################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## Acc65I AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI FauI FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MscI MspA1I MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 337 GACGTC GACGT^C AccI 259 GTCTAC GT^[AC][GT]AC AccII 453 CGCG CG^CG AccIII 296 TCCGGA T^CCGGA AciI 78 CCGC CCGC(-3/-1) BanII 151 GAGCTC G[GA]GC[CT]^C BsaI 445 GAGACC GGTCTC(1/5) BseAI 296 TCCGGA T^CCGGA BspEI 296 TCCGGA T^CCGGA BsrDI 241 GCAATG GCAATG(2/0) BsrFI 193 GCCGGT [GA]^CCGG[CT] BsrI 302 ACTGG ACTGG(1/-1) BstF5I 294 CATCC GGATG(2/0) BstUI 453 CGCG CG^CG CfoI 452 GCGC GCG^C Cfr10I 193 GCCGGT [GA]^CCGG[CT] DraI 133 TTTAAA TTT^AAA EarI 588 GAAGAG CTCTTC(1/4) EcoNI 227 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 502 GATATC GAT^ATC EcoT14I 395 CCAAGG C^C[AT][AT]GG EcoT22I 479 ATGCAT ATGCA^T FokI 294 CATCC GGATG(9/13) HgaI 91 GACGC GACGC(5/10) HhaI 452 GCGC GCG^C HinP1I 452 GCGC G^CGC HphI 269 TCACC GGTGA(8/7) Ksp632I 588 GAAGAG CTCTTC(1/4) MaeII 338 ACGT A^CGT MroI 296 TCCGGA T^CCGGA MslI 465 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 453 CGCG CG^CG NsiI 479 ATGCAT ATGCA^T PshAI 63 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 151 GAGCTC GAGCT^C SfaNI 248 GCATC GCATC(5/9) StyI 395 CCAAGG C^C[AT][AT]GG XcmI 461 CCAACATCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 91 GACGCC G[GA]^CG[CT]C 337 GACGTC Asp700I 202 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 279 GAAGTCCTTC AspHI 151 GAGCTC G[AT]GC[AT]^C 322 GAGCAC AvaI 48 CTCGGG C^[CT]CG[GA]G 355 CTCGGG AvaII 143 GGACC G^G[AT]CC 579 GGACC BbsI 6 GAAGAC GAAGAC(2/6) 60 GAAGAC BmyI 151 GAGCTC G[AGT]GC[ACT]^C 322 GAGCAC BpuAI 6 GAAGAC GAAGAC(2/6) 60 GAAGAC BsaHI 91 GACGCC G[GA]^CG[CT]C 337 GACGTC BsaWI 296 TCCGGA [AT]^CCGG[AT] 576 ACCGGA BseRI 319 GAGGAG GAGGAG(10/8) 591 GAGGAG BsiHKAI 151 GAGCTC G[AT]GC[AT]^C 322 GAGCAC BsiYI 227 CCTACTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 395 CCAAGGAACGG BslI 227 CCTACTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 395 CCAAGGAACGG BsmAI 384 GAGAC GTCTC(1/5) 445 GAGAC BsmFI 44 GGGAC GGGAC(10/14) 185 GGGAC BsoBI 48 CTCGGG C^[CT]CG[GA]G 355 CTCGGG Bsp1286I 151 GAGCTC G[AGT]GC[ACT]^C 322 GAGCAC BssKI 546 CCAGG ^CC[ACGT]GG 558 CCAGG BstNI 546 CCAGG CC^[AT]GG 558 CCAGG Cac8I 324 GCACGC GC[ACGT]^[ACGT]GC 414 GCTGGC DpnII 39 GATC ^GATC 216 GATC EcoRII 546 CCAGG ^CC[AT]GG 558 CCAGG Hin1I 91 GACGCC G[GA]^CG[CT]C 337 GACGTC MboI 39 GATC ^GATC 216 GATC MseI 134 TTAA T^TAA 170 TTAA MvaI 546 CCAGG CC^[AT]GG 558 CCAGG NdeII 39 GATC ^GATC 216 GATC NlaIII 252 CATG CATG^ 441 CATG PleI 46 GACTC GAGTC(4/5) 563 GAGTC PstI 175 CTGCAG CTGCA^G 332 CTGCAG Sau3AI 39 GATC ^GATC 216 GATC ScrFI 546 CCAGG CC^[ACGT]GG 558 CCAGG TfiI 306 GATTC G^A[AT]TC 431 GAATC Tru9I 134 TTAA T^TAA 170 TTAA XmnI 202 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 279 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AluI BbvI BsaJI Cfr13I Fnu4HI HaeIII HapII HinfI HpaII ItaI MboII MnlI MspI Sau96I SfcI TaqI TseI Tsp509I TthHB8I