(Linear) (Six-base) MAPSORT of: KIEE3651.seq Check: 8143 from: 1 to: 2694 KIEE3651.orf With 182 enzymes: SgfI * December 27, 2011 16:37 .. AclI AA'CG_TT Cuts at: 0 718 2694 Size: 718 1976 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 340 1465 1802 2542 2694 Size: 37 303 1125 337 740 152 Fragments arranged by size: 1125 740 337 303 152 37 AfeI AGC'GCT Cuts at: 0 1300 2694 Size: 1300 1394 AflII C'TTAA_G Cuts at: 0 208 2694 Size: 208 2486 AflIII A'CryG_T Cuts at: 0 849 1994 2694 Size: 849 1145 700 Fragments arranged by size: 1145 849 700 AleI CACnn'nnGTG Cuts at: 0 137 2694 Size: 137 2557 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2465 2497 2694 Size: 2465 32 197 Fragments arranged by size: 2465 197 32 AlwNI CAG_nnn'CTG Cuts at: 0 325 1815 2694 Size: 325 1490 879 Fragments arranged by size: 1490 879 325 ApoI r'AATT_y Cuts at: 0 220 2655 2694 Size: 220 2435 39 Fragments arranged by size: 2435 220 39 AvaI C'yCGr_G Cuts at: 0 1967 2694 Size: 1967 727 BanI G'GyrC_C Cuts at: 0 1403 2694 Size: 1403 1291 BanII G_rGCy'C Cuts at: 0 2483 2694 Size: 2483 211 BbeI G_GCGC'C Cuts at: 0 1407 2694 Size: 1407 1287 BbsI GAAGACnn'nnnn_ Cuts at: 0 992 2694 Size: 992 1702 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1873 1907 2694 Size: 1873 34 787 Fragments arranged by size: 1873 787 34 BclI T'GATC_A Cuts at: 0 1859 2694 Size: 1859 835 BfrBI ATG'CAT Cuts at: 0 424 2694 Size: 424 2270 BglI GCCn_nnn'nGGC Cuts at: 0 2302 2694 Size: 2302 392 BglII A'GATC_T Cuts at: 0 1607 2694 Size: 1607 1087 BlpI GC'TnA_GC Cuts at: 0 318 2694 Size: 318 2376 Bme1580I G_kGCm'C Cuts at: 0 541 2694 Size: 541 2153 BmrI ACTGGGnnnn_n' Cuts at: 0 1251 2694 Size: 1251 1443 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1761 2522 2694 Size: 1761 761 172 Fragments arranged by size: 1761 761 172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 542 1453 1543 2080 2389 2467 2694 Size: 542 911 90 537 309 78 227 Fragments arranged by size: 911 542 537 309 227 90 78 Bpu10I CC'TnA_GC Cuts at: 0 312 1241 2694 Size: 312 929 1453 Fragments arranged by size: 1453 929 312 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1456 2194 2330 2354 2412 2545 2694 Size: 1456 738 136 24 58 133 149 Fragments arranged by size: 1456 738 149 136 133 58 24 BsaI GGTCTCn'nnnn_ Cuts at: 0 830 2694 Size: 830 1864 BsaAI yAC'GTr Cuts at: 0 850 2694 Size: 850 1844 BsaHI Gr'CG_yC Cuts at: 0 1404 2694 Size: 1404 1290 BsaWI w'CCGG_w Cuts at: 0 1286 2694 Size: 1286 1408 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1351 1381 2465 2495 2694 Size: 1351 30 1084 30 199 Fragments arranged by size: 1351 1084 199 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1164 2557 2694 Size: 1164 1393 137 Fragments arranged by size: 1393 1164 137 BseYI C'CCAG_C Cuts at: 0 1616 2694 Size: 1616 1078 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1975 2074 2694 Size: 1975 99 620 Fragments arranged by size: 1975 620 99 BsiHKAI G_wGCw'C Cuts at: 0 1069 1466 2249 2483 2520 2694 Size: 1069 397 783 234 37 174 Fragments arranged by size: 1069 783 397 234 174 37 BsmI GAATG_Cn' Cuts at: 0 678 2386 2694 Size: 678 1708 308 Fragments arranged by size: 1708 678 308 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1486 2694 Size: 1486 1208 Bsp1286I G_dGCh'C Cuts at: 0 541 1069 1466 2249 2483 2520 2694 Size: 541 528 397 783 234 37 174 Fragments arranged by size: 783 541 528 397 234 174 37 BspHI T'CATG_A Cuts at: 0 1136 2694 Size: 1136 1558 BsrBI CCG'CTC Cuts at: 0 1740 2694 Size: 1740 954 BsrDI GCAATG_nn' Cuts at: 0 683 695 2694 Size: 683 12 1999 Fragments arranged by size: 1999 683 12 BsrFI r'CCGG_y Cuts at: 0 839 2694 Size: 839 1855 BssSI C'ACGA_G Cuts at: 0 1870 2694 Size: 1870 824 BstAPI GCAn_nnn'nTGC Cuts at: 0 682 1941 2694 Size: 682 1259 753 Fragments arranged by size: 1259 753 682 BstXI CCAn_nnnn'nTGG Cuts at: 0 144 1521 2694 Size: 144 1377 1173 Fragments arranged by size: 1377 1173 144 BstYI r'GATC_y Cuts at: 0 499 1340 1607 1742 2016 2694 Size: 499 841 267 135 274 678 Fragments arranged by size: 841 678 499 274 267 135 Bsu36I CC'TnA_GG Cuts at: 0 59 785 2678 2694 Size: 59 726 1893 16 Fragments arranged by size: 1893 726 59 16 BtgI C'CryG_G Cuts at: 0 1224 1246 2141 2694 Size: 1224 22 895 553 Fragments arranged by size: 1224 895 553 22 BtsI GCAGTG_nn' Cuts at: 0 284 1092 2694 Size: 284 808 1602 Fragments arranged by size: 1602 808 284 DraIII CAC_nnn'GTG Cuts at: 0 850 1875 2694 Size: 850 1025 819 Fragments arranged by size: 1025 850 819 EaeI y'GGCC_r Cuts at: 0 841 1532 2694 Size: 841 691 1162 Fragments arranged by size: 1162 841 691 EarI CTCTTCn'nnn_ Cuts at: 0 1778 2534 2694 Size: 1778 756 160 Fragments arranged by size: 1778 756 160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 658 2694 Size: 658 2036 EcoICRI GAG'CTC Cuts at: 0 2481 2694 Size: 2481 213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 340 542 1453 1465 1543 1802 2080 Size: 37 303 202 911 12 78 259 278 Cuts at: 2080 2389 2467 2542 2694 Size: 309 78 75 152 Fragments arranged by size: 911 309 303 278 259 202 152 78 78 75 37 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 2560 2694 Size: 2560 134 EcoO109I rG'GnC_Cy Cuts at: 0 21 1275 1916 2694 Size: 21 1254 641 778 Fragments arranged by size: 1254 778 641 21 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 223 1813 2694 Size: 223 1590 881 Fragments arranged by size: 1590 881 223 FspI TGC'GCA Cuts at: 0 2512 2694 Size: 2512 182 HaeII r_GCGC'y Cuts at: 0 1302 1407 2694 Size: 1302 105 1287 Fragments arranged by size: 1302 1287 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 21 53 484 516 598 630 2328 2360 Size: 21 32 431 32 82 32 1698 32 Cuts at: 2360 2694 Size: 334 Fragments arranged by size: 1698 431 334 82 32 32 32 32 21 KasI G'GCGC_C Cuts at: 0 1403 2694 Size: 1403 1291 MfeI C'AATT_G Cuts at: 0 232 2694 Size: 232 2462 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2204 2694 Size: 2204 490 MscI TGG'CCA Cuts at: 0 1534 2694 Size: 1534 1160 MslI CAynn'nnrTG Cuts at: 0 137 419 1060 1349 2146 2474 2694 Size: 137 282 641 289 797 328 220 Fragments arranged by size: 797 641 328 289 282 220 137 MspA1I CmG'CkG Cuts at: 0 1248 1422 1989 2058 2694 Size: 1248 174 567 69 636 Fragments arranged by size: 1248 636 567 174 69 NarI GG'CG_CC Cuts at: 0 1404 2694 Size: 1404 1290 NcoI C'CATG_G Cuts at: 0 2141 2694 Size: 2141 553 NsiI A_TGCA'T Cuts at: 0 426 2694 Size: 426 2268 NspI r_CATG'y Cuts at: 0 1059 1101 1501 1939 2104 2694 Size: 1059 42 400 438 165 590 Fragments arranged by size: 1059 590 438 400 165 42 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1579 1611 2465 2497 2694 Size: 1579 32 854 32 197 Fragments arranged by size: 1579 854 197 32 32 PpuMI rG'GwC_Cy Cuts at: 0 21 1916 2694 Size: 21 1895 778 Fragments arranged by size: 1895 778 21 PsiI TTA'TAA Cuts at: 0 1391 2694 Size: 1391 1303 PstI C_TGCA'G Cuts at: 0 1581 2694 Size: 1581 1113 PvuII CAG'CTG Cuts at: 0 1422 1989 2058 2694 Size: 1422 567 69 636 Fragments arranged by size: 1422 636 567 69 SacI G_AGCT'C Cuts at: 0 2483 2694 Size: 2483 211 SacII CC_GC'GG Cuts at: 0 1249 2694 Size: 1249 1445 SfcI C'TryA_G Cuts at: 0 168 907 1477 1577 2398 2688 2694 Size: 168 739 570 100 821 290 6 Fragments arranged by size: 821 739 570 290 168 100 6 SfoI GGC'GCC Cuts at: 0 1405 2694 Size: 1405 1289 SmlI C'TyrA_G Cuts at: 0 208 1471 2209 2345 2369 2391 2524 2694 Size: 208 1263 738 136 24 22 133 170 Fragments arranged by size: 1263 738 208 170 136 133 24 22 SphI G_CATG'C Cuts at: 0 1101 1501 1939 2694 Size: 1101 400 438 755 Fragments arranged by size: 1101 755 438 400 SspI AAT'ATT Cuts at: 0 307 456 2694 Size: 307 149 2238 Fragments arranged by size: 2238 307 149 StuI AGG'CCT Cuts at: 0 1239 2389 2694 Size: 1239 1150 305 Fragments arranged by size: 1239 1150 305 StyI C'CwwG_G Cuts at: 0 2141 2694 Size: 2141 553 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1734 2694 Size: 1734 960 TatI w'GTAC_w Cuts at: 0 257 1817 1979 2063 2184 2694 Size: 257 1560 162 84 121 510 Fragments arranged by size: 1560 510 257 162 121 84 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 836 868 1325 1357 1945 1977 2694 Size: 836 32 457 32 588 32 717 Fragments arranged by size: 836 717 588 457 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 144 531 1542 2694 Size: 144 387 1011 1152 Fragments arranged by size: 1152 1011 387 144 XmnI GAAnn'nnTTC Cuts at: 0 1594 2694 Size: 1594 1100 Enzymes that do cut: AclI AcuI AfeI AflII AflIII AleI AloI AlwNI ApoI AvaI BanI BanII BbeI BbsI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PpiI PpuMI PsiI PstI PvuII SacI SacII SfcI SfoI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BmgBI BmtI BsaBI BsiEI BsiWI BspEI BspMI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI