################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I EcoT22I FauI FbaI FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KspI MamI MfeI MroI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 649 GGTACC G^GTACC AccII 556 CGCG CG^CG AciI 1413 CCGC CCGC(-3/-1) AflIII 555 ACGCGT A^C[GA][CT]GT AhdI 1636 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 505 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1422 GGGCCC GGGCC^C Asp718I 649 GGTACC G^GTACC AspEI 1636 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2 TGGCCA TGG^CCA BciVI 869 GTATCC GTATCC(6/5) BlpI 283 GCTGAGC GC^T[ACGT]AGC Bpu1102I 283 GCTGAGC GC^T[ACGT]AGC BsgI 330 GTGCAG GTGCAG(16/14) Bsp1407I 1033 TGTACA T^GTACA BspMI 85 ACCTGC ACCTGC(4/8) BsrGI 1033 TGTACA T^GTACA BstUI 556 CGCG CG^CG CelII 283 GCTGAGC GC^T[ACGT]AGC CfoI 666 GCGC GCG^C DraIII 324 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2 TGGCCA [CT]^GGCC[GA] Eam1105I 1636 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 485 GAATTC G^AATTC EcoRV 1306 GATATC GAT^ATC HaeII 665 GGCGCT [GA]GCGC^[CT] HhaI 666 GCGC GCG^C HinP1I 666 GCGC G^CGC KpnI 649 GGTACC GGTAC^C MaeII 747 ACGT A^CGT MluI 555 ACGCGT A^CGCGT MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 594 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 556 CGCG CG^CG PleI 1181 GACTC GAGTC(4/5) PpuMI 71 AGGACCT [GA]G^G[AT]CC[CT] SacI 1144 GAGCTC GAGCT^C SapI 668 GCTCTTC GCTCTTC(1/4) ScaI 637 AGTACT AGT^ACT SfiI 1105 GGCCTTATCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1354 CTCAAG C^T[CT][GA]AG SspBI 1033 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Asp700I 472 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 538 GAAGGCTTTC AspHI 286 GAGCAC G[AT]GC[AT]^C 1144 GAGCTC AvaII 72 GGACC G^G[AT]CC 1390 GGACC BamHI 1123 GGATCC G^GATCC 1359 GGATCC BanI 649 GGTACC G^G[CT][GA]CC 1557 GGCACC BpmI 76 CTGGAG CTGGAG(16/14) 584 CTGGAG BseRI 45 CTCCTC GAGGAG(10/8) 587 GAGGAG BsiHKAI 286 GAGCAC G[AT]GC[AT]^C 1144 GAGCTC BsmAI 388 GAGAC GTCTC(1/5) 590 GAGAC BspHI 242 TCATGA T^CATGA 795 TCATGA BsrDI 594 CATTGC GCAATG(2/0) 1290 CATTGC DraII 71 AGGACCT [GA]G^G[ACGT]CC[CT] 1422 GGGCCCT EarI 669 CTCTTC CTCTTC(1/4) 1577 CTCTTC EcoO109I 71 AGGACCT [GA]G^G[ACGT]CC[CT] 1422 GGGCCCT HgaI 345 GCGTC GACGC(5/10) 557 GCGTC HphI 564 GGTGA GGTGA(8/7) 1617 GGTGA Ksp632I 669 CTCTTC CTCTTC(1/4) 1577 CTCTTC PshAI 153 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 177 GACAAATGTC RcaI 242 TCATGA T^CATGA 795 TCATGA TaqI 612 TCGA T^CGA 1497 TCGA TthHB8I 612 TCGA T^CGA 1497 TCGA XmnI 472 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 538 GAAGGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BanII BbvI BfaI BglI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII