(Linear) (Six-base) MAPSORT of: KIEE1431.seq Check: 9348 from: 1 to: 2292 KIEE1431.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 439 2292 Size: 439 1853 AatII G_ACGT'C Cuts at: 0 1115 2292 Size: 1115 1177 AccI GT'mk_AC Cuts at: 0 1934 2292 Size: 1934 358 Acc65I G'GTAC_C Cuts at: 0 1459 2199 2292 Size: 1459 740 93 Fragments arranged by size: 1459 740 93 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 779 995 1142 2275 2292 Size: 274 505 216 147 1133 17 Fragments arranged by size: 1133 505 274 216 147 17 AflIII A'CryG_T Cuts at: 0 179 217 610 1350 2292 Size: 179 38 393 740 942 Fragments arranged by size: 942 740 393 179 38 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2219 2292 Size: 2219 73 AlwNI CAG_nnn'CTG Cuts at: 0 171 1422 2292 Size: 171 1251 870 Fragments arranged by size: 1251 870 171 ApoI r'AATT_y Cuts at: 0 956 1703 1711 2292 Size: 956 747 8 581 Fragments arranged by size: 956 747 581 8 AseI AT'TA_AT Cuts at: 0 1682 2292 Size: 1682 610 AvaI C'yCGr_G Cuts at: 0 501 1811 2213 2292 Size: 501 1310 402 79 Fragments arranged by size: 1310 501 402 79 BamHI G'GATC_C Cuts at: 0 103 2292 Size: 103 2189 BanI G'GyrC_C Cuts at: 0 1459 2199 2292 Size: 1459 740 93 Fragments arranged by size: 1459 740 93 BanII G_rGCy'C Cuts at: 0 418 2292 Size: 418 1874 BbsI GAAGACnn'nnnn_ Cuts at: 0 262 557 698 1040 2292 Size: 262 295 141 342 1252 Fragments arranged by size: 1252 342 295 262 141 BbvCI CC'TCA_GC Cuts at: 0 740 2292 Size: 740 1552 BclI T'GATC_A Cuts at: 0 1736 2292 Size: 1736 556 BglI GCCn_nnn'nGGC Cuts at: 0 1078 2292 Size: 1078 1214 BglII A'GATC_T Cuts at: 0 229 377 1493 2292 Size: 229 148 1116 799 Fragments arranged by size: 1116 799 229 148 Bme1580I G_kGCm'C Cuts at: 0 752 2292 Size: 752 1540 BmrI ACTGGGnnnn_n' Cuts at: 0 1008 2292 Size: 1008 1284 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1844 1994 2292 Size: 1844 150 298 Fragments arranged by size: 1844 298 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 136 193 264 1060 1663 2069 2292 Size: 80 56 57 71 796 603 406 223 Fragments arranged by size: 796 603 406 223 80 71 57 56 Bpu10I CC'TnA_GC Cuts at: 0 740 2292 Size: 740 1552 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 665 1729 2292 Size: 245 420 1064 563 Fragments arranged by size: 1064 563 420 245 BsaI GGTCTCn'nnnn_ Cuts at: 0 356 712 2292 Size: 356 356 1580 Fragments arranged by size: 1580 356 356 BsaAI yAC'GTr Cuts at: 0 180 220 2292 Size: 180 40 2072 Fragments arranged by size: 2072 180 40 BsaHI Gr'CG_yC Cuts at: 0 1112 2292 Size: 1112 1180 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 60 90 166 196 2292 Size: 60 30 76 30 2096 Fragments arranged by size: 2096 76 60 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1918 1921 2066 2292 Size: 1918 3 145 226 Fragments arranged by size: 1918 226 145 3 BseYI C'CCAG_C Cuts at: 0 599 2153 2292 Size: 599 1554 139 Fragments arranged by size: 1554 599 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 312 1779 1856 2292 Size: 232 80 1467 77 436 Fragments arranged by size: 1467 436 232 80 77 BsiHKAI G_wGCw'C Cuts at: 0 250 2292 Size: 250 2042 Bsp1286I G_dGCh'C Cuts at: 0 250 418 752 2292 Size: 250 168 334 1540 Fragments arranged by size: 1540 334 250 168 BspHI T'CATG_A Cuts at: 0 1157 2292 Size: 1157 1135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 232 439 2187 2211 2292 Size: 232 207 1748 24 81 Fragments arranged by size: 1748 232 207 81 24 BsrBI CCG'CTC Cuts at: 0 484 635 2292 Size: 484 151 1657 Fragments arranged by size: 1657 484 151 BsrFI r'CCGG_y Cuts at: 0 446 2292 Size: 446 1846 BsrGI T'GTAC_A Cuts at: 0 1085 2292 Size: 1085 1207 BssSI C'ACGA_G Cuts at: 0 1522 2292 Size: 1522 770 BstEII G'GTnAC_C Cuts at: 0 2025 2292 Size: 2025 267 BstXI CCAn_nnnn'nTGG Cuts at: 0 442 2292 Size: 442 1850 BstYI r'GATC_y Cuts at: 0 103 229 377 1211 1493 2292 Size: 103 126 148 834 282 799 Fragments arranged by size: 834 799 282 148 126 103 BtgI C'CryG_G Cuts at: 0 79 1480 2292 Size: 79 1401 812 Fragments arranged by size: 1401 812 79 BtsI GCAGTG_nn' Cuts at: 0 387 567 1088 2292 Size: 387 180 521 1204 Fragments arranged by size: 1204 521 387 180 ClaI AT'CG_AT Cuts at: 0 1406 2292 Size: 1406 886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 509 544 2292 Size: 509 35 1748 Fragments arranged by size: 1748 509 35 EaeI y'GGCC_r Cuts at: 0 444 689 1102 1764 2292 Size: 444 245 413 662 528 Fragments arranged by size: 662 528 444 413 245 EarI CTCTTCn'nnn_ Cuts at: 0 1853 2292 Size: 1853 439 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 136 193 264 274 779 995 1060 Size: 80 56 57 71 10 505 216 65 Cuts at: 1060 1142 1663 2069 2275 2292 Size: 82 521 406 206 17 Fragments arranged by size: 521 505 406 216 206 82 80 71 65 57 56 17 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 1121 2292 Size: 1121 1171 EcoO109I rG'GnC_Cy Cuts at: 0 239 1071 1188 1638 2292 Size: 239 832 117 450 654 Fragments arranged by size: 832 654 450 239 117 EcoRI G'AATT_C Cuts at: 0 1703 2292 Size: 1703 589 EcoRV GAT'ATC Cuts at: 0 1881 2292 Size: 1881 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 60 92 364 396 2292 Size: 60 32 272 32 1896 Fragments arranged by size: 1896 272 60 32 32 HincII GTy'rAC Cuts at: 0 882 2292 Size: 882 1410 HindIII A'AGCT_T Cuts at: 0 695 2292 Size: 695 1597 KpnI G_GTAC'C Cuts at: 0 1463 2203 2292 Size: 1463 740 89 Fragments arranged by size: 1463 740 89 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 171 521 1855 2292 Size: 171 350 1334 437 Fragments arranged by size: 1334 437 350 171 MscI TGG'CCA Cuts at: 0 1104 1766 2292 Size: 1104 662 526 Fragments arranged by size: 1104 662 526 MslI CAynn'nnrTG Cuts at: 0 392 1156 2292 Size: 392 764 1136 Fragments arranged by size: 1136 764 392 MspA1I CmG'CkG Cuts at: 0 603 1964 2153 2292 Size: 603 1361 189 139 Fragments arranged by size: 1361 603 189 139 NcoI C'CATG_G Cuts at: 0 79 1480 2292 Size: 79 1401 812 Fragments arranged by size: 1401 812 79 NdeI CA'TA_TG Cuts at: 0 939 2292 Size: 939 1353 NspI r_CATG'y Cuts at: 0 1354 2292 Size: 1354 938 PasI CC'CwG_GG Cuts at: 0 99 757 2292 Size: 99 658 1535 Fragments arranged by size: 1535 658 99 PciI A'CATG_T Cuts at: 0 1350 2292 Size: 1350 942 PflMI CCAn_nnn'nTGG Cuts at: 0 80 171 925 959 2292 Size: 80 91 754 34 1333 Fragments arranged by size: 1333 754 91 80 34 PfoI T'CCnGG_A Cuts at: 0 241 785 1214 2292 Size: 241 544 429 1078 Fragments arranged by size: 1078 544 429 241 PpuMI rG'GwC_Cy Cuts at: 0 239 1188 1638 2292 Size: 239 949 450 654 Fragments arranged by size: 949 654 450 239 PspXI vC'TCGA_Gb Cuts at: 0 1811 2292 Size: 1811 481 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 322 354 2292 Size: 322 32 1938 Fragments arranged by size: 1938 322 32 PstI C_TGCA'G Cuts at: 0 229 574 2292 Size: 229 345 1718 Fragments arranged by size: 1718 345 229 PvuII CAG'CTG Cuts at: 0 1964 2153 2292 Size: 1964 189 139 Fragments arranged by size: 1964 189 139 SanDI GG'GwC_CC Cuts at: 0 1188 2292 Size: 1188 1104 SapI GCTCTTCn'nnn_ Cuts at: 0 1853 2292 Size: 1853 439 ScaI AGT'ACT Cuts at: 0 1555 2292 Size: 1555 737 SexAI A'CCwGG_T Cuts at: 0 815 2292 Size: 815 1477 SfcI C'TryA_G Cuts at: 0 225 570 2292 Size: 225 345 1722 Fragments arranged by size: 1722 345 225 SmaI CCC'GGG Cuts at: 0 503 2292 Size: 503 1789 SmlI C'TyrA_G Cuts at: 0 260 680 1744 1811 2292 Size: 260 420 1064 67 481 Fragments arranged by size: 1064 481 420 260 67 StyI C'CwwG_G Cuts at: 0 79 1252 1480 2292 Size: 79 1173 228 812 Fragments arranged by size: 1173 812 228 79 TatI w'GTAC_w Cuts at: 0 1085 1553 2044 2292 Size: 1085 468 491 248 Fragments arranged by size: 1085 491 468 248 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 166 198 2292 Size: 166 32 2094 Fragments arranged by size: 2094 166 32 Tth111I GACn'n_nGTC Cuts at: 0 556 1000 2002 2292 Size: 556 444 1002 290 Fragments arranged by size: 1002 556 444 290 XhoI C'TCGA_G Cuts at: 0 1811 2292 Size: 1811 481 XmaI C'CCGG_G Cuts at: 0 501 2292 Size: 501 1791 ZraI GAC'GTC Cuts at: 0 1113 2292 Size: 1113 1179 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AflIII AlfI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI BtsI ClaI CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI PspXI PsrI PstI PvuII SanDI SapI ScaI SexAI SfcI SmaI SmlI StyI TatI TstI Tth111I XhoI XmaI ZraI Enzymes that do not cut: AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BlpI BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI DraI DraIII DrdI EagI EciI EcoICRI FalI FseI FspI FspAI HaeII HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XcmI XmnI