(Linear) (Six-base) MAPSORT of: KIEE1368.seq Check: 5120 from: 1 to: 2016 KIEE1368.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 180 495 676 986 1376 2016 Size: 180 315 181 310 390 640 Fragments arranged by size: 640 390 315 310 181 180 AccI GT'mk_AC Cuts at: 0 91 461 2016 Size: 91 370 1555 Fragments arranged by size: 1555 370 91 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 661 1441 2016 Size: 580 81 780 575 Fragments arranged by size: 780 580 575 81 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 787 1077 1696 2016 Size: 787 290 619 320 Fragments arranged by size: 787 619 320 290 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 205 237 418 450 883 904 915 936 Size: 205 32 181 32 433 21 11 21 Cuts at: 936 2016 Size: 1080 Fragments arranged by size: 1080 433 205 181 32 32 21 21 11 AlwNI CAG_nnn'CTG Cuts at: 0 204 654 665 2016 Size: 204 450 11 1351 Fragments arranged by size: 1351 450 204 11 ApaI G_GGCC'C Cuts at: 0 1188 1654 2016 Size: 1188 466 362 Fragments arranged by size: 1188 466 362 ApoI r'AATT_y Cuts at: 0 1096 2016 Size: 1096 920 AvaI C'yCGr_G Cuts at: 0 692 1187 1236 1513 1645 2016 Size: 692 495 49 277 132 371 Fragments arranged by size: 692 495 371 277 132 49 AvrII C'CTAG_G Cuts at: 0 1303 2016 Size: 1303 713 BamHI G'GATC_C Cuts at: 0 779 2016 Size: 779 1237 BanI G'GyrC_C Cuts at: 0 257 688 1333 1861 2016 Size: 257 431 645 528 155 Fragments arranged by size: 645 528 431 257 155 BanII G_rGCy'C Cuts at: 0 322 743 818 875 944 1188 1225 1382 Size: 322 421 75 57 69 244 37 157 Cuts at: 1382 1654 2016 Size: 272 362 Fragments arranged by size: 421 362 322 272 244 157 75 69 57 37 BbsI GAAGACnn'nnnn_ Cuts at: 0 1446 2016 Size: 1446 570 BclI T'GATC_A Cuts at: 0 656 2016 Size: 656 1360 BfrBI ATG'CAT Cuts at: 0 1348 2016 Size: 1348 668 BglI GCCn_nnn'nGGC Cuts at: 0 1489 2016 Size: 1489 527 BlpI GC'TnA_GC Cuts at: 0 673 1801 2016 Size: 673 1128 215 Fragments arranged by size: 1128 673 215 Bme1580I G_kGCm'C Cuts at: 0 693 1188 1194 1654 1880 2016 Size: 693 495 6 460 226 136 Fragments arranged by size: 693 495 460 226 136 6 BmrI ACTGGGnnnn_n' Cuts at: 0 99 1182 1694 2016 Size: 99 1083 512 322 Fragments arranged by size: 1083 512 322 99 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 727 840 871 2016 Size: 727 113 31 1145 Fragments arranged by size: 1145 727 113 31 Bpu10I CC'TnA_GC Cuts at: 0 1477 2016 Size: 1477 539 BsaI GGTCTCn'nnnn_ Cuts at: 0 1116 1995 2016 Size: 1116 879 21 Fragments arranged by size: 1116 879 21 BsaAI yAC'GTr Cuts at: 0 125 2016 Size: 125 1891 BsaHI Gr'CG_yC Cuts at: 0 22 47 2016 Size: 22 25 1969 Fragments arranged by size: 1969 25 22 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 304 334 390 418 420 448 904 934 Size: 304 30 56 28 2 28 456 30 Cuts at: 934 2016 Size: 1082 Fragments arranged by size: 1082 456 304 56 30 30 28 28 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 60 925 928 931 2016 Size: 60 865 3 3 1085 Fragments arranged by size: 1085 865 60 3 3 BseYI C'CCAG_C Cuts at: 0 207 296 353 605 699 881 1084 1224 Size: 207 89 57 252 94 182 203 140 Cuts at: 1224 1408 1968 2016 Size: 184 560 48 Fragments arranged by size: 560 252 207 203 184 182 140 94 89 57 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1144 1249 1570 2016 Size: 1144 105 321 446 Fragments arranged by size: 1144 446 321 105 BsiEI CG_ry'CG Cuts at: 0 1356 2016 Size: 1356 660 BsiHKAI G_wGCw'C Cuts at: 0 491 614 674 743 875 944 1382 2016 Size: 491 123 60 69 132 69 438 634 Fragments arranged by size: 634 491 438 132 123 69 69 60 Bsp1286I G_dGCh'C Cuts at: 0 322 491 614 674 693 743 818 875 Size: 322 169 123 60 19 50 75 57 Cuts at: 875 944 1188 1194 1225 1382 1654 1880 2016 Size: 69 244 6 31 157 272 226 136 Fragments arranged by size: 322 272 244 226 169 157 136 123 75 69 60 57 50 31 19 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 180 495 676 740 986 1376 1898 2016 Size: 180 315 181 64 246 390 522 118 Fragments arranged by size: 522 390 315 246 181 180 118 64 BsrDI GCAATG_nn' Cuts at: 0 1133 2016 Size: 1133 883 BsrFI r'CCGG_y Cuts at: 0 24 532 797 970 1694 1838 2016 Size: 24 508 265 173 724 144 178 Fragments arranged by size: 724 508 265 178 173 144 24 BsrGI T'GTAC_A Cuts at: 0 1130 2016 Size: 1130 886 BstAPI GCAn_nnn'nTGC Cuts at: 0 204 1340 2016 Size: 204 1136 676 Fragments arranged by size: 1136 676 204 BstEII G'GTnAC_C Cuts at: 0 363 2016 Size: 363 1653 BstXI CCAn_nnnn'nTGG Cuts at: 0 605 2016 Size: 605 1411 BstYI r'GATC_y Cuts at: 0 779 1150 2016 Size: 779 371 866 Fragments arranged by size: 866 779 371 Bsu36I CC'TnA_GG Cuts at: 0 925 2016 Size: 925 1091 BtgI C'CryG_G Cuts at: 0 62 178 474 1325 2016 Size: 62 116 296 851 691 Fragments arranged by size: 851 691 296 116 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1801 1908 2016 Size: 1801 107 108 Fragments arranged by size: 1801 108 107 BtsI GCAGTG_nn' Cuts at: 0 1499 1689 1777 2009 2016 Size: 1499 190 88 232 7 Fragments arranged by size: 1499 232 190 88 7 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1354 1389 2016 Size: 1354 35 627 Fragments arranged by size: 1354 627 35 DraIII CAC_nnn'GTG Cuts at: 0 1340 2016 Size: 1340 676 DrdI GACnn_nn'nnGTC Cuts at: 0 1360 2016 Size: 1360 656 EaeI y'GGCC_r Cuts at: 0 972 1496 1575 2016 Size: 972 524 79 441 Fragments arranged by size: 972 524 441 79 EarI CTCTTCn'nnn_ Cuts at: 0 82 244 2016 Size: 82 162 1772 Fragments arranged by size: 1772 162 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 526 1183 2016 Size: 526 657 833 Fragments arranged by size: 833 657 526 EcoICRI GAG'CTC Cuts at: 0 741 873 942 1380 2016 Size: 741 132 69 438 636 Fragments arranged by size: 741 636 438 132 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 661 727 840 871 1441 2016 Size: 580 81 66 113 31 570 575 Fragments arranged by size: 580 575 570 113 81 66 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 268 1273 1984 2016 Size: 268 1005 711 32 Fragments arranged by size: 1005 711 268 32 EcoO109I rG'GnC_Cy Cuts at: 0 479 1031 1308 1650 1651 1743 1907 2016 Size: 479 552 277 342 1 92 164 109 Fragments arranged by size: 552 479 342 277 164 109 92 1 FseI GG_CCGG'CC Cuts at: 0 974 2016 Size: 974 1042 HaeII r_GCGC'y Cuts at: 0 1024 1484 2016 Size: 1024 460 532 Fragments arranged by size: 1024 532 460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 862 894 1930 1962 2016 Size: 862 32 1036 32 54 Fragments arranged by size: 1036 862 54 32 32 MscI TGG'CCA Cuts at: 0 1498 2016 Size: 1498 518 MslI CAynn'nnrTG Cuts at: 0 2002 2016 Size: 2002 14 MspA1I CmG'CkG Cuts at: 0 64 99 416 605 630 662 859 1076 Size: 64 35 317 189 25 32 197 217 Cuts at: 1076 1664 1797 2016 Size: 588 133 219 Fragments arranged by size: 588 317 219 217 197 189 133 64 35 32 25 NaeI GCC'GGC Cuts at: 0 26 799 972 1696 1840 2016 Size: 26 773 173 724 144 176 Fragments arranged by size: 773 724 176 173 144 26 NcoI C'CATG_G Cuts at: 0 474 2016 Size: 474 1542 NgoMIV G'CCGG_C Cuts at: 0 24 797 970 1694 1838 2016 Size: 24 773 173 724 144 178 Fragments arranged by size: 773 724 178 173 144 24 NsiI A_TGCA'T Cuts at: 0 1350 2016 Size: 1350 666 PasI CC'CwG_GG Cuts at: 0 791 2016 Size: 791 1225 PflMI CCAn_nnn'nTGG Cuts at: 0 1100 1257 1753 2016 Size: 1100 157 496 263 Fragments arranged by size: 1100 496 263 157 PfoI T'CCnGG_A Cuts at: 0 41 263 282 887 2016 Size: 41 222 19 605 1129 Fragments arranged by size: 1129 605 222 41 19 PmlI CAC'GTG Cuts at: 0 125 2016 Size: 125 1891 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 418 450 904 936 1591 1623 2016 Size: 418 32 454 32 655 32 393 Fragments arranged by size: 655 454 418 393 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 479 1308 1743 2016 Size: 479 829 435 273 Fragments arranged by size: 829 479 435 273 PshAI GACnn'nnGTC Cuts at: 0 650 2016 Size: 650 1366 PspOMI G'GGCC_C Cuts at: 0 1184 1650 2016 Size: 1184 466 366 Fragments arranged by size: 1184 466 366 PstI C_TGCA'G Cuts at: 0 248 983 1148 1790 1860 2016 Size: 248 735 165 642 70 156 Fragments arranged by size: 735 642 248 165 156 70 PvuII CAG'CTG Cuts at: 0 630 662 2016 Size: 630 32 1354 Fragments arranged by size: 1354 630 32 SacI G_AGCT'C Cuts at: 0 743 875 944 1382 2016 Size: 743 132 69 438 634 Fragments arranged by size: 743 634 438 132 69 SacII CC_GC'GG Cuts at: 0 65 2016 Size: 65 1951 SapI GCTCTTCn'nnn_ Cuts at: 0 82 244 2016 Size: 82 162 1772 Fragments arranged by size: 1772 162 82 SbfI CC_TGCA'GG Cuts at: 0 1860 2016 Size: 1860 156 SexAI A'CCwGG_T Cuts at: 0 269 2016 Size: 269 1747 SfcI C'TryA_G Cuts at: 0 244 979 1144 1786 1856 2016 Size: 244 735 165 642 70 160 Fragments arranged by size: 735 642 244 165 160 70 SgrAI Cr'CCGG_yG Cuts at: 0 24 2016 Size: 24 1992 SmaI CCC'GGG Cuts at: 0 694 1189 1238 1515 2016 Size: 694 495 49 277 501 Fragments arranged by size: 694 501 495 277 49 SrfI GCCC'GGGC Cuts at: 0 1189 2016 Size: 1189 827 StyI C'CwwG_G Cuts at: 0 467 474 1303 2016 Size: 467 7 829 713 Fragments arranged by size: 829 713 467 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 345 894 1189 1629 2016 Size: 345 549 295 440 387 Fragments arranged by size: 549 440 387 345 295 TatI w'GTAC_w Cuts at: 0 1130 2016 Size: 1130 886 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 109 141 341 373 2016 Size: 109 32 200 32 1643 Fragments arranged by size: 1643 200 109 32 32 Tth111I GACn'n_nGTC Cuts at: 0 271 2016 Size: 271 1745 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1323 1491 2016 Size: 1323 168 525 Fragments arranged by size: 1323 525 168 XmaI C'CCGG_G Cuts at: 0 692 1187 1236 1513 2016 Size: 692 495 49 277 503 Fragments arranged by size: 692 503 495 277 49 Enzymes that do cut: AarI AccI AcuI AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbsI BclI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NsiI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SgrAI SmaI SrfI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI BcgI BciVI BglII BmgBI BmtI BplI BpuEI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstZ17I ClaI DraI EagI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NspI PacI PciI PmeI PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI SfiI SfoI SgfI SmlI SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI XmnI ZraI