(Linear) (Six-base) MAPSORT of: KIEE5616.seq Check: 4133 from: 1 to: 1506 KIEE5616.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1446 1506 Size: 1446 60 AccI GT'mk_AC Cuts at: 0 1167 1506 Size: 1167 339 Acc65I G'GTAC_C Cuts at: 0 293 1506 Size: 293 1213 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 468 1264 1299 1506 Size: 468 796 35 207 Fragments arranged by size: 796 468 207 35 AleI CACnn'nnGTG Cuts at: 0 695 1506 Size: 695 811 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1153 1506 Size: 1153 353 AlwNI CAG_nnn'CTG Cuts at: 0 1210 1332 1386 1506 Size: 1210 122 54 120 Fragments arranged by size: 1210 122 120 54 ApaI G_GGCC'C Cuts at: 0 498 1506 Size: 498 1008 BamHI G'GATC_C Cuts at: 0 1465 1506 Size: 1465 41 BanI G'GyrC_C Cuts at: 0 293 1506 Size: 293 1213 BanII G_rGCy'C Cuts at: 0 498 968 1506 Size: 498 470 538 Fragments arranged by size: 538 498 470 BbsI GAAGACnn'nnnn_ Cuts at: 0 474 566 1506 Size: 474 92 940 Fragments arranged by size: 940 474 92 BbvCI CC'TCA_GC Cuts at: 0 263 1506 Size: 263 1243 BciVI GTATCCnnnnn_n' Cuts at: 0 1383 1506 Size: 1383 123 BfrBI ATG'CAT Cuts at: 0 209 1506 Size: 209 1297 BlpI GC'TnA_GC Cuts at: 0 23 1336 1506 Size: 23 1313 170 Fragments arranged by size: 1313 170 23 Bme1580I G_kGCm'C Cuts at: 0 498 942 1003 1506 Size: 498 444 61 503 Fragments arranged by size: 503 498 444 61 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 262 1506 Size: 262 1244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 295 393 797 815 824 1218 1458 Size: 110 185 98 404 18 9 394 240 Cuts at: 1458 1506 Size: 48 Fragments arranged by size: 404 394 240 185 110 98 48 18 9 Bpu10I CC'TnA_GC Cuts at: 0 74 263 1211 1506 Size: 74 189 948 295 Fragments arranged by size: 948 295 189 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 616 1506 Size: 472 144 890 Fragments arranged by size: 890 472 144 BsaI GGTCTCn'nnnn_ Cuts at: 0 380 1506 Size: 380 1126 BsaWI w'CCGG_w Cuts at: 0 811 1448 1485 1506 Size: 811 637 37 21 Fragments arranged by size: 811 637 37 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1402 1432 1506 Size: 1402 30 74 Fragments arranged by size: 1402 74 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1159 1223 1399 1506 Size: 1159 64 176 107 Fragments arranged by size: 1159 176 107 64 BsiHKAI G_wGCw'C Cuts at: 0 1032 1444 1506 Size: 1032 412 62 Fragments arranged by size: 1032 412 62 Bsp1286I G_dGCh'C Cuts at: 0 498 942 968 1003 1032 1444 1506 Size: 498 444 26 35 29 412 62 Fragments arranged by size: 498 444 412 62 35 29 26 BspEI T'CCGG_A Cuts at: 0 811 1448 1506 Size: 811 637 58 Fragments arranged by size: 811 637 58 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1446 1506 Size: 1446 60 BsrFI r'CCGG_y Cuts at: 0 159 1506 Size: 159 1347 BstEII G'GTnAC_C Cuts at: 0 621 1506 Size: 621 885 BstYI r'GATC_y Cuts at: 0 1288 1465 1506 Size: 1288 177 41 Fragments arranged by size: 1288 177 41 Bsu36I CC'TnA_GG Cuts at: 0 1372 1506 Size: 1372 134 BtgI C'CryG_G Cuts at: 0 8 867 1506 Size: 8 859 639 Fragments arranged by size: 859 639 8 BtsI GCAGTG_nn' Cuts at: 0 1137 1506 Size: 1137 369 DraIII CAC_nnn'GTG Cuts at: 0 424 840 1506 Size: 424 416 666 Fragments arranged by size: 666 424 416 EaeI y'GGCC_r Cuts at: 0 1360 1506 Size: 1360 146 EarI CTCTTCn'nnn_ Cuts at: 0 956 1318 1506 Size: 956 362 188 Fragments arranged by size: 956 362 188 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 295 393 468 797 815 824 1218 Size: 110 185 98 75 329 18 9 394 Cuts at: 1218 1264 1299 1458 1506 Size: 46 35 159 48 Fragments arranged by size: 394 329 185 159 110 98 75 48 46 35 18 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 331 1506 Size: 331 1175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 108 140 374 406 905 937 1280 1312 Size: 108 32 234 32 499 32 343 32 Cuts at: 1312 1402 1434 1457 1489 1506 Size: 90 32 23 32 17 Fragments arranged by size: 499 343 234 108 90 32 32 32 32 32 32 23 17 HincII GTy'rAC Cuts at: 0 1168 1506 Size: 1168 338 KpnI G_GTAC'C Cuts at: 0 297 1506 Size: 297 1209 MfeI C'AATT_G Cuts at: 0 1268 1506 Size: 1268 238 MscI TGG'CCA Cuts at: 0 1362 1506 Size: 1362 144 MslI CAynn'nnrTG Cuts at: 0 397 445 695 1506 Size: 397 48 250 811 Fragments arranged by size: 811 397 250 48 MspA1I CmG'CkG Cuts at: 0 10 106 130 695 982 1196 1506 Size: 10 96 24 565 287 214 310 Fragments arranged by size: 565 310 287 214 96 24 10 NsiI A_TGCA'T Cuts at: 0 211 1506 Size: 211 1295 PflMI CCAn_nnn'nTGG Cuts at: 0 868 1196 1506 Size: 868 328 310 Fragments arranged by size: 868 328 310 PfoI T'CCnGG_A Cuts at: 0 537 1468 1506 Size: 537 931 38 Fragments arranged by size: 931 537 38 PspOMI G'GGCC_C Cuts at: 0 494 1506 Size: 494 1012 PstI C_TGCA'G Cuts at: 0 612 1103 1506 Size: 612 491 403 Fragments arranged by size: 612 491 403 PvuII CAG'CTG Cuts at: 0 130 695 982 1196 1506 Size: 130 565 287 214 310 Fragments arranged by size: 565 310 287 214 130 SacII CC_GC'GG Cuts at: 0 11 1506 Size: 11 1495 SalI G'TCGA_C Cuts at: 0 1166 1506 Size: 1166 340 SapI GCTCTTCn'nnn_ Cuts at: 0 956 1506 Size: 956 550 SexAI A'CCwGG_T Cuts at: 0 836 1506 Size: 836 670 SfcI C'TryA_G Cuts at: 0 608 707 894 1099 1506 Size: 608 99 187 205 407 Fragments arranged by size: 608 407 205 187 99 SmlI C'TyrA_G Cuts at: 0 451 595 1506 Size: 451 144 911 Fragments arranged by size: 911 451 144 StuI AGG'CCT Cuts at: 0 139 1506 Size: 139 1367 StyI C'CwwG_G Cuts at: 0 933 1506 Size: 933 573 TatI w'GTAC_w Cuts at: 0 1403 1506 Size: 1403 103 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 671 703 1506 Size: 671 32 803 Fragments arranged by size: 803 671 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 86 421 525 649 1506 Size: 86 335 104 124 857 Fragments arranged by size: 857 335 124 104 86 Enzymes that do cut: AarI AccI Acc65I AcuI AleI AlfI AlwNI ApaI BamHI BanI BanII BbsI BbvCI BciVI BfrBI BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstEII BstYI Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI Hin4I HincII KpnI MfeI MscI MslI MspA1I NsiI PflMI PfoI PspOMI PstI PvuII SacII SalI SapI SexAI SfcI SmlI StuI StyI TatI TstI XcmI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BclI BglI BglII BmgBI BmrI BmtI BsaAI BsaBI BsaHI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI