(Circular) (Six-base) MAPSORT of: pFN21AB3649.seq Check: 609 from: 1 to: 6481 pFN21AB3649 6481 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3824 1937 Size: 1887 4594 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2702 3395 3456 3693 4544 5928 6196 1632 Size: 1070 693 61 237 851 1384 268 1917 Fragments arranged by size: 1917 1384 1070 851 693 268 237 61 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 5380 6357 1203 Size: 4177 977 1327 Fragments arranged by size: 4177 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 2897 3095 4224 5796 2897 Size: 198 1129 1572 3582 Fragments arranged by size: 3582 1572 1129 198 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 3738 3790 4016 4168 6277 1493 Size: 2245 52 226 152 2109 1697 Fragments arranged by size: 2245 2109 1697 226 152 52 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 2376 2855 3806 1870 Size: 73 433 479 951 4545 Fragments arranged by size: 4545 951 479 433 73 BamHI G'GATC_C Cuts at: 3811 4178 3811 Size: 367 6114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2489 3802 5257 618 Size: 325 217 627 702 1313 1455 1842 Fragments arranged by size: 1842 1455 1313 702 627 325 217 BanII G_rGCy'C Cuts at: 1391 3800 1391 Size: 2409 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 2690 2850 3042 3714 928 Size: 971 240 551 160 192 672 3695 Fragments arranged by size: 3695 971 672 551 240 192 160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4672 4706 1903 Size: 34 2735 34 3678 Fragments arranged by size: 3678 2735 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4373 5583 4373 Size: 1210 5271 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2677 3232 6476 2677 Size: 555 3244 2682 Fragments arranged by size: 3244 2682 555 BlpI GC'TnA_GC Cuts at: 3399 3898 3399 Size: 499 5982 Bme1580I G_kGCm'C Cuts at: 1790 4542 5698 1790 Size: 2752 1156 2573 Fragments arranged by size: 2752 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2923 3171 4212 Size: 416 53 83 399 24 1506 248 1041 Cuts at: 4212 5170 442 Size: 958 1753 Fragments arranged by size: 1753 1506 1041 958 416 399 248 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2175 5141 1239 Size: 936 2966 2579 Fragments arranged by size: 2966 2579 936 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4606 5471 5769 4606 Size: 865 298 5318 Fragments arranged by size: 5318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5144 882 Size: 617 3645 2219 Fragments arranged by size: 3645 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2825 2995 4272 493 Size: 711 1621 170 1277 2702 Fragments arranged by size: 2702 1621 1277 711 170 BsaBI GATnn'nnATC Cuts at: 2676 6475 2676 Size: 3799 2682 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4670 5305 275 Size: 53 83 186 936 2755 382 635 1451 Fragments arranged by size: 2755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2847 3568 4914 5312 5586 5733 6144 2847 Size: 721 1346 398 274 147 411 3184 Fragments arranged by size: 3184 1346 721 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2140 2143 2170 2173 3269 Size: 30 165 30 737 3 27 3 1096 Cuts at: 3269 3299 1178 Size: 30 4360 Fragments arranged by size: 4360 1096 737 165 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2148 2796 3051 3638 3641 3716 3743 1186 Size: 962 648 255 587 3 75 27 3924 Fragments arranged by size: 3924 962 648 587 255 75 27 3 BseYI C'CCAG_C Cuts at: 5684 6180 5684 Size: 496 5985 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2588 1163 Size: 573 852 5056 Fragments arranged by size: 5056 852 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4542 4627 5698 4542 Size: 85 1071 5325 Fragments arranged by size: 5325 1071 85 BsmI GAATG_Cn' Cuts at: 3992 4085 3992 Size: 93 6388 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3800 4542 4627 5698 1391 Size: 399 2010 742 85 1071 2174 Fragments arranged by size: 2174 2010 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 2065 4370 6100 2065 Size: 2305 1730 2446 Fragments arranged by size: 2446 2305 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3836 844 Size: 2992 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4976 5150 65 Size: 1183 3728 174 1396 Fragments arranged by size: 3728 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5125 5312 1700 Size: 110 3315 187 2869 Fragments arranged by size: 3315 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6481 BstBI TT'CG_AA Cuts at: 5285 5285 Size: 6481 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstXI CCAn_nnnn'nTGG Cuts at: 2913 3538 2913 Size: 625 5856 BstYI r'GATC_y Cuts at: 1972 2677 2887 3232 3811 4178 4563 4580 6032 Size: 705 210 345 579 367 385 17 1452 Cuts at: 6032 6118 6130 6476 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 705 579 385 367 346 345 210 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3021 3021 Size: 6481 BtgI C'CryG_G Cuts at: 513 1065 2004 3446 513 Size: 552 939 1442 3548 Fragments arranged by size: 3548 1442 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5742 Fragments arranged by size: 5742 683 56 BtsI GCAGTG_nn' Cuts at: 2151 3997 4791 4811 2151 Size: 1846 794 20 3821 Fragments arranged by size: 3821 1846 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3785 3785 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3928 4817 6446 Size: 54 1425 50 275 18 2098 889 1629 Cuts at: 6446 8 Size: 43 Fragments arranged by size: 2098 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2943 3432 3509 3576 3585 3594 3633 Size: 765 825 489 77 67 9 9 39 Cuts at: 3633 3645 3769 4411 1353 Size: 12 124 642 3423 Fragments arranged by size: 3423 825 765 642 489 124 77 67 39 12 9 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2175 2702 3395 3456 3693 4544 5141 Size: 393 543 527 693 61 237 851 597 Cuts at: 5141 5928 6196 1239 Size: 787 268 1524 Fragments arranged by size: 1524 851 787 693 597 543 527 393 268 237 61 EcoO109I rG'GnC_Cy Cuts at: 2836 3370 2836 Size: 534 5947 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3662 3662 Size: 6481 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 3445 5628 1678 Size: 1767 2183 2531 Fragments arranged by size: 2531 2183 1767 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2865 2897 3090 3122 3157 Size: 32 135 32 1293 32 193 32 35 Cuts at: 3157 3189 3269 3301 5122 5154 5196 5228 1373 Size: 32 80 32 1821 32 42 32 2626 Fragments arranged by size: 2626 1821 1293 193 135 80 42 35 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2761 3825 4071 669 Size: 988 281 823 1064 246 3079 Fragments arranged by size: 3079 1064 988 823 281 246 HindIII A'AGCT_T Cuts at: 748 2342 748 Size: 1594 4887 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2222 2641 5595 5779 1305 Size: 917 419 2954 184 2007 Fragments arranged by size: 2954 2007 917 419 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4659 Fragments arranged by size: 4659 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 4439 4798 4957 518 Size: 648 913 2360 359 159 2042 Fragments arranged by size: 2360 2042 913 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3434 3897 4574 5722 5967 1606 Size: 1828 463 677 1148 245 2120 Fragments arranged by size: 2120 1828 1148 677 463 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 2660 3839 5384 1132 Size: 97 1431 1179 1545 2229 Fragments arranged by size: 2229 1545 1431 1179 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 2770 1108 Size: 1662 4819 PfoI T'CCnGG_A Cuts at: 3094 3094 Size: 6481 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PmlI CAC'GTG Cuts at: 2825 2825 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2836 2836 Size: 6481 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6481 PsiI TTA'TAA Cuts at: 3141 4051 3141 Size: 910 5571 PstI C_TGCA'G Cuts at: 830 3833 830 Size: 3003 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 3434 3434 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SapI GCTCTTCn'nnn_ Cuts at: 3432 3432 Size: 6481 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SfcI C'TryA_G Cuts at: 826 1045 3474 3829 4964 5645 5836 826 Size: 219 2429 355 1135 681 191 1471 Fragments arranged by size: 2429 1471 1135 681 355 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4585 5486 5748 820 Size: 197 926 2642 901 262 1553 Fragments arranged by size: 2642 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2995 4272 493 Size: 2502 1277 2702 Fragments arranged by size: 2702 2502 1277 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 4405 5 Size: 47 4353 2081 Fragments arranged by size: 4353 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6481 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 3920 513 Size: 552 939 168 1748 3074 Fragments arranged by size: 3074 1748 939 552 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4508 4525 4678 4863 5308 325 Size: 1255 2928 17 153 185 445 1498 Fragments arranged by size: 2928 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4727 6439 96 Size: 274 80 33 51 1443 2750 1712 138 Fragments arranged by size: 2750 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2143 2175 6441 6473 2000 Size: 32 111 32 4266 32 2008 Fragments arranged by size: 4266 2008 111 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2114 1316 Size: 798 5683 XbaI T'CTAG_A Cuts at: 3817 3817 Size: 6481 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 4610 4610 Size: 6481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI