(Linear) (Six-base) MAPSORT of: KIEE5514.seq  Check: 291  from: 1  to: 1365



KIEE5514.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692    1253    1365 
   Size:        692     561     112

  Fragments arranged by size: 

                 692     561     112
 
AfeI AGC'GCT

Cuts at:      0    1299    1365 
   Size:       1299      66
 
AflIII A'CryG_T

Cuts at:      0     518    1365 
   Size:        518     847
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     168    1365 
   Size:        168    1197
 
ApoI r'AATT_y

Cuts at:      0     899    1365 
   Size:        899     466
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      94     127    1365 
   Size:         94      33    1238

  Fragments arranged by size: 

                1238      94      33
 
BamHI G'GATC_C

Cuts at:      0     948    1365 
   Size:        948     417
 
BanI G'GyrC_C

Cuts at:      0     267    1341    1359    1365 
   Size:        267    1074      18       6

  Fragments arranged by size: 

                1074     267      18       6
 
BanII G_rGCy'C

Cuts at:      0     170    1365 
   Size:        170    1195
 
BclI T'GATC_A

Cuts at:      0     596    1079    1365 
   Size:        596     483     286

  Fragments arranged by size: 

                 596     483     286
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     484    1365 
   Size:        484     881
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3    1365 
   Size:          3    1362
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1159    1189    1365 
   Size:       1159      30     176

  Fragments arranged by size: 

                1159     176      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1167    1365 
   Size:       1167     198
 
BsiHKAI G_wGCw'C

Cuts at:      0     170    1365 
   Size:        170    1195
 
Bsp1286I G_dGCh'C

Cuts at:      0     170    1365 
   Size:        170    1195
 
BspHI T'CATG_A

Cuts at:      0      26    1365 
   Size:         26    1339
 
BsrDI GCAATG_nn'

Cuts at:      0     629    1365 
   Size:        629     736
 
BsrGI T'GTAC_A

Cuts at:      0     117    1365 
   Size:        117    1248
 
BstYI r'GATC_y

Cuts at:      0     384     416     948    1365 
   Size:        384      32     532     417

  Fragments arranged by size: 

                 532     417     384      32
 
Bsu36I CC'TnA_GG

Cuts at:      0     532    1365 
   Size:        532     833
 
BtgI C'CryG_G

Cuts at:      0     587    1365 
   Size:        587     778
 
DraI TTT'AAA

Cuts at:      0      90    1365 
   Size:         90    1275
 
EarI CTCTTCn'nnn_

Cuts at:      0     482    1321    1365 
   Size:        482     839      44

  Fragments arranged by size: 

                 839     482      44
 
EcoICRI GAG'CTC

Cuts at:      0     168    1365 
   Size:        168    1197
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     692    1253    1365 
   Size:          3     689     561     112

  Fragments arranged by size: 

                 689     561     112       3
 
HaeII r_GCGC'y

Cuts at:      0    1301    1365 
   Size:       1301      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1064    1096    1365 
   Size:       1064      32     269

  Fragments arranged by size: 

                1064     269      32
 
HincII GTy'rAC

Cuts at:      0     501    1365 
   Size:        501     864
 
MfeI C'AATT_G

Cuts at:      0      82    1365 
   Size:         82    1283
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     330    1023    1365 
   Size:        330     693     342

  Fragments arranged by size: 

                 693     342     330
 
MslI CAynn'nnrTG

Cuts at:      0     517     592    1365 
   Size:        517      75     773

  Fragments arranged by size: 

                 773     517      75
 
MspA1I CmG'CkG

Cuts at:      0     796    1365 
   Size:        796     569
 
NcoI C'CATG_G

Cuts at:      0     587    1365 
   Size:        587     778
 
NspI r_CATG'y

Cuts at:      0     522    1167    1365 
   Size:        522     645     198

  Fragments arranged by size: 

                 645     522     198
 
PciI A'CATG_T

Cuts at:      0     518    1365 
   Size:        518     847
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     412    1365 
   Size:        412     953
 
PstI C_TGCA'G

Cuts at:      0    1253    1365 
   Size:       1253     112
 
PvuII CAG'CTG

Cuts at:      0     796    1365 
   Size:        796     569
 
SacI G_AGCT'C

Cuts at:      0     170    1365 
   Size:        170    1195
 
SapI GCTCTTCn'nnn_

Cuts at:      0     482    1365 
   Size:        482     883
 
SfcI C'TryA_G

Cuts at:      0    1249    1365 
   Size:       1249     116
 
SspI AAT'ATT

Cuts at:      0     853     921    1365 
   Size:        853      68     444

  Fragments arranged by size: 

                 853     444      68
 
StyI C'CwwG_G

Cuts at:      0     587     812    1239    1365 
   Size:        587     225     427     126

  Fragments arranged by size: 

                 587     427     225     126
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      50    1365 
   Size:         50    1315
 
TatI w'GTAC_w

Cuts at:      0     117    1166    1365 
   Size:        117    1049     199

  Fragments arranged by size: 

                1049     199     117
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     677    1365 
   Size:        677     688
 
XmnI GAAnn'nnTTC

Cuts at:      0     901    1365 
   Size:        901     464
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AlfI     ApoI     BaeI    BamHI     BanI
    BanII     BclI     BplI     BpmI    BsaXI    BseRI  BsiHKAI Bsp1286I
    BspHI    BsrDI    BsrGI    BstYI   Bsu36I     BtgI     DraI     EarI
  EcoICRI  Eco57MI    HaeII    Hin4I   HincII     MfeI     MmeI     MslI
   MspA1I     NcoI     NspI     PciI    PflMI     PstI    PvuII     SacI
     SapI     SfcI     SspI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI