################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AviII AvrII BbeI BbrPI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspEI BsrDI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FseI FspI HaeII HhaI HinP1I HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1111 GACGTC GACGT^C AccI 1933 GTCTAC GT^[AC][GT]AC AcyI 1111 GACGTC G[GA]^CG[CT]C AseI 1681 ATTAAT AT^TAAT AsnI 1681 ATTAAT AT^TAAT AspHI 246 GAGCAC G[AT]GC[AT]^C BamHI 103 GGATCC G^GATCC BanII 414 GGGCTC G[GA]GC[CT]^C BbvCI 739 GCTGAGG CCTCAGC(-5/-2) BclI 1736 TGATCA T^GATCA BglI 1072 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1111 GACGTC G[GA]^CG[CT]C BsiHKAI 246 GAGCAC G[AT]GC[AT]^C Bsp1407I 1085 TGTACA T^GTACA BspDI 1405 ATCGAT AT^CGAT BspHI 1157 TCATGA T^CATGA BspLU11I 1350 ACATGT A^CATGT BsrFI 446 GCCGGT [GA]^CCGG[CT] BsrGI 1085 TGTACA T^GTACA BssSI 1522 CACGAG CACGAG(-5/-1) BstEII 2025 GGTCACC G^GT[ACGT]ACC BstPI 2025 GGTCACC G^GT[ACGT]ACC BstXI 435 CCAAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 446 GCCGGT [GA]^CCGG[CT] ClaI 1405 ATCGAT AT^CGAT EarI 1858 GAAGAG CTCTTC(1/4) EcoNI 1117 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2025 GGTCACC G^GT[ACGT]ACC EcoRI 1703 GAATTC G^AATTC EcoRV 1879 GATATC GAT^ATC FbaI 1736 TGATCA T^GATCA HgaI 557 GCGTC GACGC(5/10) Hin1I 1111 GACGTC G[GA]^CG[CT]C HincII 880 GTCAAC GT[CT]^[GA]AC HindII 880 GTCAAC GT[CT]^[GA]AC HindIII 695 AAGCTT A^AGCTT Ksp632I 1858 GAAGAG CTCTTC(1/4) NdeI 938 CATATG CA^TATG NspI 1350 ACATGT [GA]CATG^[CT] PaeR7I 1811 CTCGAG C^TCGAG PshBI 1681 ATTAAT AT^TAAT RcaI 1157 TCATGA T^CATGA SapI 1858 GAAGAGC GCTCTTC(1/4) ScaI 1553 AGTACT AGT^ACT SexAI 815 ACCTGGT A^CC[AT]GGT SmaI 501 CCCGGG CCC^GGG SspBI 1085 TGTACA T^GTACA TfiI 2092 GATTC G^A[AT]TC XhoI 1811 CTCGAG C^TCGAG XmaCI 501 CCCGGG C^CCGGG XmaI 501 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Acc65I 1459 GGTACC G^GTACC 2199 GGTACC AlwNI 166 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 1417 CAGTCTCTG Asp718I 1459 GGTACC G^GTACC 2199 GGTACC BalI 1102 TGGCCA TGG^CCA 1764 TGGCCA BanI 1459 GGTACC G^G[CT][GA]CC 2199 GGTACC BsaAI 178 TACGTG [CT]AC^GT[GA] 218 CACGTA BsaI 718 GAGACC GGTCTC(1/5) 350 GGTCTC BsrBI 482 GAGCGG CCGCTC(-3/-3) 633 GAGCGG DsaI 79 CCATGG C^C[GA][CT]GG 1480 CCATGG KpnI 1459 GGTACC GGTAC^C 2199 GGTACC MluNI 1102 TGGCCA TGG^CCA 1764 TGGCCA MscI 1102 TGGCCA TGG^CCA 1764 TGGCCA MseI 886 TTAA T^TAA 1682 TTAA MslI 388 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1152 CATCCTCATG NcoI 79 CCATGG C^CATGG 1480 CCATGG PstI 225 CTGCAG CTGCA^G 570 CTGCAG PvuII 1962 CAGCTG CAG^CTG 2151 CAGCTG SfcI 225 CTGCAG C^T[GA][CT]AG 570 CTGCAG Tru9I 886 TTAA T^TAA 1682 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AspI AvaI AvaII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII