################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AspI AvrII BbeI BcgI BciVI BclI BlnI BsaBI BsiEI BsiWI BsmI BspDI BspLU11I BssHII Bst1107I BstAPI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII ScaI SfiI SfoI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 586 GTCGAC GT^[AC][GT]AC AcyI 1768 GACGCC G[GA]^CG[CT]C AflII 1581 CTTAAG C^TTAAG AocI 1351 CCTGAGG CC^T[ACGT]AGG Aor51HI 26 AGCGCT AGC^GCT ApaI 736 GGGCCC GGGCC^C AseI 1362 ATTAAT AT^TAAT AsnI 1362 ATTAAT AT^TAAT Asp700I 1506 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1757 TGCGCA TGC^GCA BamHI 1526 GGATCC G^GATCC BbrPI 822 CACGTG CAC^GTG BfaI 1210 CTAG C^TAG BfrI 1581 CTTAAG C^TTAAG BglI 109 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1487 AGATCT A^GATCT BlpI 1336 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1336 GCTAAGC GC^T[ACGT]AGC BsaAI 822 CACGTG [CT]AC^GT[GA] BsaHI 1768 GACGCC G[GA]^CG[CT]C Bsp1407I 1273 TGTACA T^GTACA BspHI 830 TCATGA T^CATGA BsrFI 156 GCCGGT [GA]^CCGG[CT] BsrGI 1273 TGTACA T^GTACA BstBI 806 TTCGAA TT^CGAA BstEII 399 GGTGACC G^GT[ACGT]ACC BstPI 399 GGTGACC G^GT[ACGT]ACC Bsu36I 1351 CCTGAGG CC^T[ACGT]AGG CelII 1336 GCTAAGC GC^T[ACGT]AGC Cfr10I 156 GCCGGT [GA]^CCGG[CT] DraIII 702 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1723 GACTCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 26 AGCGCT AGC^GCT Eco81I 1351 CCTGAGG CC^T[ACGT]AGG EcoO65I 399 GGTGACC G^GT[ACGT]ACC EcoRI 774 GAATTC G^AATTC FspI 1757 TGCGCA TGC^GCA HaeII 26 AGCGCT [GA]GCGC^[CT] Hin1I 1768 GACGCC G[GA]^CG[CT]C HincII 586 GTCGAC GT[CT]^[GA]AC HindII 586 GTCGAC GT[CT]^[GA]AC MaeI 1210 CTAG C^TAG MslI 1734 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1658 CCATGG C^CATGG NdeI 972 CATATG CA^TATG NspV 806 TTCGAA TT^CGAA PmaCI 822 CACGTG CAC^GTG PmlI 822 CACGTG CAC^GTG PshAI 1136 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1362 ATTAAT AT^TAAT PvuII 567 CAGCTG CAG^CTG RcaI 830 TCATGA T^CATGA SalI 586 GTCGAC G^TCGAC SbfI 543 CCTGCAGG CCTGCA^GG SexAI 581 ACCTGGT A^CC[AT]GGT SfuI 806 TTCGAA TT^CGAA SmlI 1581 CTTAAG C^T[CT][GA]AG Sse8387I 543 CCTGCAGG CCTGCA^GG SspBI 1273 TGTACA T^GTACA XbaI 1209 TCTAGA T^CTAGA XcmI 789 CCAGGGTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1506 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 524 CGCG CG^CG 1043 CGCG AccIII 458 TCCGGA T^CCGGA 1648 TCCGGA AcsI 774 GAATTC [GA]^AATT[CT] 1588 GAATTT AflIII 821 ACACGT A^C[GA][CT]GT 1270 ACGTGT ApoI 774 GAATTC [GA]^AATT[CT] 1588 GAATTT BalI 1665 TGGCCA TGG^CCA 1793 TGGCCA BanI 82 GGTGCC G^G[CT][GA]CC 630 GGTGCC BbvCI 318 CCTCAGC CCTCAGC(-5/-2) 363 CCTCAGC BseAI 458 TCCGGA T^CCGGA 1648 TCCGGA BseRI 12 CTCCTC GAGGAG(10/8) 204 CTCCTC BsgI 537 CTGCAC GTGCAG(16/14) 910 CTGCAC BsmBI 712 GAGACG CGTCTC(1/5) 764 GAGACG BspEI 458 TCCGGA T^CCGGA 1648 TCCGGA BsrBI 744 CCGCTC CCGCTC(-3/-3) 298 GAGCGG BsrDI 1332 CATTGC GCAATG(2/0) 923 GCAATG BssSI 653 CACGAG CACGAG(-5/-1) 940 CTCGTG BstUI 524 CGCG CG^CG 1043 CGCG BstXI 488 CCAGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 989 CCAATGATGTGG DsaI 915 CCACGG C^C[GA][CT]GG 1658 CCATGG EcoT14I 813 CCTTGG C^C[AT][AT]GG 1658 CCATGG MaeII 823 ACGT A^CGT 1270 ACGT MluNI 1665 TGGCCA TGG^CCA 1793 TGGCCA MroI 458 TCCGGA T^CCGGA 1648 TCCGGA MscI 1665 TGGCCA TGG^CCA 1793 TGGCCA MseI 1363 TTAA T^TAA 1582 TTAA MvnI 524 CGCG CG^CG 1043 CGCG NspI 899 ACATGC [GA]CATG^[CT] 1423 ACATGC PflMI 411 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 789 CCAGGGTCTGG PleI 1723 GACTC GAGTC(4/5) 656 GAGTC PpuMI 905 GGGACCT [GA]G^G[AT]CC[CT] 1316 GGGACCT PstI 544 CTGCAG CTGCA^G 844 CTGCAG SacI 532 GAGCTC GAGCT^C 1260 GAGCTC SapI 1380 GCTCTTC GCTCTTC(1/4) 1779 GCTCTTC SfcI 544 CTGCAG C^T[GA][CT]AG 844 CTGCAG StyI 813 CCTTGG C^C[AT][AT]GG 1658 CCATGG Tru9I 1363 TTAA T^TAA 1582 TTAA Van91I 411 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 789 CCAGGGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaI BsaJI BsaWI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmaI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI