065 2930 2933 2936 1186 Size: 879 865 3 3 4971 Fragments arranged by size: 4971 879 865 3 3 BseYI C'CCAG_C Cuts at: 2212 2301 2358 2610 2704 2886 3089 3229 3413 Size: 89 57 252 94 182 203 140 184 Cuts at: 3413 3973 5924 6420 2212 Size: 560 1951 496 2513 Fragments arranged by size: 2513 1951 560 496 252 203 184 182 140 94 89 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3149 3254 3575 1163 Size: 573 1413 105 321 4309 Fragments arranged by size: 4309 1413 573 321 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4171 4932 5960 1540 Size: 275 186 1360 810 761 1028 2301 Fragments arranged by size: 2301 1360 1028 810 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2496 2619 2679 2748 2880 2949 3387 4782 4867 Size: 123 60 69 132 69 438 1395 85 Cuts at: 4867 5938 2496 Size: 1071 3279 Fragments arranged by size: 3279 1395 1071 438 132 123 85 69 69 60 BsmI GAATG_Cn' Cuts at: 4232 4325 4232 Size: 93 6628 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2327 2496 2619 2679 2698 2748 2823 Size: 399 537 169 123 60 19 50 75 Cuts at: 2823 2880 2949 3193 3199 3230 3387 3659 3885 Size: 57 69 244 6 31 157 272 226 Cuts at: 3885 4040 4782 4867 5938 1391 Size: 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 537 399 272 244 226 169 157 155 123 85 75 69 60 57 50 31 19 6 BspEI T'CCGG_A Cuts at: 6384 6384 Size: 6721 BspHI T'CATG_A Cuts at: 4610 6340 4610 Size: 1730 4991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2185 2500 2681 2745 2991 3381 3903 4076 Size: 1341 315 181 64 246 390 522 173 Cuts at: 4076 844 Size: 3489 Fragments arranged by size: 3489 1341 522 390 315 246 181 173 64 BsrBI CCG'CTC Cuts at: 1394 1823 4608 1394 Size: 429 2785 3507 Fragments arranged by size: 3507 2785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3138 5216 5390 65 Size: 1183 1890 2078 174 1396 Fragments arranged by size: 2078 1890 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 2537 2802 2975 3699 3843 5365 Size: 110 219 508 265 173 724 144 1522 Cuts at: 5365 5552 1700 Size: 187 2869 Fragments arranged by size: 2869 1522 724 508 265 219 187 173 144 110 BsrGI T'GTAC_A Cuts at: 96 3135 96 Size: 3039 3682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6721 BssSI C'ACGA_G Cuts at: 4781 5793 4781 Size: 1012 5709 BstAPI GCAn_nnn'nTGC Cuts at: 794 2209 3345 794 Size: 1415 1136 4170 Fragments arranged by size: 4170 1415 1136 BstBI TT'CG_AA Cuts at: 5525 5525 Size: 6721 BstEII G'GTnAC_C Cuts at: 1184 2368 1184 Size: 1184 5537 BstXI CCAn_nnnn'nTGG Cuts at: 2610 2610 Size: 6721 BstYI r'GATC_y Cuts at: 1972 2784 3155 4051 4418 4803 4820 6272 6358 Size: 812 371 896 367 385 17 1452 86 Cuts at: 6358 6370 6716 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 896 812 385 371 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2930 2930 Size: 6721 BtgI C'CryG_G Cuts at: 513 1065 2067 2183 2479 3330 513 Size: 552 1002 116 296 851 3904 Fragments arranged by size: 3904 1002 851 552 296 116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3806 3913 487 Size: 683 56 2580 107 3295 Fragments arranged by size: 3295 2580 683 107 56 BtsI GCAGTG_nn' Cuts at: 3504 3694 3782 4014 4237 5031 5051 3504 Size: 190 88 232 223 794 20 5174 Fragments arranged by size: 5174 794 232 223 190 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3359 3394 529 Size: 35 2795 35 3856 Fragments arranged by size: 3856 2795 35 35 DraI TTT'AAA Cuts at: 4025 4025 Size: 6721 DraIII CAC_nnn'GTG Cuts at: 3345 3345 Size: 6721 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3365 5728 809 Size: 507 2049 2363 1802 Fragments arranged by size: 2363 