(Circular) (Six-base) MAPSORT of: pFN21AE6437.seq Check: 525 from: 1 to: 6379 pFN21AE6437 6379 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2099 2099 Size: 6379 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 2654 3700 2654 Size: 1046 5333 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2661 4442 5826 6094 1632 Size: 1029 1781 1384 268 1917 Fragments arranged by size: 1917 1781 1384 1029 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 2560 5278 6255 1203 Size: 1357 2718 977 1327 Fragments arranged by size: 2718 1357 1327 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 3646 5108 3646 Size: 1462 4917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6379 AlwNI CAG_nnn'CTG Cuts at: 2515 4122 5694 2515 Size: 1607 1572 3200 Fragments arranged by size: 3200 1607 1572 ApaI G_GGCC'C Cuts at: 3431 3431 Size: 6379 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 2261 2282 2490 2843 2915 3284 3688 3914 Size: 768 21 208 353 72 369 404 226 Cuts at: 3914 4066 6175 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 768 404 369 353 226 208 152 72 21 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 3704 1870 Size: 73 1761 4545 Fragments arranged by size: 4545 1761 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2646 2679 2646 Size: 33 6346 BamHI G'GATC_C Cuts at: 3128 3364 3709 4076 3128 Size: 236 345 367 5431 Fragments arranged by size: 5431 367 345 236 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2654 3562 3700 5155 618 Size: 325 217 627 867 908 138 1455 1842 Fragments arranged by size: 1842 1455 908 867 627 325 217 138 BanII G_rGCy'C Cuts at: 1391 2088 3153 3431 3698 1391 Size: 697 1065 278 267 4072 Fragments arranged by size: 4072 1065 697 278 267 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2885 4271 5481 2885 Size: 1386 1210 3783 Fragments arranged by size: 3783 1386 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3117 3426 3560 Size: 107 122 71 631 605 1445 309 134 Cuts at: 3560 4990 136 Size: 1430 1525 Fragments arranged by size: 1525 1445 1430 631 605 309 134 122 107 71 BglII A'GATC_T Cuts at: 6374 6374 Size: 6379 BlpI GC'TnA_GC Cuts at: 2289 3796 2289 Size: 1507 4872 Bme1580I G_kGCm'C Cuts at: 1790 3431 3565 4440 5596 1790 Size: 1641 134 875 1156 2573 Fragments arranged by size: 2573 1641 1156 875 134 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3463 4110 5068 Size: 416 53 83 399 24 2046 647 958 Cuts at: 5068 442 Size: 1753 Fragments arranged by size: 2046 1753 958 647 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2610 5039 1239 Size: 863 508 2429 2579 Fragments arranged by size: 2579 2429 863 508 Bpu10I CC'TnA_GC Cuts at: 2628 2628 Size: 6379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3344 4504 5369 5667 3344 Size: 1160 865 298 4056 Fragments arranged by size: 4056 1160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5042 882 Size: 617 3543 2219 Fragments arranged by size: 3543 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4170 493 Size: 711 2966 2702 Fragments arranged by size: 2966 2702 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4186 4568 5203 275 Size: 53 83 186 936 2653 382 635 1451 Fragments arranged by size: 2653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4812 5210 5484 5631 6042 4812 Size: 398 274 147 411 5149 Fragments arranged by size: 5149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 1178 Size: 30 165 30 619 30 5505 Fragments arranged by size: 5505 619 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 2607 3690 1186 Size: 855 566 1083 3875 Fragments arranged by size: 3875 1083 855 566 BseYI C'CCAG_C Cuts at: 3338 5582 6078 3338 Size: 2244 496 3639 Fragments arranged by size: 3639 2244 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 1163 Size: 573 620 5186 Fragments arranged by size: 5186 620 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2295 3153 4440 4525 5596 2295 Size: 858 1287 85 1071 3078 Fragments arranged by size: 3078 1287 1071 858 85 BsmI GAATG_Cn' Cuts at: 3890 3983 3890 Size: 93 6286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2295 3153 3431 3565 3698 4440 Size: 399 298 207 858 278 134 133 742 Cuts at: 4440 4525 5596 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 858 742 399 298 278 207 134 133 85 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 2247 2800 4268 5998 2247 Size: 553 1468 1730 2628 Fragments arranged by size: 2628 1730 1468 553 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 3734 844 Size: 1255 1635 3489 Fragments arranged by size: 3489 1635 1255 BsrBI CCG'CTC Cuts at: 1394 1823 4266 1394 Size: 429 2443 3507 Fragments arranged by size: 3507 2443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2598 3294 4874 5048 65 Size: 1183 1350 696 1580 174 1396 Fragments arranged by size: 1580 1396 1350 1183 696 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5023 5210 1700 Size: 110 3213 187 2869 Fragments arranged by size: 3213 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3038 96 Size: 2942 3437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6379 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2511 2535 3128 3364 3709 4076 4461 4478 Size: 539 24 593 236 345 367 385 17 Cuts at: 4478 5930 6016 6028 6374 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 593 539 385 367 346 345 236 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2028 3895 4689 4709 2028 Size: 1867 794 20 3698 Fragments arranged by size: 3698 1867 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2077 2112 529 Size: 35 1513 35 4796 Fragments arranged by size: 4796 1513 35 35 DraI TTT'AAA Cuts at: 2503 3265 3495 3549 3683 2503 Size: 762 230 54 134 5199 Fragments arranged by size: 5199 762 230 134 54 DraIII CAC_nnn'GTG Cuts at: 2334 2334 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3826 4715 Size: 54 1425 50 275 18 177 1819 889 Cuts at: 4715 6344 8 Size: 1629 43 Fragments arranged by size: 1819 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2680 3588 4309 1353 Size: 1327 908 721 3423 Fragments arranged by size: 3423 1327 908 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3151 3151 Size: 6379 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2610 2661 4442 5039 5826 6094 Size: 393 470 508 51 1781 597 787 268 Cuts at: 6094 1239 Size: 1524 Fragments arranged by size: 1781 1524 787 597 508 470 393 268 51 EcoO109I rG'GnC_Cy Cuts at: 2077 3428 2077 Size: 1351 5028 EcoRI G'AATT_C Cuts at: 2490 3688 4066 2490 Size: 1198 378 4803 Fragments arranged by size: 4803 1198 378 EcoRV GAT'ATC Cuts at: 3313 3313 Size: 6379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3041 3085 3041 Size: 44 6335 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 2674 5526 1678 Size: 996 2852 2531 Fragments arranged by size: 2852 2531 996 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2782 2814 3632 3664 5020 Size: 32 135 32 1210 32 818 32 1356 Cuts at: 5020 5052 5094 5126 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 1210 818 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3723 3969 669 Size: 988 281 1785 246 3079 Fragments arranged by size: 3079 1785 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KpnI G_GTAC'C Cuts at: 2658 3704 2658 Size: 1046 5333 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 2560 6255 2560 Size: 3695 2684 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5493 5677 1305 Size: 4188 184 2007 Fragments arranged by size: 4188 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4380 Fragments arranged by size: 4380 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 4337 4696 4855 518 Size: 648 1437 1734 359 159 2042 Fragments arranged by size: 2042 1734 1437 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3795 4472 5620 5865 1606 Size: 2189 677 1148 245 2120 Fragments arranged by size: 2189 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 3737 5282 1132 Size: 97 2508 1545 2229 Fragments arranged by size: 2508 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 5278 5278 Size: 6379 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6379 PfoI T'CCnGG_A Cuts at: 2485 2485 Size: 6379 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2611 2643 4448 4480 5961 5993 2611 Size: 32 1805 32 1481 32 2997 Fragments arranged by size: 2997 1805 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2077 2077 Size: 6379 PshAI GACnn'nnGTC Cuts at: 1613 2162 2186 1613 Size: 549 24 5806 Fragments arranged by size: 5806 549 24 PsiI TTA'TAA Cuts at: 2697 3949 2697 Size: 1252 5127 PspOMI G'GGCC_C Cuts at: 3427 3427 Size: 6379 PstI C_TGCA'G Cuts at: 830 3731 830 Size: 2901 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 SacI G_AGCT'C Cuts at: 3153 3153 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SapI GCTCTTCn'nnn_ Cuts at: 2680 2680 Size: 6379 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 2644 4627 6339 2644 Size: 1983 1712 2684 Fragments arranged by size: 2684 1983 1712 SfcI C'TryA_G Cuts at: 826 1045 2722 2985 3635 3727 4862 5543 5734 Size: 219 1677 263 650 92 1135 681 191 Cuts at: 5734 826 Size: 1471 Fragments arranged by size: 1677 1471 1135 681 650 263 219 191 92 SfiI GGCCn_nnn'nGGCC Cuts at: 3117 3117 Size: 6379 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3359 4483 5384 5646 820 Size: 197 926 1416 1124 901 262 1553 Fragments arranged by size: 1553 1416 1124 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6379 StyI C'CwwG_G Cuts at: 513 1065 2004 3818 513 Size: 552 939 1814 3074 Fragments arranged by size: 3074 1814 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4406 4423 4576 4761 5206 325 Size: 1255 2826 17 153 185 445 1498 Fragments arranged by size: 2826 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2529 2642 3038 Size: 274 80 33 51 1443 552 113 396 Cuts at: 3038 3401 4625 6337 96 Size: 363 1224 1712 138 Fragments arranged by size: 1712 1443 1224 552 396 363 274 138 113 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 3339 3371 6339 6371 2022 Size: 32 1285 32 2968 32 2030 Fragments arranged by size: 2968 2030 1285 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 2481 2547 4508 2481 Size: 66 1961 4352 Fragments arranged by size: 4352 1961 66 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI