(Circular) (Six-base) MAPSORT of: pFN21AB6420.seq Check: 9881 from: 1 to: 5722 pFN21AB6420 5722 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2062 3065 1937 Size: 125 1003 4594 Fragments arranged by size: 4594 1003 125 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 2579 3785 5169 5437 1632 Size: 620 327 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 620 327 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 4621 5598 1203 Size: 3418 977 1327 Fragments arranged by size: 3418 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2114 2146 2931 2963 2114 Size: 32 785 32 4873 Fragments arranged by size: 4873 785 32 32 AlwNI CAG_nnn'CTG Cuts at: 2071 3465 5037 2071 Size: 1394 1572 2756 Fragments arranged by size: 2756 1572 1394 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 2879 3031 3257 3409 5518 1493 Size: 1386 152 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 226 152 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2319 2958 3047 1870 Size: 73 99 277 639 89 4545 Fragments arranged by size: 4545 639 277 99 89 73 AvrII C'CTAG_G Cuts at: 2990 2990 Size: 5722 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2245 3043 4498 618 Size: 325 217 627 458 798 1455 1842 Fragments arranged by size: 1842 1455 798 627 458 325 217 BanII G_rGCy'C Cuts at: 1391 2266 2320 2959 3041 1391 Size: 875 54 639 82 4072 Fragments arranged by size: 4072 875 639 82 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4751 BbvCI CC'TCA_GC Cuts at: 2013 2013 Size: 5722 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2300 2334 3913 3947 1903 Size: 34 363 34 1579 34 3678 Fragments arranged by size: 3678 1579 363 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 1548 Size: 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4333 136 Size: 107 122 71 631 605 2661 1525 Fragments arranged by size: 2661 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 2895 3139 2895 Size: 244 5478 Bme1580I G_kGCm'C Cuts at: 1790 3783 4939 1790 Size: 1993 1156 2573 Fragments arranged by size: 2573 1993 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3453 4411 442 Size: 416 53 83 399 24 2036 958 1753 Fragments arranged by size: 2036 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2703 4382 1239 Size: 1464 1679 2579 Fragments arranged by size: 2579 1679 1464 Bpu10I CC'TnA_GC Cuts at: 2013 2013 Size: 5722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 2739 3847 4712 5010 2171 Size: 568 1108 865 298 2883 Fragments arranged by size: 2883 1108 865 568 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 2609 5716 2609 Size: 3107 2615 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2845 4155 4553 4827 4974 5385 2845 Size: 1310 398 274 147 411 3182 Fragments arranged by size: 3182 1310 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 2501 2531 2931 Size: 30 165 30 711 30 357 30 400 Cuts at: 2931 2961 1178 Size: 30 3939 Fragments arranged by size: 3939 711 400 357 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5722 BseYI C'CCAG_C Cuts at: 2288 4925 5421 2288 Size: 2637 496 2589 Fragments arranged by size: 2637 2589 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3783 3868 4939 3783 Size: 85 1071 4566 Fragments arranged by size: 4566 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2320 2959 3041 3783 3868 4939 Size: 399 476 54 639 82 742 85 1071 Cuts at: 4939 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 639 476 399 85 82 54 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 3611 5341 3611 Size: 1730 3992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3609 1394 Size: 429 1786 3507 Fragments arranged by size: 3507 1786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2353 2532 4217 4391 65 Size: 1183 1105 179 1685 174 1396 Fragments arranged by size: 1685 1396 1183 1105 179 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 4366 4553 1700 Size: 110 292 2264 187 2869 Fragments arranged by size: 2869 2264 292 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 2052 4526 2052 Size: 2474 3248 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2652 3052 3419 3804 3821 5273 5359 5371 Size: 680 400 367 385 17 1452 86 12 Cuts at: 5371 5717 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 680 400 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2033 2430 513 Size: 552 939 29 397 3805 Fragments arranged by size: 3805 939 552 397 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 2415 3238 4032 4052 2415 Size: 823 794 20 4085 Fragments arranged by size: 4085 823 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3169 4058 5687 Size: 54 1425 50 275 18 1339 889 1629 Cuts at: 5687 8 Size: 43 Fragments arranged by size: 1629 1425 1339 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2205 2616 2677 2922 3652 1353 Size: 852 411 61 245 730 3423 Fragments arranged by size: 3423 852 730 411 245 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2579 2703 3785 4382 5169 5437 Size: 393 620 327 124 1082 597 787 268 Cuts at: 5437 1239 Size: 1524 Fragments arranged by size: 1524 1082 787 620 597 393 327 268 124 EcoNI CCTnn'n_nnAGG Cuts at: 2153 2153 Size: 5722 EcoRI G'AATT_C Cuts at: 2879 3031 3409 2879 Size: 152 378 5192 Fragments arranged by size: 5192 378 152 EcoRV GAT'ATC Cuts at: 2611 2611 Size: 5722 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2688 2688 Size: 5722 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4363 4395 4437 4469 1373 Size: 32 135 32 2791 32 42 32 2626 Fragments arranged by size: 2791 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2284 3066 3312 669 Size: 988 281 346 782 246 3079 Fragments arranged by size: 3079 988 782 346 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 4836 5020 1305 Size: 1182 2349 184 2007 Fragments arranged by size: 2349 2007 1182 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3900 Fragments arranged by size: 3900 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3680 4039 4198 518 Size: 648 2514 359 159 2042 Fragments arranged by size: 2514 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2035 2314 3138 3815 4963 5208 1606 Size: 423 6 279 824 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 423 279 245 6 NcoI C'CATG_G Cuts at: 513 1065 2004 2430 513 Size: 552 939 426 3805 Fragments arranged by size: 3805 939 552 426 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 3080 4625 1132 Size: 97 1851 1545 2229 Fragments arranged by size: 2229 1851 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5722 PfoI T'CCnGG_A Cuts at: 2108 2118 2108 Size: 10 5712 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2114 2146 2931 2963 3791 3823 5304 5336 2114 Size: 32 785 32 828 32 1481 32 2500 Fragments arranged by size: 2500 1481 828 785 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 2333 3292 2333 Size: 959 4763 PstI C_TGCA'G Cuts at: 830 3074 830 Size: 2244 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2029 2029 Size: 5722 SacII CC_GC'GG Cuts at: 2036 2036 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 3070 4205 4886 5077 826 Size: 219 2025 1135 681 191 1471 Fragments arranged by size: 2025 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 2960 3049 1872 Size: 1088 89 4545 Fragments arranged by size: 4545 1088 89 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2319 2754 3826 4727 4989 Size: 197 926 207 169 435 1072 901 262 Cuts at: 4989 820 Size: 1553 Fragments arranged by size: 1553 1072 926 901 435 262 207 197 169 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 2579 3646 5 Size: 47 2527 1067 2081 Fragments arranged by size: 2527 2081 1067 47 StuI AGG'CCT Cuts at: 1517 2716 1517 Size: 1199 4523 StyI C'CwwG_G Cuts at: 513 1065 2004 2430 2862 2990 3161 513 Size: 552 939 426 432 128 171 3074 Fragments arranged by size: 3074 939 552 432 426 171 128 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2999 3749 3766 3919 4104 4549 325 Size: 1255 1419 750 17 153 185 445 1498 Fragments arranged by size: 1498 1419 1255 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3968 5680 96 Size: 274 80 33 51 1443 1991 1712 138 Fragments arranged by size: 1991 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5682 5714 5682 Size: 32 5690 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 2299 3058 2299 Size: 759 4963 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2428 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 2319 1943 Size: 376 5346 XmaI C'CCGG_G Cuts at: 1870 2958 3047 1870 Size: 1088 89 4545 Fragments arranged by size: 4545 1088 89 XmnI GAAnn'nnTTC Cuts at: 2129 2729 3851 2129 Size: 600 1122 4000 Fragments arranged by size: 4000 1122 600 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI