Restriction map for
pFN21AB7975
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AvaI AvrII BanII BbrPI
BbsI BbvCI BcgI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsgI BsiWI BsmI BsoBI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EarI Eco81I EcoNI
EcoO65I EcoRI EcoRV FseI HindIII
HpaI KpnI Ksp632I KspI MamI
MluI MslI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 881 TCCGGA T^CCGGA
Alw44I 1012 GTGCAC G^TGCAC
ApaLI 1012 GTGCAC G^TGCAC
AviII 630 TGCGCA TGC^GCA
BalI 905 TGGCCA TGG^CCA
BamHI 765 GGATCC G^GATCC
BciVI 466 GTATCC GTATCC(6/5)
BclI 842 TGATCA T^GATCA
BfaI 499 CTAG C^TAG
BlpI 1170 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1170 GCTGAGC GC^T[ACGT]AGC
BsaI 658 GAGACC GGTCTC(1/5)
BseAI 881 TCCGGA T^CCGGA
BsmBI 679 GAGACG CGTCTC(1/5)
BspEI 881 TCCGGA T^CCGGA
BspHI 866 TCATGA T^CATGA
BsrFI 454 GCCGGC [GA]^CCGG[CT]
BssHII 17 GCGCGC G^CGCGC
CelII 1170 GCTGAGC GC^T[ACGT]AGC
Cfr10I 454 GCCGGC [GA]^CCGG[CT]
EagI 150 CGGCCG C^GGCCG
EclXI 150 CGGCCG C^GGCCG
Eco52I 150 CGGCCG C^GGCCG
EcoT22I 871 ATGCAT ATGCA^T
FbaI 842 TGATCA T^GATCA
FspI 630 TGCGCA TGC^GCA
HincII 694 GTCAAC GT[CT]^[GA]AC
HindII 694 GTCAAC GT[CT]^[GA]AC
MaeI 499 CTAG C^TAG
MaeIII 556 GTCAC ^GT[ACGT]AC
MfeI 332 CAATTG C^AATTG
MluNI 905 TGGCCA TGG^CCA
MroI 881 TCCGGA T^CCGGA
MscI 905 TGGCCA TGG^CCA
MseI 569 TTAA T^TAA
MunI 332 CAATTG C^AATTG
NaeI 454 GCCGGC GCC^GGC
NcoI 1157 CCATGG C^CATGG
NgoMIV 454 GCCGGC G^CCGGC
NsiI 871 ATGCAT ATGCA^T
PleI 989 GACTC GAGTC(4/5)
PvuII 948 CAGCTG CAG^CTG
RcaI 866 TCATGA T^CATGA
SmlI 205 CTCAAG C^T[CT][GA]AG
TaqI 1019 TCGA T^CGA
TfiI 537 GATTC G^A[AT]TC
Tru9I 569 TTAA T^TAA
Tsp45I 556 GTCAC ^GT[GC]AC
TthHB8I 1019 TCGA T^CGA
XbaI 498 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 981 GGCGCC G[GA]^CG[CT]C
1228 GGCGCC
AfaI 798 GTAC GT^AC
1026 GTAC
AhdI 133 GACCGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
343 GACTTGGTGTC
AlwNI 771 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1165 CAGTGGCTG
Aor51HI 419 AGCGCT AGC^GCT
1034 AGCGCT
AspEI 133 GACCGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
343 GACTTGGTGTC
AspI 59 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
166 GACCGCGTC
BbeI 981 GGCGCC GGCGC^C
1228 GGCGCC
BsaHI 981 GGCGCC G[GA]^CG[CT]C
1228 GGCGCC
BseRI 223 GAGGAG GAGGAG(10/8)
1108 GAGGAG
BsiEI 150 CGGCCG CG[GA][CT]^CG
165 CGACCG
BstXI 1032 CCAGCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1241 CCAGTCCCCTGG
BstYI 765 GGATCC [GA]^GATC[CT]
878 AGATCC
DsaI 291 CCACGG C^C[GA][CT]GG
1157 CCATGG
Eam1105I 133 GACCGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
343 GACTTGGTGTC
Eco47III 419 AGCGCT AGC^GCT
1034 AGCGCT
Hin1I 981 GGCGCC G[GA]^CG[CT]C
1228 GGCGCC
HinfI 537 GATTC G^A[ACGT]TC
989 GACTC
KasI 981 GGCGCC G^GCGCC
1228 GGCGCC
MflI 765 GGATCC [GA]^GATC[CT]
878 AGATCC
NarI 981 GGCGCC GG^CGCC
1228 GGCGCC
NspI 121 GCATGC [GA]CATG^[CT]
296 GCATGC
PflFI 59 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
166 GACCGCGTC
PstI 144 CTGCAG CTGCA^G
685 CTGCAG
RsaI 798 GTAC GT^AC
1026 GTAC
SfcI 144 CTGCAG C^T[GA][CT]AG
685 CTGCAG
SfoI 981 GGCGCC GGC^GCC
1228 GGCGCC
SphI 121 GCATGC GCATG^C
296 GCATGC
Tth111I 59 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
166 GACCGCGTC
XhoII 765 GGATCC [GA]^GATC[CT]
878 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BsaWI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp509I TspRI