2049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2977 3501 3580 Size: 54 1425 50 275 18 1147 524 79 Cuts at: 3580 4168 5057 6686 8 Size: 588 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 588 524 275 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4168 1537 Size: 275 2356 4090 Fragments arranged by size: 4090 2356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2249 4651 1353 Size: 734 162 2402 3423 Fragments arranged by size: 3423 2402 734 162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2531 3188 5308 5682 5828 387 Size: 1064 138 942 657 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 942 657 374 146 138 EcoICRI GAG'CTC Cuts at: 2746 2878 2947 3385 2746 Size: 132 69 438 6082 Fragments arranged by size: 6082 438 132 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 2666 2732 2845 2876 3446 4784 Size: 393 953 81 66 113 31 570 1338 Cuts at: 4784 5381 6168 6436 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1338 953 787 597 570 393 268 113 81 66 31 EcoNI CCTnn'n_nnAGG Cuts at: 2273 3278 3989 2273 Size: 1005 711 5005 Fragments arranged by size: 5005 1005 711 EcoO109I rG'GnC_Cy Cuts at: 2484 3036 3313 3655 3656 3748 3912 2484 Size: 552 277 342 1 92 164 5293 Fragments arranged by size: 5293 552 342 277 164 92 1 EcoRI G'AATT_C Cuts at: 4030 4408 4030 Size: 378 6343 FseI GG_CCGG'CC Cuts at: 2979 2979 Size: 6721 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 3029 3489 5868 1678 Size: 1351 460 2379 2531 Fragments arranged by size: 2531 2379 1351 460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2867 2899 3935 3967 5362 Size: 32 135 32 1295 32 1036 32 1395 Cuts at: 5362 5394 5436 5468 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1395 1295 1036 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4065 4311 669 Size: 988 281 2127 246 3079 Fragments arranged by size: 3079 2127 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6721 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KpnI G_GTAC'C Cuts at: 4046 4046 Size: 6721 MfeI C'AATT_G Cuts at: 4320 4320 Size: 6721 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5835 6019 1305 Size: 4530 184 2007 Fragments arranged by size: 4530 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3503 10 Size: 54 1425 343 1671 3228 Fragments arranged by size: 3228 1671 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4007 4679 5038 5197 518 Size: 648 2841 672 359 159 2042 Fragments arranged by size: 2841 2042 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2104 2421 2610 2635 2667 2864 3081 Size: 463 35 317 189 25 32 197 217 Cuts at: 3081 3669 3802 4137 4814 5962 6207 1606 Size: 588 133 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 588 463 335 317 245 217 197 189 133 35 32 25 NaeI GCC'GGC Cuts at: 2031 2804 2977 3701 3845 2031 Size: 773 173 724 144 4907 Fragments arranged by size: 4907 773 724 173 144 NcoI C'CATG_G Cuts at: 513 1065 2479 513 Size: 552 1414 4755 Fragments arranged by size: 4755 1414 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6721 NgoMIV G'CCGG_C Cuts at: 2029 2802 2975 3699 3843 2029 Size: 773 173 724 144 4907 Fragments arranged by size: 4907 773 724 173 144 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6721 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NsiI A_TGCA'T Cuts at: 3355 3355 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 4079 5624 1132 Size: 97 2850 1545 2229 Fragments arranged by size: 2850 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2796 1348 Size: 1448 5273 PciI A'CATG_T Cuts at: 5620 5620 Size: 6721 PflMI CCAn_nnn'nTGG Cuts at: 1108 3105 3262 3758 1108 Size: 1997 157 496 4071 Fragments arranged by size: 4071 1997 496 157 PfoI T'CCnGG_A Cuts at: 2046 2268 2287 2892 2046 Size: 222 19 605 5875 Fragments arranged by size: 5875 605 222 19 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PmlI CAC'GTG Cuts at: 2130 2130 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2423 2455 2909 2941 3596 3628 4790 4822 6303 Size: 32 454 32 655 32 1162 32 1481 Cuts at: 6303 6335 2423 Size: 32 2809 Fragments arranged by size: 2809 1481 1162 655 454 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2484 3313 3748 2484 Size: 829 435 5457 Fragments arranged by size: 5457 829 435 PshAI GACnn'nnGTC Cuts at: 1613 2655 1613 Size: 1042 5679 PsiI TTA'TAA Cuts at: 4291 4291 Size: 6721 PspOMI G'GGCC_C Cuts at: 3189 3655 3189 Size: 466 6255 PstI C_TGCA'G Cuts at: 830 2253 2988 3153 3795 3865 4073 830 Size: 1423 735 165 642 70 208 3478 Fragments arranged by size: 3478 1423 735 642 208 165 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 PvuII CAG'CTG Cuts at: 2635 2667 2635 Size: 32 6689 SacI G_AGCT'C Cuts at: 2748 2880 2949 3387 2748 Size: 132 69 438 6082 Fragments arranged by size: 6082 438 132 69 SacII CC_GC'GG Cuts at: 2070 2070 Size: 6721 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SapI GCTCTTCn'nnn_ Cuts at: 2087 2249 2087 Size: 162 6559 SbfI CC_TGCA'GG Cuts at: 3865 4073 3865 Size: 208 6513 ScaI AGT'ACT Cuts at: 4969 6681 4969 Size: 1712 5009 SexAI A'CCwGG_T Cuts at: 2274 2274 Size: 6721 SfcI C'TryA_G Cuts at: 826 1045 2249 2984 3149 3791 3861 4069 5204 Size: 219 1204 735 165 642 70 208 1135 Cuts at: 5204 5885 6076 826 Size: 681 191 1471 Fragments arranged by size: 1471 1204 1135 735 681 642 219 208 191 165 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 2029 1700 Size: 329 6392 SmaI CCC'GGG Cuts at: 1872 2699 3194 3243 3520 4048 1872 Size: 827 495 49 277 528 4545 Fragments arranged by size: 4545 827 528 495 277 49 SmlI C'TyrA_G Cuts at: 820 1017 1943 4825 5726 5988 820 Size: 197 926 2882 901 262 1553 Fragments arranged by size: 2882 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6721 SphI G_CATG'C Cuts at: 1132 4079 1132 Size: 2947 3774 SrfI GCCC'GGGC Cuts at: 3194 3194 Size: 6721 SspI AAT'ATT Cuts at: 5 52 4645 5 Size: 47 4593 2081 Fragments arranged by size: 4593 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6721 StyI C'CwwG_G Cuts at: 513 1065 2472 2479 3308 4160 513 Size: 552 1407 7 829 852 3074 Fragments arranged by size: 3074 1407 852 829 552 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2350 2899 3194 3634 4748 4765 4918 Size: 1255 770 549 295 440 1114 17 153 Cuts at: 4918 5103 5548 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1114 770 549 445 440 295 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3135 4967 6679 Size: 274 80 33 51 1443 1158 1832 1712 Cuts at: 6679 96 Size: 138 Fragments arranged by size: 1832 1712 1443 1158 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2114 2146 2346 2378 6681 6713 2114 Size: 32 200 32 4303 32 2122 Fragments arranged by size: 4303 2122 200 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2276 1316 Size: 960 5761 XbaI T'CTAG_A Cuts at: 4057 4057 Size: 6721 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3328 3496 3328 Size: 168 6553 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 2697 3192 3241 3518 4046 1870 Size: 827 495 49 277 528 4545 Fragments arranged by size: 4545 827 528 495 277 49 XmnI GAAnn'nnTTC Cuts at: 4850 4850 Size: 6721 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I EcoRV FalI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SanDI SfiI SfoI SwaI