(Circular) (Six-base) MAPSORT of: pFN21AE1431.seq Check: 758 from: 1 to: 6997 pFN21AE1431 6997 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2444 2444 Size: 6997 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3120 4807 278 Size: 53 83 186 936 1584 1687 2468 Fragments arranged by size: 2468 1687 1584 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3939 4340 1937 Size: 2002 401 4594 Fragments arranged by size: 4594 2002 401 Acc65I G'GTAC_C Cuts at: 3464 4204 4318 3464 Size: 740 114 6143 Fragments arranged by size: 6143 740 114 AclI AA'CG_TT Cuts at: 1563 5124 5497 1563 Size: 3561 373 3063 Fragments arranged by size: 3561 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 2784 3000 3147 4280 5060 6444 6712 Size: 647 505 216 147 1133 780 1384 268 Cuts at: 6712 1632 Size: 1917 Fragments arranged by size: 1917 1384 1133 780 647 505 268 216 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6800 AflIII A'CryG_T Cuts at: 1203 2184 2222 2615 3355 5896 6873 1203 Size: 981 38 393 740 2541 977 1327 Fragments arranged by size: 2541 1327 981 977 740 393 38 AgeI A'CCGG_T Cuts at: 5828 5828 Size: 6997 AhdI GACnn_n'nnGTC Cuts at: 5726 5726 Size: 6997 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6997 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4224 1184 Size: 3040 3957 AlwNI CAG_nnn'CTG Cuts at: 2176 3427 4740 6312 2176 Size: 1251 1313 1572 2861 Fragments arranged by size: 2861 1572 1313 1251 ApaLI G'TGCA_C Cuts at: 5054 6210 5054 Size: 1156 5841 ApoI r'AATT_y Cuts at: 1493 2961 3708 3716 4306 4532 4684 6793 1493 Size: 1468 747 8 590 226 152 2109 1697 Fragments arranged by size: 2109 1697 1468 747 590 226 152 8 AseI AT'TA_AT Cuts at: 160 1032 3687 5551 160 Size: 872 2655 1864 1606 Fragments arranged by size: 2655 1864 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 AvaI C'yCGr_G Cuts at: 1870 1943 2506 3816 4218 4322 1870 Size: 73 563 1310 402 104 4545 Fragments arranged by size: 4545 1310 563 402 104 73 BamHI G'GATC_C Cuts at: 2108 4327 4694 2108 Size: 2219 367 4411 Fragments arranged by size: 4411 2219 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3464 4204 4318 5773 618 Size: 325 217 627 1677 740 114 1455 1842 Fragments arranged by size: 1842 1677 1455 740 627 325 217 114 BanII G_rGCy'C Cuts at: 1391 2423 4316 1391 Size: 1032 1893 4072 Fragments arranged by size: 4072 1893 1032 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2267 2562 2703 3045 928 Size: 971 368 295 141 342 4880 Fragments arranged by size: 4880 971 368 342 295 141 BbvCI CC'TCA_GC Cuts at: 2745 2745 Size: 6997 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5188 5222 1903 Size: 34 3251 34 3678 Fragments arranged by size: 3678 3251 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4889 6099 4889 Size: 1210 5787 BclI T'GATC_A Cuts at: 1548 3741 1548 Size: 2193 4804 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3083 5608 136 Size: 107 122 71 631 605 1411 2525 1525 Fragments arranged by size: 2525 1525 1411 631 605 122 107 71 BglII A'GATC_T Cuts at: 2234 2382 3498 6992 2234 Size: 148 1116 3494 2239 Fragments arranged by size: 3494 2239 1116 148 BlpI GC'TnA_GC Cuts at: 4414 4414 Size: 6997 Bme1580I G_kGCm'C Cuts at: 1790 2757 5058 6214 1790 Size: 967 2301 1156 2573 Fragments arranged by size: 2573 2301 1156 967 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6997 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3013 4728 5686 Size: 416 53 83 399 24 1596 1715 958 Cuts at: 5686 442 Size: 1753 Fragments arranged by size: 1753 1715 1596 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6997 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3849 3999 3849 Size: 150 6847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 2141 2198 2269 3065 3668 4074 5657 Size: 846 56 57 71 796 603 406 1583 Cuts at: 5657 1239 Size: 2579 Fragments arranged by size: 2579 1583 846 796 603 406 71 57 56 Bpu10I CC'TnA_GC Cuts at: 2745 2745 Size: 6997 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2250 2670 3734 5122 5987 6285 2250 Size: 420 1064 1388 865 298 2962 Fragments arranged by size: 2962 1388 1064 865 420 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2361 2717 5660 882 Size: 617 862 356 2943 2219 Fragments arranged by size: 2943 2219 862 617 356 BsaAI yAC'GTr Cuts at: 493 1204 2185 2225 4788 493 Size: 711 981 40 2563 2702 Fragments arranged by size: 2702 2563 981 711 40 BsaBI GATnn'nnATC Cuts at: 6991 6991 Size: 6997 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3117 4804 5186 5821 Size: 53 83 186 936 1584 1687 382 635 Cuts at: 5821 275 Size: 1451 Fragments arranged by size: 1687 1584 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5430 5828 6102 6249 6660 5430 Size: 398 274 147 411 5767 Fragments arranged by size: 5767 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2065 2095 2171 2201 1178 Size: 30 165 30 662 30 76 30 5974 Fragments arranged by size: 5974 662 165 76 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3923 3926 4071 1186 Size: 2737 3 145 4112 Fragments arranged by size: 4112 2737 145 3 BseYI C'CCAG_C Cuts at: 2604 4158 6200 6696 2604 Size: 1554 2042 496 2905 Fragments arranged by size: 2905 2042 1554 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2317 3784 3861 1163 Size: 573 501 80 1467 77 4299 Fragments arranged by size: 4299 1467 573 501 80 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4447 5208 6236 1540 Size: 275 186 2446 761 1028 2301 Fragments arranged by size: 2446 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2255 5058 5143 6214 2255 Size: 2803 85 1071 3038 Fragments arranged by size: 3038 2803 1071 85 BsmI GAATG_Cn' Cuts at: 4508 4601 4508 Size: 93 6904 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2423 2757 4316 5058 5143 6214 Size: 399 465 168 334 1559 742 85 1071 Cuts at: 6214 1391 Size: 2174 Fragments arranged by size: 2174 1559 1071 742 465 399 334 168 85 BspEI T'CCGG_A Cuts at: 6660 6660 Size: 6997 BspHI T'CATG_A Cuts at: 3162 4886 6616 3162 Size: 1724 1730 3543 Fragments arranged by size: 3543 1730 1724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2237 2444 4192 4216 4352 844 Size: 1393 207 1748 24 136 3489 Fragments arranged by size: 3489 1748 1393 207 136 24 BsrBI CCG'CTC Cuts at: 1394 1823 2489 2640 4884 1394 Size: 429 666 151 2244 3507 Fragments arranged by size: 3507 2244 666 429 151 BsrDI GCAATG_nn' Cuts at: 65 1248 5492 5666 65 Size: 1183 4244 174 1396 Fragments arranged by size: 4244 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2451 5641 5828 1700 Size: 110 641 3190 187 2869 Fragments arranged by size: 3190 2869 641 187 110 BsrGI T'GTAC_A Cuts at: 96 3090 96 Size: 2994 4003 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6997 BssSI C'ACGA_G Cuts at: 3527 5057 6069 3527 Size: 1530 1012 4455 Fragments arranged by size: 4455 1530 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6997 BstBI TT'CG_AA Cuts at: 5801 5801 Size: 6997 BstEII G'GTnAC_C Cuts at: 1184 4030 1184 Size: 2846 4151 BstXI CCAn_nnnn'nTGG Cuts at: 2447 2447 Size: 6997 BstYI r'GATC_y Cuts at: 1972 2108 2234 2382 3216 3498 4327 4694 5079 Size: 136 126 148 834 282 829 367 385 Cuts at: 5079 5096 6548 6634 6646 6992 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 834 829 385 367 346 282 148 136 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2084 3485 513 Size: 552 939 80 1401 4025 Fragments arranged by size: 4025 1401 939 552 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6258 Fragments arranged by size: 6258 683 56 BtsI GCAGTG_nn' Cuts at: 2392 2572 3093 4513 5307 5327 2392 Size: 180 521 1420 794 20 4062 Fragments arranged by size: 4062 1420 794 521 180 20 ClaI AT'CG_AT Cuts at: 1554 3411 1554 Size: 1857 5140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2514 2549 529 Size: 35 1950 35 4977 Fragments arranged by size: 4977 1950 35 35 DraI TTT'AAA Cuts at: 4301 4301 Size: 6997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6004 809 Size: 507 4688 1802 Fragments arranged by size: 4688 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 2694 3107 Size: 54 1425 50 275 18 619 245 413 Cuts at: 3107 3769 4444 5333 6962 8 Size: 662 675 889 1629 43 Fragments arranged by size: 1629 1425 889 675 662 619 413 275 245 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4444 1537 Size: 275 2632 4090 Fragments arranged by size: 4090 2632 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3858 4927 1353 Size: 2505 1069 3423 Fragments arranged by size: 3423 2505 1069 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5584 5958 6104 387 Size: 1064 138 3995 374 146 1280 Fragments arranged by size: 3995 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2141 2198 2269 2279 2784 3000 Size: 393 453 56 57 71 10 505 216 Cuts at: 3000 3065 3147 3668 4074 4280 5060 5657 6444 Size: 65 82 521 406 206 780 597 787 Cuts at: 6444 6712 1239 Size: 268 1524 Fragments arranged by size: 1524 787 780 597 521 505 453 406 393 268 216 206 82 71 65 57 56 10 EcoNI CCTnn'n_nnAGG Cuts at: 3126 3126 Size: 6997 EcoO109I rG'GnC_Cy Cuts at: 2244 3076 3193 3643 2244 Size: 832 117 450 5598 Fragments arranged by size: 5598 832 450 117 EcoRI G'AATT_C Cuts at: 3708 4306 4684 3708 Size: 598 378 6021 Fragments arranged by size: 6021 598 378 EcoRV GAT'ATC Cuts at: 3886 3886 Size: 6997 FspI TGC'GCA Cuts at: 5503 5503 Size: 6997 HaeII r_GCGC'y Cuts at: 1678 6144 1678 Size: 4466 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2065 2097 2369 2401 5638 Size: 32 135 32 493 32 272 32 3237 Cuts at: 5638 5670 5712 5744 1373 Size: 32 42 32 2626 Fragments arranged by size: 3237 2626 493 272 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2887 4341 4587 669 Size: 988 281 949 1454 246 3079 Fragments arranged by size: 3079 1454 988 949 281 246 HindIII A'AGCT_T Cuts at: 748 2700 748 Size: 1952 5045 HpaI GTT'AAC Cuts at: 4587 4587 Size: 6997 KpnI G_GTAC'C Cuts at: 3468 4208 4322 3468 Size: 740 114 6143 Fragments arranged by size: 6143 740 114 MfeI C'AATT_G Cuts at: 4596 4596 Size: 6997 MluI A'CGCG_T Cuts at: 6873 6873 Size: 6997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2176 2526 3860 6111 6295 1305 Size: 871 350 1334 2251 184 2007 Fragments arranged by size: 2251 2007 1334 871 350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3109 3771 10 Size: 54 1425 343 1277 662 3236 Fragments arranged by size: 3236 1425 1277 662 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2397 3161 4955 5314 5473 518 Size: 648 1231 764 1794 359 159 2042 Fragments arranged by size: 2042 1794 1231 764 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2608 3969 4158 4413 5090 6238 6483 1606 Size: 1002 1361 189 255 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 1002 677 255 245 189 NcoI C'CATG_G Cuts at: 513 1065 2004 2084 3485 513 Size: 552 939 80 1401 4025 Fragments arranged by size: 4025 1401 939 552 80 NdeI CA'TA_TG Cuts at: 387 2944 387 Size: 2557 4440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6997 NotI GC'GGCC_GC Cuts at: 4444 4444 Size: 6997 NspI r_CATG'y Cuts at: 1132 1229 3359 4355 5900 1132 Size: 97 2130 996 1545 2229 Fragments arranged by size: 2229 2130 1545 996 97 PasI CC'CwG_GG Cuts at: 1348 2104 2762 1348 Size: 756 658 5583 Fragments arranged by size: 5583 756 658 PciI A'CATG_T Cuts at: 3355 5896 3355 Size: 2541 4456 PflMI CCAn_nnn'nTGG Cuts at: 1108 2085 2176 2930 2964 1108 Size: 977 91 754 34 5141 Fragments arranged by size: 5141 977 754 91 34 PfoI T'CCnGG_A Cuts at: 2246 2790 3219 2246 Size: 544 429 6024 Fragments arranged by size: 6024 544 429 PmeI GTTT'AAAC Cuts at: 4301 4301 Size: 6997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5066 5098 6579 6611 5066 Size: 32 1481 32 5452 Fragments arranged by size: 5452 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2244 3193 3643 2244 Size: 949 450 5598 Fragments arranged by size: 5598 949 450 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6997 PsiI TTA'TAA Cuts at: 4567 4567 Size: 6997 PspXI vC'TCGA_Gb Cuts at: 3816 3816 Size: 6997 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2327 2359 2327 Size: 32 6965 PstI C_TGCA'G Cuts at: 830 2234 2579 4349 830 Size: 1404 345 1770 3478 Fragments arranged by size: 3478 1770 1404 345 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6997 PvuII CAG'CTG Cuts at: 3969 4158 3969 Size: 189 6808 SalI G'TCGA_C Cuts at: 1936 4339 1936 Size: 2403 4594 SanDI GG'GwC_CC Cuts at: 3193 3193 Size: 6997 SapI GCTCTTCn'nnn_ Cuts at: 3858 3858 Size: 6997 SbfI CC_TGCA'GG Cuts at: 4349 4349 Size: 6997 ScaI AGT'ACT Cuts at: 3560 5245 6957 3560 Size: 1685 1712 3600 Fragments arranged by size: 3600 1712 1685 SexAI A'CCwGG_T Cuts at: 2820 2820 Size: 6997 SfcI C'TryA_G Cuts at: 826 1045 2230 2575 4345 5480 6161 6352 826 Size: 219 1185 345 1770 1135 681 191 1471 Fragments arranged by size: 1770 1471 1185 1135 681 345 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6997 SmaI CCC'GGG Cuts at: 1872 2508 4324 1872 Size: 636 1816 4545 Fragments arranged by size: 4545 1816 636 SmlI C'TyrA_G Cuts at: 820 1017 1943 2265 2685 3749 3816 5101 6002 Size: 197 926 322 420 1064 67 1285 901 Cuts at: 6002 6264 820 Size: 262 1553 Fragments arranged by size: 1553 1285 1064 926 901 420 322 262 197 67 SnaBI TAC'GTA Cuts at: 493 4788 493 Size: 4295 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6997 SphI G_CATG'C Cuts at: 1132 4355 1132 Size: 3223 3774 SspI AAT'ATT Cuts at: 5 52 4921 5 Size: 47 4869 2081 Fragments arranged by size: 4869 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6997 StyI C'CwwG_G Cuts at: 513 1065 2004 2084 3257 3485 4436 513 Size: 552 939 80 1173 228 951 3074 Fragments arranged by size: 3074 1173 951 939 552 228 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5024 5041 5194 5379 5824 325 Size: 1255 3444 17 153 185 445 1498 Fragments arranged by size: 3444 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3090 3558 4049 Size: 274 80 33 51 1443 1113 468 491 Cuts at: 4049 5243 6955 96 Size: 1194 1712 138 Fragments arranged by size: 1712 1443 1194 1113 491 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2171 2203 6957 6989 2171 Size: 32 4754 32 2179 Fragments arranged by size: 4754 2179 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2561 3005 4007 1316 Size: 1245 444 1002 4306 Fragments arranged by size: 4306 1245 1002 444 XbaI T'CTAG_A Cuts at: 4333 4333 Size: 6997 XhoI C'TCGA_G Cuts at: 1943 3816 1943 Size: 1873 5124 XmaI C'CCGG_G Cuts at: 1870 2506 4322 1870 Size: 636 1816 4545 Fragments arranged by size: 4545 1816 636 XmnI GAAnn'nnTTC Cuts at: 5126 5126 Size: 6997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3118 4805 276 Size: 53 83 186 936 1584 1687 2468 Fragments arranged by size: 2468 1687 1584 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI