Restriction map for
pFN21AB3649
(Linear) (Six-base) MAPSORT of: KIBB3649.seq Check: 772 from: 1 to: 1776
KIBB3649.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1390 1451 1688 1776
Size: 697 693 61 237 88
Fragments arranged by size:
697 693 237 88 61
AlwNI CAG_nnn'CTG
Cuts at: 0 892 1090 1776
Size: 892 198 686
Fragments arranged by size:
892 686 198
ApoI r'AATT_y
Cuts at: 0 1733 1776
Size: 1733 43
AvaI C'yCGr_G
Cuts at: 0 371 850 1776
Size: 371 479 926
Fragments arranged by size:
926 479 371
BanI G'GyrC_C
Cuts at: 0 484 1776
Size: 484 1292
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 685 845 1037 1709 1776
Size: 134 551 160 192 672 67
Fragments arranged by size:
672 551 192 160 134 67
BglII A'GATC_T
Cuts at: 0 672 1227 1776
Size: 672 555 549
Fragments arranged by size:
672 555 549
BlpI GC'TnA_GC
Cuts at: 0 1394 1776
Size: 1394 382
BmrI ACTGGGnnnn_n'
Cuts at: 0 918 1166 1776
Size: 918 248 610
Fragments arranged by size:
918 610 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1776
Size: 170 1606
BsaAI yAC'GTr
Cuts at: 0 820 990 1776
Size: 820 170 786
Fragments arranged by size:
820 786 170
BsaBI GATnn'nnATC
Cuts at: 0 671 1776
Size: 671 1105
BsaWI w'CCGG_w
Cuts at: 0 842 1563 1776
Size: 842 721 213
Fragments arranged by size:
842 721 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 135 138 165 168 1264 1294 1776
Size: 135 3 27 3 1096 30 482
Fragments arranged by size:
1096 482 135 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 143 791 1046 1633 1636 1711 1738 1776
Size: 143 648 255 587 3 75 27 38
Fragments arranged by size:
648 587 255 143 75 38 27 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1776
Size: 583 1193
BspHI T'CATG_A
Cuts at: 0 60 1776
Size: 60 1716
BstXI CCAn_nnnn'nTGG
Cuts at: 0 908 1533 1776
Size: 908 625 243
Fragments arranged by size:
908 625 243
BstYI r'GATC_y
Cuts at: 0 672 882 1227 1776
Size: 672 210 345 549
Fragments arranged by size:
672 549 345 210
Bsu36I CC'TnA_GG
Cuts at: 0 1016 1776
Size: 1016 760
BtgI C'CryG_G
Cuts at: 0 1441 1776
Size: 1441 335
BtsI GCAGTG_nn'
Cuts at: 0 146 1776
Size: 146 1630
EarI CTCTTCn'nnn_
Cuts at: 0 113 938 1427 1504 1571 1580 1589 1628
Size: 113 825 489 77 67 9 9 39
Cuts at: 1628 1640 1764 1776
Size: 12 124 12
Fragments arranged by size:
825 489 124 113 77 67 39 12
12 9 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 697 1390 1451 1688 1776
Size: 170 527 693 61 237 88
Fragments arranged by size:
693 527 237 170 88 61
EcoO109I rG'GnC_Cy
Cuts at: 0 831 1365 1776
Size: 831 534 411
Fragments arranged by size:
831 534 411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1657 1776
Size: 1657 119
HaeII r_GCGC'y
Cuts at: 0 1440 1776
Size: 1440 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 860 892 1085 1117 1152 1184 1264 1296
Size: 860 32 193 32 35 32 80 32
Cuts at: 1296 1776
Size: 480
Fragments arranged by size:
860 480 193 80 35 32 32 32
32
HincII GTy'rAC
Cuts at: 0 756 1776
Size: 756 1020
HindIII A'AGCT_T
Cuts at: 0 337 1776
Size: 337 1439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 636 1776
Size: 217 419 1140
Fragments arranged by size:
1140 419 217
MslI CAynn'nnrTG
Cuts at: 0 74 1776
Size: 74 1702
MspA1I CmG'CkG
Cuts at: 0 1429 1776
Size: 1429 347
NspI r_CATG'y
Cuts at: 0 655 1776
Size: 655 1121
PflMI CCAn_nnn'nTGG
Cuts at: 0 765 1776
Size: 765 1011
PfoI T'CCnGG_A
Cuts at: 0 1089 1776
Size: 1089 687
PmlI CAC'GTG
Cuts at: 0 820 1776
Size: 820 956
PpuMI rG'GwC_Cy
Cuts at: 0 831 1776
Size: 831 945
PsiI TTA'TAA
Cuts at: 0 1136 1776
Size: 1136 640
PvuII CAG'CTG
Cuts at: 0 1429 1776
Size: 1429 347
SapI GCTCTTCn'nnn_
Cuts at: 0 1427 1776
Size: 1427 349
SfcI C'TryA_G
Cuts at: 0 1469 1776
Size: 1469 307
SnaBI TAC'GTA
Cuts at: 0 990 1776
Size: 990 786
StyI C'CwwG_G
Cuts at: 0 167 1776
Size: 167 1609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 138 170 1776
Size: 27 111 32 1606
Fragments arranged by size:
1606 111 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 109 1776
Size: 109 1667
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanI BbsI BglII BlpI
BmrI BpmI BsaAI BsaBI BsaWI BsaXI BseRI BsgI
BspHI BstXI BstYI Bsu36I BtgI BtsI EarI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII MmeI MslI
MspA1I NspI PflMI PfoI PmlI PpuMI PsiI PvuII
SapI SfcI SnaBI StyI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaHI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
XbaI XcmI XhoI XmaI XmnI ZraI