(Circular) (Six-base) MAPSORT of: pFN21AB7034.seq Check: 6208 from: 1 to: 5980 pFN21AB7034 5980 bp With 182 enzymes: SgfI * November 11, 2016 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3150 3323 1937 Size: 1213 173 4594 Fragments arranged by size: 4594 1213 173 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2026 4043 5427 5695 1632 Size: 394 2017 1384 268 1917 Fragments arranged by size: 2017 1917 1384 394 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 2559 4879 5856 1203 Size: 1356 2320 977 1327 Fragments arranged by size: 2320 1356 1327 977 AgeI A'CCGG_T Cuts at: 2601 4811 2601 Size: 2210 3770 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 2336 1166 Size: 1170 4810 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 2301 2954 3047 3723 5295 2301 Size: 653 93 676 1572 2986 Fragments arranged by size: 2986 1572 676 653 93 ApaI G_GGCC'C Cuts at: 2083 2083 Size: 5980 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 3289 3515 3667 5776 1493 Size: 1796 226 152 2109 1697 Fragments arranged by size: 2109 1796 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2744 2931 3305 1870 Size: 73 133 668 187 374 4545 Fragments arranged by size: 4545 668 374 187 133 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2740 3301 4756 618 Size: 325 217 627 953 561 1455 1842 Fragments arranged by size: 1842 1455 953 627 561 325 217 BanII G_rGCy'C Cuts at: 1391 2083 2751 3299 1391 Size: 692 668 548 4072 Fragments arranged by size: 4072 692 668 548 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2827 928 Size: 971 928 4081 Fragments arranged by size: 4081 971 928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2631 3223 3872 5082 2631 Size: 592 649 1210 3529 Fragments arranged by size: 3529 1210 649 592 BclI T'GATC_A Cuts at: 1548 2853 1548 Size: 1305 4675 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 2684 4591 Size: 107 122 71 631 605 345 667 1907 Cuts at: 4591 136 Size: 1525 Fragments arranged by size: 1907 1525 667 631 605 345 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 2083 2745 4041 5197 1790 Size: 293 662 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 662 293 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4640 1239 Size: 3401 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2705 4105 4970 5268 2705 Size: 1400 865 298 3417 Fragments arranged by size: 3417 1400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2282 2333 2601 4413 4811 5085 5232 5643 2282 Size: 51 268 1812 398 274 147 411 2619 Fragments arranged by size: 2619 1812 411 398 274 268 147 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2328 2358 1178 Size: 30 165 30 925 30 4800 Fragments arranged by size: 4800 925 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2708 3056 1186 Size: 1009 513 348 4110 Fragments arranged by size: 4110 1009 513 348 BseYI C'CCAG_C Cuts at: 3185 5183 5679 3185 Size: 1998 496 3486 Fragments arranged by size: 3486 1998 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 1163 Size: 573 678 4729 Fragments arranged by size: 4729 678 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2469 2778 4041 4126 5197 2469 Size: 309 1263 85 1071 3252 Fragments arranged by size: 3252 1263 1071 309 85 BsmI GAATG_Cn' Cuts at: 2261 3491 3584 2261 Size: 1230 93 4657 Fragments arranged by size: 4657 1230 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2469 2745 2751 2778 3299 4041 Size: 399 293 386 276 6 27 521 742 Cuts at: 4041 4126 5197 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 521 399 386 293 276 85 27 6 BspEI T'CCGG_A Cuts at: 2282 5643 2282 Size: 3361 2619 BspHI T'CATG_A Cuts at: 2580 2767 3869 5599 2580 Size: 187 1102 1730 2961 Fragments arranged by size: 2961 1730 1102 187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3242 3335 844 Size: 2398 93 3489 Fragments arranged by size: 3489 2398 93 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2410 2601 4624 4811 1700 Size: 110 600 191 2023 187 2869 Fragments arranged by size: 2869 2023 600 191 187 110 BsrGI T'GTAC_A Cuts at: 96 2328 2421 96 Size: 2232 93 3655 Fragments arranged by size: 3655 2232 93 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 2108 3093 794 Size: 1314 985 3681 Fragments arranged by size: 3681 1314 985 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2119 3310 3677 4062 4079 5531 5617 5629 Size: 147 1191 367 385 17 1452 86 12 Cuts at: 5629 5975 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2439 2862 513 Size: 552 939 8 427 423 3631 Fragments arranged by size: 3631 939 552 427 423 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2680 487 Size: 683 56 1454 3787 Fragments arranged by size: 3787 1454 683 56 BtsI GCAGTG_nn' Cuts at: 2506 2538 3208 3496 4290 4310 2506 Size: 32 670 288 794 20 4176 Fragments arranged by size: 4176 794 670 288 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2370 2436 Size: 54 1425 50 275 18 201 339 66 Cuts at: 2436 2676 2691 3427 4316 5945 8 Size: 240 15 736 889 1629 43 Fragments arranged by size: 1629 1425 889 736 339 275 240 201 66 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2247 2905 3910 1353 Size: 894 658 1005 3423 Fragments arranged by size: 3423 1005 894 658 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2026 4043 4640 5427 5695 1239 Size: 393 394 2017 597 787 268 1524 Fragments arranged by size: 2017 1524 787 597 394 393 268 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 5127 1678 Size: 3449 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 3221 3253 4621 Size: 32 135 32 539 32 1078 32 1368 Cuts at: 4621 4653 4695 4727 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1368 1078 539 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2202 3276 3324 3570 669 Size: 988 281 264 1074 48 246 3079 Fragments arranged by size: 3079 1074 988 281 264 246 48 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 2202 3276 3570 2202 Size: 1074 294 4612 Fragments arranged by size: 4612 1074 294 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5094 5278 1305 Size: 3789 184 2007 Fragments arranged by size: 3789 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 2438 2678 2693 10 Size: 54 1425 343 540 66 240 15 3297 Fragments arranged by size: 3297 1425 540 343 240 66 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2378 2738 3938 4297 4456 518 Size: 648 1170 42 360 1200 359 159 2042 Fragments arranged by size: 2042 1200 1170 648 360 359 159 42 MspA1I CmG'CkG Cuts at: 1606 2014 2059 2851 2951 3396 4073 5221 5466 Size: 408 45 792 100 445 677 1148 245 Cuts at: 5466 1606 Size: 2120 Fragments arranged by size: 2120 1148 792 677 445 408 245 100 45 NaeI GCC'GGC Cuts at: 2412 2412 Size: 5980 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NgoMIV G'CCGG_C Cuts at: 2410 2410 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2428 2563 3338 4883 1132 Size: 97 1199 135 775 1545 2229 Fragments arranged by size: 2229 1545 1199 775 135 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 2559 4879 2559 Size: 2320 3660 PflMI CCAn_nnn'nTGG Cuts at: 1108 2301 1108 Size: 1193 4787 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3195 3550 3195 Size: 355 5625 PspOMI G'GGCC_C Cuts at: 2079 2079 Size: 5980 PstI C_TGCA'G Cuts at: 830 2545 3332 830 Size: 1715 787 3478 Fragments arranged by size: 3478 1715 787 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2951 2951 Size: 5980 SacII CC_GC'GG Cuts at: 2015 2015 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SapI GCTCTTCn'nnn_ Cuts at: 2905 2905 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2541 3151 3328 4463 5144 5335 826 Size: 219 1496 610 177 1135 681 191 1471 Fragments arranged by size: 1496 1471 1135 681 610 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 2078 2746 2933 3307 1872 Size: 206 668 187 374 4545 Fragments arranged by size: 4545 668 374 206 187 SmlI C'TyrA_G Cuts at: 820 1017 1943 2720 4084 4985 5247 820 Size: 197 926 777 1364 901 262 1553 Fragments arranged by size: 1553 1364 926 901 777 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SrfI GCCC'GGGC Cuts at: 2746 2746 Size: 5980 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 3419 513 Size: 552 939 1415 3074 Fragments arranged by size: 3074 1415 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4007 4024 4177 4362 4807 325 Size: 1255 2427 17 153 185 445 1498 Fragments arranged by size: 2427 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2328 2421 4226 Size: 274 80 33 51 1443 351 93 1805 Cuts at: 4226 5938 96 Size: 1712 138 Fragments arranged by size: 1805 1712 1443 351 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2104 2136 2301 2333 5940 5972 2104 Size: 32 165 32 3607 32 2112 Fragments arranged by size: 3607 2112 165 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2686 2686 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 2076 2744 2931 3305 1870 Size: 206 668 187 374 4545 Fragments arranged by size: 4545 668 374 206 187 XmnI GAAnn'nnTTC Cuts at: 2659 4109 2659 Size: 1450 4530 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SwaI