################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII AccI AccII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseRI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MaeI MfeI MluI MluNI MscI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 254 GGTACC G^GTACC AccIII 289 TCCGGA T^CCGGA AfaI 255 GTAC GT^AC ApaI 114 GGGCCC GGGCC^C Asp700I 787 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 254 GGTACC G^GTACC AvaII 76 GGTCC G^G[AT]CC BanI 254 GGTACC G^G[CT][GA]CC BcnI 36 CCCGG CC^[GC]GG BsaBI 588 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 289 TCCGGA [AT]^CCGG[AT] BseAI 289 TCCGGA T^CCGGA BsmAI 826 GAGAC GTCTC(1/5) BsmBI 826 GAGACG CGTCTC(1/5) BsmFI 28 GGGAC GGGAC(10/14) BspEI 289 TCCGGA T^CCGGA BspHI 134 TCATGA T^CATGA BsrDI 189 CATTGC GCAATG(2/0) BsrI 108 ACTGG ACTGG(1/-1) BstF5I 98 GGATG GGATG(2/0) BstYI 224 GGATCT [GA]^GATC[CT] Cac8I 625 GCTGGC GC[ACGT]^[ACGT]GC DraI 438 TTTAAA TTT^AAA EcoRI 357 GAATTC G^AATTC FokI 98 GGATG GGATG(9/13) HindIII 126 AAGCTT A^AGCTT HphI 865 TCACC GGTGA(8/7) KpnI 254 GGTACC GGTAC^C MaeII 655 ACGT A^CGT MamI 588 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 224 GGATCT [GA]^GATC[CT] MroI 289 TCCGGA T^CCGGA MslI 237 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 36 CCCGG CC^[GC]GG PleI 450 GAGTC GAGTC(4/5) RcaI 134 TCATGA T^CATGA RsaI 255 GTAC GT^AC SacI 467 GAGCTC GAGCT^C SapI 180 GCTCTTC GCTCTTC(1/4) SspI 418 AATATT AAT^ATT Tsp45I 244 GTGAC ^GT[GC]AC XhoII 224 GGATCT [GA]^GATC[CT] XmnI 787 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 467 GAGCTC G[AT]GC[AT]^C 657 GTGCTC BbvI 304 GCAGC GCAGC(8/12) 376 GCTGC BpmI 321 CTCCAG CTGGAG(16/14) 660 CTCCAG BsaJI 194 CCCTGG C^C[ACGT][ACGT]GG 195 CCTGGG BsiHKAI 467 GAGCTC G[AT]GC[AT]^C 657 GTGCTC DdeI 146 CTAAG C^T[ACGT]AG 518 CTGAG DraII 54 AGGCCCT [GA]G^G[ACGT]CC[CT] 114 GGGCCCC EcoO109I 54 AGGCCCT [GA]G^G[ACGT]CC[CT] 114 GGGCCCC Fnu4HI 304 GCAGC GC^[ACGT]GC 376 GCTGC HaeIII 55 GGCC GG^CC 115 GGCC HapII 37 CCGG C^CGG 290 CCGG HpaII 37 CCGG C^CGG 290 CCGG ItaI 304 GCAGC GC^[ACGT]GC 376 GCTGC MaeIII 244 GTGAC ^GT[ACGT]AC 548 GTTAC MspI 37 CCGG C^CGG 290 CCGG NlaIII 135 CATG CATG^ 405 CATG SfaNI 588 GATGC GCATC(5/9) 629 GCATC TseI 304 GCAGC G^C[AT]GC 376 GCTGC TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^ 584 CAGTG XcmI 165 CCAGATTATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 323 CCAGGAAGTGTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AcsI AluI AlwI ApoI BanII BbsI BmyI BpuAI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DpnII EarI EcoRII HinfI Ksp632I MboI MboII MnlI MseI MvaI NdeII NlaIV Sau3AI Sau96I ScrFI TfiI Tru9I Tsp509I