(Circular) (Six-base) MAPSORT of: pFN21AE5616.seq Check: 5161 from: 1 to: 6211 pFN21AE5616 6211 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3451 3451 Size: 6211 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3172 3554 1937 Size: 1235 382 4594 Fragments arranged by size: 4594 1235 382 Acc65I G'GTAC_C Cuts at: 2298 3532 2298 Size: 1234 4977 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2473 3269 3304 4274 5658 5926 1632 Size: 841 796 35 970 1384 268 1917 Fragments arranged by size: 1917 1384 970 841 796 268 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 5110 6087 1203 Size: 3907 977 1327 Fragments arranged by size: 3907 1327 977 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 2700 1166 Size: 1534 4677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3158 1184 Size: 1974 4237 AlwNI CAG_nnn'CTG Cuts at: 3215 3337 3391 3954 5526 3215 Size: 122 54 563 1572 3900 Fragments arranged by size: 3900 1572 563 122 54 ApaI G_GGCC'C Cuts at: 2503 2503 Size: 6211 ApaLI G'TGCA_C Cuts at: 4268 5424 4268 Size: 1156 5055 ApoI r'AATT_y Cuts at: 1493 3520 3746 3898 6007 1493 Size: 2027 226 152 2109 1697 Fragments arranged by size: 2109 2027 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 3536 1870 Size: 73 1593 4545 Fragments arranged by size: 4545 1593 73 BamHI G'GATC_C Cuts at: 3470 3541 3908 3470 Size: 71 367 5773 Fragments arranged by size: 5773 367 71 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2298 3532 4987 618 Size: 325 217 627 511 1234 1455 1842 Fragments arranged by size: 1842 1455 1234 627 511 325 217 BanII G_rGCy'C Cuts at: 1391 2503 2973 3530 1391 Size: 1112 470 557 4072 Fragments arranged by size: 4072 1112 557 470 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2479 2571 928 Size: 971 580 92 4568 Fragments arranged by size: 4568 971 580 92 BbvCI CC'TCA_GC Cuts at: 2268 2268 Size: 6211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4402 4436 1903 Size: 34 2465 34 3678 Fragments arranged by size: 3678 2465 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3388 4103 5313 3388 Size: 715 1210 4286 Fragments arranged by size: 4286 1210 715 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BfrBI ATG'CAT Cuts at: 2214 2214 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4822 136 Size: 107 122 71 631 605 3150 1525 Fragments arranged by size: 3150 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 2028 3341 3628 2028 Size: 1313 287 4611 Fragments arranged by size: 4611 1313 287 Bme1580I G_kGCm'C Cuts at: 1790 2503 2947 3008 4272 5428 1790 Size: 713 444 61 1264 1156 2573 Fragments arranged by size: 2573 1264 1156 713 444 61 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3942 4900 442 Size: 416 53 83 399 24 2525 958 1753 Fragments arranged by size: 2525 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6211 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2267 2267 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2300 2398 2802 2820 2829 3223 3463 Size: 876 185 98 404 18 9 394 240 Cuts at: 3463 4871 1239 Size: 1408 2579 Fragments arranged by size: 2579 1408 876 404 394 240 185 98 18 9 Bpu10I CC'TnA_GC Cuts at: 2079 2268 3216 2079 Size: 189 948 5074 Fragments arranged by size: 5074 948 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2477 2621 4336 5201 5499 2477 Size: 144 1715 865 298 3189 Fragments arranged by size: 3189 1715 865 298 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2385 4874 882 Size: 617 886 2489 2219 Fragments arranged by size: 2489 2219 886 617 BsaAI yAC'GTr Cuts at: 493 1204 4002 493 Size: 711 2798 2702 Fragments arranged by size: 2798 2702 711 BsaBI GATnn'nnATC Cuts at: 6205 6205 Size: 6211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4018 4400 5035 275 Size: 53 83 186 936 2485 382 635 1451 Fragments arranged by size: 2485 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2816 3453 3490 4644 5042 5316 5463 5874 2816 Size: 637 37 1154 398 274 147 411 3153 Fragments arranged by size: 3153 1154 637 411 398 274 147 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3407 3437 1178 Size: 30 165 30 2004 30 3952 Fragments arranged by size: 3952 2004 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6211 BseYI C'CCAG_C Cuts at: 5414 5910 5414 Size: 496 5715 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3164 3228 3404 1163 Size: 573 1428 64 176 3970 Fragments arranged by size: 3970 1428 573 176 64 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3037 3449 4272 4357 5428 3037 Size: 412 823 85 1071 3820 Fragments arranged by size: 3820 1071 823 412 85 BsmI GAATG_Cn' Cuts at: 3722 3815 3722 Size: 93 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2503 2947 2973 3008 3037 3449 3530 Size: 399 713 444 26 35 29 412 81 Cuts at: 3530 4272 4357 5428 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 713 444 412 399 85 81 35 29 26 BspEI T'CCGG_A Cuts at: 2816 3453 5874 2816 Size: 637 2421 3153 Fragments arranged by size: 3153 2421 637 BspHI T'CATG_A Cuts at: 4100 5830 4100 Size: 1730 4481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3451 3566 844 Size: 2607 115 3489 Fragments arranged by size: 3489 2607 115 BsrBI CCG'CTC Cuts at: 1394 1823 4098 1394 Size: 429 2275 3507 Fragments arranged by size: 3507 2275 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4706 4880 65 Size: 1183 3458 174 1396 Fragments arranged by size: 3458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2164 4855 5042 1700 Size: 110 354 2691 187 2869 Fragments arranged by size: 2869 2691 354 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6211 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 2626 1184 Size: 1442 4769 BstYI r'GATC_y Cuts at: 1972 3293 3470 3541 3908 4293 4310 5762 5848 Size: 1321 177 71 367 385 17 1452 86 Cuts at: 5848 5860 6206 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1321 385 367 346 177 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3377 3377 Size: 6211 BtgI C'CryG_G Cuts at: 513 1065 2013 2872 513 Size: 552 948 859 3852 Fragments arranged by size: 3852 948 859 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5472 Fragments arranged by size: 5472 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3727 4521 4541 3142 Size: 585 794 20 4812 Fragments arranged by size: 4812 794 585 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6176 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DraIII CAC_nnn'GTG Cuts at: 2429 2845 2429 Size: 416 5795 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3365 3658 4547 Size: 54 1425 50 275 18 1535 293 889 Cuts at: 4547 6176 8 Size: 1629 43 Fragments arranged by size: 1629 1535 1425 889 293 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2961 3323 4141 1353 Size: 1608 362 818 3423 Fragments arranged by size: 3423 1608 818 362 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4798 5172 5318 387 Size: 1064 138 3209 374 146 1280 Fragments arranged by size: 3209 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2300 2398 2473 2802 2820 2829 Size: 393 483 185 98 75 329 18 9 Cuts at: 2829 3223 3269 3304 3463 4274 4871 5658 5926 Size: 394 46 35 159 811 597 787 268 Cuts at: 5926 1239 Size: 1524 Fragments arranged by size: 1524 811 787 597 483 394 393 329 268 185 159 98 75 46 35 18 9 EcoNI CCTnn'n_nnAGG Cuts at: 2336 2336 Size: 6211 EcoRI G'AATT_C Cuts at: 3520 3898 3520 Size: 378 5833 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 5358 1678 Size: 3680 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 2379 2411 2910 Size: 32 135 32 541 32 234 32 499 Cuts at: 2910 2942 3285 3317 3407 3439 3462 3494 4852 Size: 32 343 32 90 32 23 32 1358 Cuts at: 4852 4884 4926 4958 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1358 541 499 343 234 135 90 42 32 32 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 3173 3555 3801 669 Size: 988 281 1235 382 246 3079 Fragments arranged by size: 3079 1235 988 382 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KpnI G_GTAC'C Cuts at: 2302 3536 2302 Size: 1234 4977 MfeI C'AATT_G Cuts at: 3273 3810 3273 Size: 537 5674 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5325 5509 1305 Size: 4020 184 2007 Fragments arranged by size: 4020 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3367 10 Size: 54 1425 343 1535 2854 Fragments arranged by size: 2854 1535 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2450 2700 4169 4528 4687 518 Size: 648 1236 48 250 1469 359 159 2042 Fragments arranged by size: 2042 1469 1236 648 359 250 159 48 MspA1I CmG'CkG Cuts at: 1606 2015 2111 2135 2700 2987 3201 3627 4304 Size: 409 96 24 565 287 214 426 677 Cuts at: 4304 5452 5697 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 426 409 287 245 214 96 24 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5659 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6211 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NsiI A_TGCA'T Cuts at: 2216 2216 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 3569 5114 1132 Size: 97 2340 1545 2229 Fragments arranged by size: 2340 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6211 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 2873 3201 1108 Size: 1765 328 4118 Fragments arranged by size: 4118 1765 328 PfoI T'CCnGG_A Cuts at: 2542 3473 2542 Size: 931 5280 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PspOMI G'GGCC_C Cuts at: 2499 2499 Size: 6211 PstI C_TGCA'G Cuts at: 830 2617 3108 3563 830 Size: 1787 491 455 3478 Fragments arranged by size: 3478 1787 491 455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 2135 2700 2987 3201 2135 Size: 565 287 214 5145 Fragments arranged by size: 5145 565 287 214 SacII CC_GC'GG Cuts at: 2016 2016 Size: 6211 SalI G'TCGA_C Cuts at: 1936 3171 3553 1936 Size: 1235 382 4594 Fragments arranged by size: 4594 1235 382 SapI GCTCTTCn'nnn_ Cuts at: 2961 2961 Size: 6211 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SexAI A'CCwGG_T Cuts at: 2841 2841 Size: 6211 SfcI C'TryA_G Cuts at: 826 1045 2613 2712 2899 3104 3559 4694 5375 Size: 219 1568 99 187 205 455 1135 681 Cuts at: 5375 5566 826 Size: 191 1471 Fragments arranged by size: 1568 1471 1135 681 455 219 205 191 187 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3538 1872 Size: 1666 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2456 2600 4315 5216 5478 820 Size: 197 926 513 144 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 513 262 197 144 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 4135 5 Size: 47 4083 2081 Fragments arranged by size: 4083 2081 47 StuI AGG'CCT Cuts at: 1517 2144 1517 Size: 627 5584 StyI C'CwwG_G Cuts at: 513 1065 2938 3650 513 Size: 552 1873 712 3074 Fragments arranged by size: 3074 1873 712 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4238 4255 4408 4593 5038 325 Size: 1255 2658 17 153 185 445 1498 Fragments arranged by size: 2658 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3408 4457 6169 Size: 274 80 33 51 1443 1431 1049 1712 Cuts at: 6169 96 Size: 138 Fragments arranged by size: 1712 1443 1431 1049 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2676 2708 6171 6203 2676 Size: 32 3463 32 2684 Fragments arranged by size: 3463 2684 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2091 2426 2530 2654 2091 Size: 335 104 124 5648 Fragments arranged by size: 5648 335 124 104 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 3536 1870 Size: 1666 4545 XmnI GAAnn'nnTTC Cuts at: 4340 4340 Size: 6211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI