(Circular) (Six-base) MAPSORT of: pFN21AE5514.seq Check: 9739 from: 1 to: 6070 pFN21AE5514 6070 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 1937 Size: 1476 4594 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 3258 4133 5517 5785 1632 Size: 1065 561 875 1384 268 1917 Fragments arranged by size: 1917 1384 1065 875 561 268 AfeI AGC'GCT Cuts at: 3304 3304 Size: 6070 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 2523 4969 5946 1203 Size: 1320 2446 977 1327 Fragments arranged by size: 2446 1327 1320 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2173 1184 Size: 989 5081 AlwNI CAG_nnn'CTG Cuts at: 3813 5385 3813 Size: 1572 4498 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 2904 3379 3605 3757 5866 1493 Size: 1411 475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1411 475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 3395 1870 Size: 73 1452 4545 Fragments arranged by size: 4545 1452 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2099 2132 2099 Size: 33 6037 BamHI G'GATC_C Cuts at: 2953 3400 3767 2953 Size: 447 367 5256 Fragments arranged by size: 5256 447 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2272 3346 3364 3391 4846 Size: 325 217 627 485 1074 18 27 1455 Cuts at: 4846 618 Size: 1842 Fragments arranged by size: 1842 1455 1074 627 485 325 217 27 18 BanII G_rGCy'C Cuts at: 1391 2175 3389 1391 Size: 784 1214 4072 Fragments arranged by size: 4072 1214 784 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5099 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 2601 3084 1548 Size: 1053 483 4534 Fragments arranged by size: 4534 1053 483 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4681 136 Size: 107 122 71 631 605 3009 1525 Fragments arranged by size: 3009 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 4131 5287 1790 Size: 2341 1156 2573 Fragments arranged by size: 2573 2341 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3801 4759 442 Size: 416 53 83 399 24 2384 958 1753 Fragments arranged by size: 2384 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2489 2489 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2008 4730 1239 Size: 769 2722 2579 Fragments arranged by size: 2722 2579 769 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4195 5060 5358 4195 Size: 865 298 4907 Fragments arranged by size: 4907 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4733 882 Size: 617 3234 2219 Fragments arranged by size: 3234 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3861 493 Size: 711 2657 2702 Fragments arranged by size: 2702 2657 711 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3877 4259 4894 275 Size: 53 83 186 936 2344 382 635 1451 Fragments arranged by size: 2344 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4503 4901 5175 5322 5733 4503 Size: 398 274 147 411 4840 Fragments arranged by size: 4840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3164 3194 1178 Size: 30 165 30 1761 30 4054 Fragments arranged by size: 4054 1761 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3172 1186 Size: 1986 4084 BseYI C'CCAG_C Cuts at: 5273 5769 5273 Size: 496 5574 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5497 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3520 4281 5309 1540 Size: 275 186 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 4131 4216 5287 2175 Size: 1956 85 1071 2958 Fragments arranged by size: 2958 1956 1071 85 BsmI GAATG_Cn' Cuts at: 3581 3674 3581 Size: 93 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 3389 4131 4216 5287 1391 Size: 399 385 1214 742 85 1071 2174 Fragments arranged by size: 2174 1214 1071 742 399 385 85 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 2031 3959 5689 2031 Size: 1928 1730 2412 Fragments arranged by size: 2412 1928 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3425 844 Size: 2581 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2634 4565 4739 65 Size: 1183 1386 1931 174 1396 Fragments arranged by size: 1931 1396 1386 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2122 96 Size: 2026 4044 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 4130 5142 4130 Size: 1012 5058 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstYI r'GATC_y Cuts at: 1972 2389 2421 2953 3400 3767 4152 4169 5621 Size: 417 32 532 447 367 385 17 1452 Cuts at: 5621 5707 5719 6065 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 532 447 417 385 367 346 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 2537 2537 Size: 6070 BtgI C'CryG_G Cuts at: 513 1065 2592 513 Size: 552 1527 3991 Fragments arranged by size: 3991 1527 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 3586 4380 4400 3586 Size: 794 20 5256 Fragments arranged by size: 5256 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 2095 3374 2095 Size: 1279 4791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3517 4406 6035 Size: 54 1425 50 275 18 1687 889 1629 Cuts at: 6035 8 Size: 43 Fragments arranged by size: 1687 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2487 3326 4000 1353 Size: 1134 839 674 3423 Fragments arranged by size: 3423 1134 839 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2173 2173 Size: 6070 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2697 3258 4133 4730 5517 5785 Size: 393 376 689 561 875 597 787 268 Cuts at: 5785 1239 Size: 1524 Fragments arranged by size: 1524 875 787 689 597 561 393 376 268 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 3306 5217 1678 Size: 1628 1911 2531 Fragments arranged by size: 2531 1911 1628 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3069 3101 4711 4743 4785 Size: 32 135 32 1497 32 1610 32 42 Cuts at: 4785 4817 1373 Size: 32 2626 Fragments arranged by size: 2626 1610 1497 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3414 3660 669 Size: 988 281 568 908 246 3079 Fragments arranged by size: 3079 988 908 568 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 2087 3669 2087 Size: 1582 4488 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2335 3028 5184 5368 1305 Size: 1030 693 2156 184 2007 Fragments arranged by size: 2156 2007 1030 693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4248 Fragments arranged by size: 4248 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2522 2597 4028 4387 4546 518 Size: 648 1356 75 1431 359 159 2042 Fragments arranged by size: 2042 1431 1356 648 359 159 75 MspA1I CmG'CkG Cuts at: 1606 2801 3486 4163 5311 5556 1606 Size: 1195 685 677 1148 245 2120 Fragments arranged by size: 2120 1195 1148 685 677 245 NcoI C'CATG_G Cuts at: 513 1065 2592 513 Size: 552 1527 3991 Fragments arranged by size: 3991 1527 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2527 3172 3428 4973 1132 Size: 97 1298 645 256 1545 2229 Fragments arranged by size: 2229 1545 1298 645 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 2523 4969 2523 Size: 2446 3624 PflMI CCAn_nnn'nTGG Cuts at: 1108 2417 1108 Size: 1309 4761 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PstI C_TGCA'G Cuts at: 830 3258 3422 830 Size: 2428 164 3478 Fragments arranged by size: 3478 2428 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 2801 2801 Size: 6070 SacI G_AGCT'C Cuts at: 2175 2175 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SapI GCTCTTCn'nnn_ Cuts at: 2487 2487 Size: 6070 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SfcI C'TryA_G Cuts at: 826 1045 3254 3418 4553 5234 5425 826 Size: 219 2209 164 1135 681 191 1471 Fragments arranged by size: 2209 1471 1135 681 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 3397 1872 Size: 1525 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4174 5075 5337 820 Size: 197 926 2231 901 262 1553 Fragments arranged by size: 2231 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 2858 2926 3994 5 Size: 47 2806 68 1068 2081 Fragments arranged by size: 2806 2081 1068 68 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2592 2817 3244 3509 513 Size: 552 1527 225 427 265 3074 Fragments arranged by size: 3074 1527 552 427 265 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2055 4097 4114 4267 4452 4897 325 Size: 1255 475 2042 17 153 185 445 1498 Fragments arranged by size: 2042 1498 1255 475 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2122 3171 4316 Size: 274 80 33 51 1443 145 1049 1145 Cuts at: 4316 6028 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1145 1049 274 145 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2682 2682 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 3395 1870 Size: 1525 4545 XmnI GAAnn'nnTTC Cuts at: 2906 4199 2906 Size: 1293 4777 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI