(Circular) (Six-base) MAPSORT of: pFN21AB7975.seq Check: 7082 from: 1 to: 5986 pFN21AB7975 5986 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3212 3212 Size: 5986 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 2573 3184 4049 5433 5701 1632 Size: 866 75 611 865 1384 268 1917 Fragments arranged by size: 1917 1384 866 865 611 268 75 AfeI AGC'GCT Cuts at: 2426 3041 2426 Size: 615 5371 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 2143 2353 4715 2143 Size: 210 2362 3414 Fragments arranged by size: 3414 2362 210 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 2781 3175 3729 5301 2781 Size: 394 554 1572 3466 Fragments arranged by size: 3466 1572 554 394 ApaLI G'TGCA_C Cuts at: 3017 4043 5199 3017 Size: 1026 1156 3804 Fragments arranged by size: 3804 1156 1026 ApoI r'AATT_y Cuts at: 1493 3295 3521 3673 5782 1493 Size: 1802 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 3311 1870 Size: 73 1368 4545 Fragments arranged by size: 4545 1368 73 BamHI G'GATC_C Cuts at: 2770 3316 3683 2770 Size: 546 367 5073 Fragments arranged by size: 5073 546 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 3140 3233 3241 3307 Size: 325 217 627 1199 154 93 8 66 Cuts at: 3307 4762 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 154 93 66 8 BanII G_rGCy'C Cuts at: 1391 3305 1391 Size: 1914 4072 BbeI G_GCGC'C Cuts at: 2990 3237 2990 Size: 247 5739 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2482 3878 5088 2482 Size: 1396 1210 3380 Fragments arranged by size: 3380 1396 1210 BclI T'GATC_A Cuts at: 1548 2847 1548 Size: 1299 4687 BfrBI ATG'CAT Cuts at: 2878 2878 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 3176 3403 3176 Size: 227 5759 Bme1580I G_kGCm'C Cuts at: 1790 2729 2963 2982 3021 3246 4047 5203 1790 Size: 939 234 19 39 225 801 1156 2573 Fragments arranged by size: 2573 1156 939 801 234 225 39 19 BmgBI CAC'GTC Cuts at: 1318 2977 1318 Size: 1659 4327 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2769 3085 3163 Size: 416 53 83 399 24 1352 316 78 Cuts at: 3163 3240 3717 4675 442 Size: 77 477 958 1753 Fragments arranged by size: 1753 1352 958 477 416 399 316 83 78 77 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1997 2816 2855 2966 3029 4646 1239 Size: 758 819 39 111 63 1617 2579 Fragments arranged by size: 2579 1617 819 758 111 63 39 Bpu10I CC'TnA_GC Cuts at: 2675 2675 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 4111 4976 5274 2195 Size: 1916 865 298 2907 Fragments arranged by size: 2907 1916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2657 4649 882 Size: 617 1158 1992 2219 Fragments arranged by size: 2219 1992 1158 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2987 3234 3793 4175 Size: 53 83 186 936 1454 247 559 382 Cuts at: 4175 4810 275 Size: 635 1451 Fragments arranged by size: 1454 1451 936 635 559 382 247 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 2367 2886 4419 4817 5091 5238 5649 2139 Size: 228 519 1533 398 274 147 411 2476 Fragments arranged by size: 2476 1533 519 411 398 274 228 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3143 3173 1178 Size: 30 165 30 1740 30 3991 Fragments arranged by size: 3991 1740 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 3128 1186 Size: 1057 885 4044 Fragments arranged by size: 4044 1057 885 BseYI C'CCAG_C Cuts at: 2955 5189 5685 2955 Size: 2234 496 3256 Fragments arranged by size: 3256 2234 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2158 2173 3436 4197 5225 1540 Size: 275 186 157 15 1263 761 1028 2301 Fragments arranged by size: 2301 1263 1028 761 275 186 157 15 BsiHKAI G_wGCw'C Cuts at: 3021 3120 3182 4047 4132 5203 3021 Size: 99 62 865 85 1071 3804 Fragments arranged by size: 3804 1071 865 99 85 62 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 BsmBI CGTCTCn'nnnn_ Cuts at: 2678 2678 Size: 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2729 2963 2982 3021 3120 3182 3246 Size: 399 939 234 19 39 99 62 64 Cuts at: 3246 3305 4047 4132 5203 1391 Size: 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 939 742 399 234 99 85 64 62 59 39 19 BspEI T'CCGG_A Cuts at: 2886 5649 2886 Size: 2763 3223 BspHI T'CATG_A Cuts at: 2871 3875 5605 2871 Size: 1004 1730 3252 Fragments arranged by size: 3252 1730 1004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2622 2686 3212 3341 844 Size: 1778 64 526 129 3489 Fragments arranged by size: 3489 1778 526 129 64 BsrBI CCG'CTC Cuts at: 1394 1823 2217 2233 3148 3212 3873 1394 Size: 429 394 16 915 64 661 3507 Fragments arranged by size: 3507 915 661 429 394 64 16 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2459 4630 4817 1700 Size: 110 649 2171 187 2869 Fragments arranged by size: 2869 2171 649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 2022 1925 Size: 97 5889 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 3044 3253 3044 Size: 209 5777 BstYI r'GATC_y Cuts at: 1972 2770 2883 3316 3683 4068 4085 5537 5623 Size: 798 113 433 367 385 17 1452 86 Cuts at: 5623 5635 5981 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 