Restriction map for
pFN21AB7975
(Linear) (Six-base) MAPSORT of: KIBB7975.seq Check: 9653 from: 1 to: 1281
KIBB7975.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1207 1281
Size: 1207 74
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 568 1179 1281
Size: 493 75 611 102
Fragments arranged by size:
611 493 102 75
AfeI AGC'GCT
Cuts at: 0 421 1036 1281
Size: 421 615 245
Fragments arranged by size:
615 421 245
AhdI GACnn_n'nnGTC
Cuts at: 0 138 348 1281
Size: 138 210 933
Fragments arranged by size:
933 210 138
AlwNI CAG_nnn'CTG
Cuts at: 0 776 1170 1281
Size: 776 394 111
Fragments arranged by size:
776 394 111
ApaLI G'TGCA_C
Cuts at: 0 1012 1281
Size: 1012 269
BamHI G'GATC_C
Cuts at: 0 765 1281
Size: 765 516
BanI G'GyrC_C
Cuts at: 0 981 1135 1228 1236 1281
Size: 981 154 93 8 45
Fragments arranged by size:
981 154 93 45 8
BbeI G_GCGC'C
Cuts at: 0 985 1232 1281
Size: 985 247 49
Fragments arranged by size:
985 247 49
BciVI GTATCCnnnnn_n'
Cuts at: 0 477 1281
Size: 477 804
BclI T'GATC_A
Cuts at: 0 842 1281
Size: 842 439
BfrBI ATG'CAT
Cuts at: 0 873 1281
Size: 873 408
BlpI GC'TnA_GC
Cuts at: 0 1171 1281
Size: 1171 110
Bme1580I G_kGCm'C
Cuts at: 0 724 958 977 1016 1241 1281
Size: 724 234 19 39 225 40
Fragments arranged by size:
724 234 225 40 39 19
BmgBI CAC'GTC
Cuts at: 0 972 1281
Size: 972 309
BmrI ACTGGGnnnn_n'
Cuts at: 0 764 1080 1158 1235 1281
Size: 764 316 78 77 46
Fragments arranged by size:
764 316 78 77 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 850 961 1024 1281
Size: 811 39 111 63 257
Fragments arranged by size:
811 257 111 63 39
Bpu10I CC'TnA_GC
Cuts at: 0 670 1281
Size: 670 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1281
Size: 190 1091
BsaI GGTCTCn'nnnn_
Cuts at: 0 652 1281
Size: 652 629
BsaHI Gr'CG_yC
Cuts at: 0 982 1229 1281
Size: 982 247 52
Fragments arranged by size:
982 247 52
BsaWI w'CCGG_w
Cuts at: 0 134 362 881 1281
Size: 134 228 519 400
Fragments arranged by size:
519 400 228 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1138 1168 1281
Size: 1138 30 113
Fragments arranged by size:
1138 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 1123 1281
Size: 238 885 158
Fragments arranged by size:
885 238 158
BseYI C'CCAG_C
Cuts at: 0 950 1281
Size: 950 331
BsiEI CG_ry'CG
Cuts at: 0 153 168 1281
Size: 153 15 1113
Fragments arranged by size:
1113 153 15
BsiHKAI G_wGCw'C
Cuts at: 0 1016 1115 1177 1281
Size: 1016 99 62 104
Fragments arranged by size:
1016 104 99 62
BsmBI CGTCTCn'nnnn_
Cuts at: 0 673 1281
Size: 673 608
Bsp1286I G_dGCh'C
Cuts at: 0 724 958 977 1016 1115 1177 1241 1281
Size: 724 234 19 39 99 62 64 40
Fragments arranged by size:
724 234 99 64 62 40 39 19
BspEI T'CCGG_A
Cuts at: 0 881 1281
Size: 881 400
BspHI T'CATG_A
Cuts at: 0 866 1281
Size: 866 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 617 681 1207 1281
Size: 617 64 526 74
Fragments arranged by size:
617 526 74 64
BsrBI CCG'CTC
Cuts at: 0 212 228 1143 1207 1281
Size: 212 16 915 64 74
Fragments arranged by size:
915 212 74 64 16
BsrFI r'CCGG_y
Cuts at: 0 454 1281
Size: 454 827
BssHII G'CGCG_C
Cuts at: 0 17 1281
Size: 17 1264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1039 1248 1281
Size: 1039 209 33
Fragments arranged by size:
1039 209 33
BstYI r'GATC_y
Cuts at: 0 765 878 1281
Size: 765 113 403
Fragments arranged by size:
765 403 113
BtgI C'CryG_G
Cuts at: 0 291 1157 1281
Size: 291 866 124
Fragments arranged by size:
866 291 124
BtsI GCAGTG_nn'
Cuts at: 0 1263 1281
Size: 1263 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 195 230 1281
Size: 195 35 1051
Fragments arranged by size:
1051 195 35
EaeI y'GGCC_r
Cuts at: 0 86 150 456 905 1281
Size: 86 64 306 449 376
Fragments arranged by size:
449 376 306 86 64
EagI C'GGCC_G
Cuts at: 0 150 1281
Size: 150 1131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 342 1281
Size: 342 939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 568 811 850 961 1024 1179 1281
Size: 493 75 243 39 111 63 155 102
Fragments arranged by size:
493 243 155 111 102 75 63 39
EcoO109I rG'GnC_Cy
Cuts at: 0 140 314 596 825 1281
Size: 140 174 282 229 456
Fragments arranged by size:
456 282 229 174 140
FspI TGC'GCA
Cuts at: 0 632 1281
Size: 632 649
HaeII r_GCGC'y
Cuts at: 0 423 677 985 1038 1232 1281
Size: 423 254 308 53 194 49
Fragments arranged by size:
423 308 254 194 53 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 334 366 1138 1170 1281
Size: 124 32 178 32 772 32 111
Fragments arranged by size:
772 178 124 111 32 32 32
HincII GTy'rAC
Cuts at: 0 696 1281
Size: 696 585
KasI G'GCGC_C
Cuts at: 0 981 1228 1281
Size: 981 247 53
Fragments arranged by size:
981 247 53
MfeI C'AATT_G
Cuts at: 0 332 1281
Size: 332 949
MscI TGG'CCA
Cuts at: 0 907 1281
Size: 907 374
MspA1I CmG'CkG
Cuts at: 0 149 950 1065 1281
Size: 149 801 115 216
Fragments arranged by size:
801 216 149 115
NaeI GCC'GGC
Cuts at: 0 456 1281
Size: 456 825
NarI GG'CG_CC
Cuts at: 0 982 1229 1281
Size: 982 247 52
Fragments arranged by size:
982 247 52
NcoI C'CATG_G
Cuts at: 0 1157 1281
Size: 1157 124
NgoMIV G'CCGG_C
Cuts at: 0 454 1281
Size: 454 827
NsiI A_TGCA'T
Cuts at: 0 875 1281
Size: 875 406
NspI r_CATG'y
Cuts at: 0 125 300 1281
Size: 125 175 981
Fragments arranged by size:
981 175 125
PfoI T'CCnGG_A
Cuts at: 0 788 1001 1281
Size: 788 213 280
Fragments arranged by size:
788 280 213
PpuMI rG'GwC_Cy
Cuts at: 0 140 314 596 1281
Size: 140 174 282 685
Fragments arranged by size:
685 282 174 140
PstI C_TGCA'G
Cuts at: 0 148 689 1281
Size: 148 541 592
Fragments arranged by size:
592 541 148
PvuII CAG'CTG
Cuts at: 0 950 1281
Size: 950 331
SfcI C'TryA_G
Cuts at: 0 144 685 1281
Size: 144 541 596
Fragments arranged by size:
596 541 144
SfoI GGC'GCC
Cuts at: 0 983 1230 1281
Size: 983 247 51
Fragments arranged by size:
983 247 51
SmlI C'TyrA_G
Cuts at: 0 205 1281
Size: 205 1076
SphI G_CATG'C
Cuts at: 0 125 300 1281
Size: 125 175 981
Fragments arranged by size:
981 175 125
StyI C'CwwG_G
Cuts at: 0 977 1157 1232 1281
Size: 977 180 75 49
Fragments arranged by size:
977 180 75 49
TatI w'GTAC_w
Cuts at: 0 797 1025 1281
Size: 797 228 256
Fragments arranged by size:
797 256 228
Tth111I GACn'n_nGTC
Cuts at: 0 62 169 1281
Size: 62 107 1112
Fragments arranged by size:
1112 107 62
XbaI T'CTAG_A
Cuts at: 0 498 1281
Size: 498 783
Enzymes that do cut:
AarI AcuI AfeI AhdI AlwNI ApaLI BamHI BanI
BbeI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssHII BstXI BstYI BtgI BtsI CspCI
EaeI EagI EciI Eco57MI EcoO109I FspI HaeII Hin4I
HincII KasI MfeI MscI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PfoI PpuMI PstI PvuII SfcI
SfoI SmlI SphI StyI TatI Tth111I XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbsI BbvCI BcgI BglI BglII
BmtI BplI BsaAI BsaBI BsgI BsiWI BsmI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KpnI MluI
MmeI MslI NdeI NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
XcmI XhoI XmaI XmnI ZraI