798 433 385 367 346 113 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2296 3162 513 Size: 552 939 292 866 3337 Fragments arranged by size: 3337 939 866 552 292 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3268 3502 4296 4316 3268 Size: 234 794 20 4938 Fragments arranged by size: 4938 794 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2200 2235 529 Size: 35 1636 35 4280 Fragments arranged by size: 4280 1636 35 35 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2155 2461 Size: 54 1425 50 275 18 261 64 306 Cuts at: 2461 2910 3433 4322 5951 8 Size: 449 523 889 1629 43 Fragments arranged by size: 1629 1425 889 523 449 306 275 261 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2155 3433 1537 Size: 275 343 1278 4090 Fragments arranged by size: 4090 1278 343 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3916 1353 Size: 2563 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2347 4573 4947 5093 387 Size: 1064 138 758 2226 374 146 1280 Fragments arranged by size: 2226 1280 1064 758 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1997 2498 2573 2816 2855 2966 3029 Size: 393 365 501 75 243 39 111 63 Cuts at: 3029 3184 4049 4646 5433 5701 1239 Size: 155 865 597 787 268 1524 Fragments arranged by size: 1524 865 787 597 501 393 365 268 243 155 111 75 63 39 EcoO109I rG'GnC_Cy Cuts at: 2145 2319 2601 2830 2145 Size: 174 282 229 5301 Fragments arranged by size: 5301 282 229 174 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FspI TGC'GCA Cuts at: 2637 4492 2637 Size: 1855 4131 HaeII r_GCGC'y Cuts at: 1678 2428 2682 2990 3043 3237 5133 1678 Size: 750 254 308 53 194 1896 2531 Fragments arranged by size: 2531 1896 750 308 254 194 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2339 2371 3143 Size: 32 135 32 557 32 178 32 772 Cuts at: 3143 3175 4627 4659 4701 4733 1373 Size: 32 1452 32 42 32 2626 Fragments arranged by size: 2626 1452 772 557 178 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2701 3330 3576 669 Size: 988 281 763 629 246 3079 Fragments arranged by size: 3079 988 763 629 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KasI G'GCGC_C Cuts at: 2986 3233 2986 Size: 247 5739 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 2337 3585 2337 Size: 1248 4738 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2912 10 Size: 54 1425 343 1080 3084 Fragments arranged by size: 3084 1425 1080 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3944 4303 4462 518 Size: 648 2778 359 159 2042 Fragments arranged by size: 2778 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2154 2955 3070 3402 4079 5227 5472 1606 Size: 548 801 115 332 677 1148 245 2120 Fragments arranged by size: 2120 1148 801 677 548 332 245 115 NaeI GCC'GGC Cuts at: 2461 2461 Size: 5986 NarI GG'CG_CC Cuts at: 2987 3234 2987 Size: 247 5739 NcoI C'CATG_G Cuts at: 513 1065 2004 3162 513 Size: 552 939 1158 3337 Fragments arranged by size: 3337 1158 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NgoMIV G'CCGG_C Cuts at: 2459 2459 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NsiI A_TGCA'T Cuts at: 2880 2880 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2130 2305 3344 4889 1132 Size: 97 901 175 1039 1545 2229 Fragments arranged by size: 2229 1545 1039 901 175 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5986 PfoI T'CCnGG_A Cuts at: 2793 3006 2793 Size: 213 5773 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2145 2319 2601 2145 Size: 174 282 5530 Fragments arranged by size: 5530 282 174 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 2153 2694 3338 830 Size: 1323 541 644 3478 Fragments arranged by size: 3478 1323 644 541 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2955 2955 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 2149 2690 3334 4469 5150 5341 826 Size: 219 1104 541 644 1135 681 191 1471 Fragments arranged by size: 1471 1135 1104 681 644 541 219 191 SfoI GGC'GCC Cuts at: 2988 3235 2988 Size: 247 5739 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 4090 4991 5253 820 Size: 197 926 267 1880 901 262 1553 Fragments arranged by size: 1880 1553 926 901 267 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 2130 2305 3344 1132 Size: 998 175 1039 3774 Fragments arranged by size: 3774 1039 998 175 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2004 2982 3162 3237 3425 513 Size: 552 939 978 180 75 188 3074 Fragments arranged by size: 3074 978 939 552 188 180 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4013 4030 4183 4368 4813 325 Size: 1255 2433 17 153 185 445 1498 Fragments arranged by size: 2433 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2802 3030 4232 Size: 274 80 33 51 1443 825 228 1202 Cuts at: 4232 5944 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1202 825 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 2067 2174 1316 Size: 751 107 5128 Fragments arranged by size: 5128 751 107 XbaI T'CTAG_A Cuts at: 2503 3322 2503 Size: 819 5167 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI