(Circular) (Six-base) MAPSORT of: pFN21AE3668.seq Check: 6948 from: 1 to: 6100 pFN21AE3668 6100 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3004 3004 Size: 6100 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 3443 1937 Size: 1191 315 4594 Fragments arranged by size: 4594 1191 315 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2239 4163 5547 5815 1632 Size: 482 125 1924 1384 268 1917 Fragments arranged by size: 1924 1917 1384 482 268 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 3144 4999 5976 1203 Size: 1941 1855 977 1327 Fragments arranged by size: 1941 1855 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 2186 1166 Size: 1020 5080 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 2062 2866 3843 5415 2062 Size: 804 977 1572 2747 Fragments arranged by size: 2747 1572 977 804 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2336 3425 1870 Size: 73 393 1089 4545 Fragments arranged by size: 4545 1089 393 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2777 3421 4876 618 Size: 325 217 627 990 644 1455 1842 Fragments arranged by size: 1842 1455 990 644 627 325 217 BanII G_rGCy'C Cuts at: 1391 2846 3419 1391 Size: 1455 573 4072 Fragments arranged by size: 4072 1455 573 BbeI G_GCGC'C Cuts at: 2781 2781 Size: 6100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 2481 2658 928 Size: 971 416 166 177 4370 Fragments arranged by size: 4370 971 416 177 166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2828 2862 4291 4325 1903 Size: 34 50 34 807 34 1429 34 3678 Fragments arranged by size: 3678 1429 807 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4711 136 Size: 107 122 71 631 605 3039 1525 Fragments arranged by size: 3039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3045 6095 3045 Size: 3050 3050 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 2687 4161 5317 1790 Size: 897 1474 1156 2573 Fragments arranged by size: 2573 1474 1156 897 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3831 4789 442 Size: 416 53 83 399 24 2414 958 1753 Fragments arranged by size: 2414 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2837 4760 1239 Size: 926 672 1923 2579 Fragments arranged by size: 2579 1923 926 672 Bpu10I CC'TnA_GC Cuts at: 2924 3059 2924 Size: 135 5965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2141 3364 4763 882 Size: 617 642 1223 1399 2219 Fragments arranged by size: 2219 1399 1223 642 617 BsaAI yAC'GTr Cuts at: 493 1204 2188 3891 493 Size: 711 984 1703 2702 Fragments arranged by size: 2702 1703 984 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2343 2778 3168 3296 Size: 53 83 186 936 810 435 390 128 Cuts at: 3296 3907 4289 4924 275 Size: 611 382 635 1451 Fragments arranged by size: 1451 936 810 635 611 435 390 382 186 128 83 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2177 2207 2213 2243 2282 Size: 30 165 30 774 30 6 30 39 Cuts at: 2282 2312 1178 Size: 30 4966 Fragments arranged by size: 4966 774 165 39 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2303 2726 3344 1186 Size: 913 204 423 618 3942 Fragments arranged by size: 3942 913 618 423 204 BseYI C'CCAG_C Cuts at: 3067 3254 3299 3391 5303 5799 3067 Size: 187 45 92 1912 496 3368 Fragments arranged by size: 3368 1912 496 187 92 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3233 1163 Size: 573 1497 4030 Fragments arranged by size: 4030 1497 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3550 4311 5339 1540 Size: 275 186 1036 513 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 513 275 186 BsiHKAI G_wGCw'C Cuts at: 2846 3065 3152 4161 4246 5317 2846 Size: 219 87 1009 85 1071 3629 Fragments arranged by size: 3629 1071 1009 219 87 85 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 BsmBI CGTCTCn'nnnn_ Cuts at: 2101 2556 2990 2101 Size: 455 434 5211 Fragments arranged by size: 5211 455 434 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2687 2846 3065 3152 3419 4161 4246 Size: 399 897 159 219 87 267 742 85 Cuts at: 4246 5317 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 897 742 399 267 219 159 87 85 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2952 3004 3455 844 Size: 2108 52 451 3489 Fragments arranged by size: 3489 2108 451 52 BsrBI CCG'CTC Cuts at: 1394 1823 3268 3987 1394 Size: 429 1445 719 3507 Fragments arranged by size: 3507 1445 719 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2613 2701 4595 4769 65 Size: 1183 1365 88 1894 174 1396 Fragments arranged by size: 1894 1396 1365 1183 174 88 BsrFI r'CCGG_y Cuts at: 1700 1810 2351 4744 4931 1700 Size: 110 541 2393 187 2869 Fragments arranged by size: 2869 2393 541 187 110 BsrGI T'GTAC_A Cuts at: 96 3240 96 Size: 3144 2956 BssHII G'CGCG_C Cuts at: 1925 3133 1925 Size: 1208 4892 BssSI C'ACGA_G Cuts at: 2930 4160 5172 2930 Size: 1230 1012 3858 Fragments arranged by size: 3858 1230 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2691 2700 794 Size: 1897 9 4194 Fragments arranged by size: 4194 1897 9 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstYI r'GATC_y Cuts at: 1972 2058 3045 3430 3797 4182 4199 5651 5737 Size: 86 987 385 367 385 17 1452 86 Cuts at: 5737 5749 6095 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 987 385 385 367 346 86 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2138 2524 2138 Size: 386 5714 BtgI C'CryG_G Cuts at: 513 1065 2131 2953 513 Size: 552 1066 822 3660 Fragments arranged by size: 3660 1066 822 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 2303 2355 3343 3616 4410 4430 2303 Size: 52 988 273 794 20 3973 Fragments arranged by size: 3973 988 794 273 52 20 ClaI AT'CG_AT Cuts at: 1554 2481 1554 Size: 927 5173 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2625 3340 3547 Size: 54 1425 50 275 18 795 715 207 Cuts at: 3547 4436 6065 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 795 715 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 2375 2954 4030 1353 Size: 798 224 579 1076 3423 Fragments arranged by size: 3423 1076 798 579 224 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2844 2844 Size: 6100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2165 2239 2837 4163 4760 5547 Size: 393 482 51 74 598 1326 597 787 Cuts at: 5547 5815 1239 Size: 268 1524 Fragments arranged by size: 1524 1326 787 598 597 482 393 268 74 51 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2096 Size: 6100 EcoO109I rG'GnC_Cy Cuts at: 2089 2089 Size: 6100 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FspI TGC'GCA Cuts at: 2978 3141 3212 3287 4606 2978 Size: 163 71 75 1319 4472 Fragments arranged by size: 4472 1319 163 75 71 FspAI rTGC'GCAy Cuts at: 3212 3212 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2781 5247 1678 Size: 1103 2466 2531 Fragments arranged by size: 2531 2466 1103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2366 2398 2553 Size: 32 135 32 641 32 121 32 155 Cuts at: 2553 2585 2828 2860 3110 3142 4741 4773 4815 Size: 32 243 32 250 32 1599 32 42 Cuts at: 4815 4847 1373 Size: 32 2626 Fragments arranged by size: 2626 1599 641 250 243 155 135 121 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KasI G'GCGC_C Cuts at: 2777 2777 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 3306 5214 5398 1305 Size: 1116 885 1908 184 2007 Fragments arranged by size: 2007 1908 1116 885 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2627 10 Size: 54 1425 343 795 3483 Fragments arranged by size: 3483 1425 795 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2678 3218 4058 4417 4576 518 Size: 648 1020 492 540 840 359 159 2042 Fragments arranged by size: 2042 1020 840 648 540 492 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2133 2866 2974 3303 3516 4193 5341 Size: 471 56 733 108 329 213 677 1148 Cuts at: 5341 5586 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 733 677 471 329 245 213 108 56 NarI GG'CG_CC Cuts at: 2778 2778 Size: 6100 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5548 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2758 3148 3184 3458 5003 1132 Size: 97 1529 390 36 274 1545 2229 Fragments arranged by size: 2229 1545 1529 390 274 97 36 PasI CC'CwG_GG Cuts at: 1348 2440 1348 Size: 1092 5008 PciI A'CATG_T Cuts at: 3144 4999 3144 Size: 1855 4245 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PfoI T'CCnGG_A Cuts at: 2403 2403 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2089 Size: 6100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PstI C_TGCA'G Cuts at: 830 2082 3452 830 Size: 1252 1370 3478 Fragments arranged by size: 3478 1370 1252 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2077 2866 2974 3303 2077 Size: 789 108 329 4874 Fragments arranged by size: 4874 789 329 108 SacI G_AGCT'C Cuts at: 2846 2846 Size: 6100 SacII CC_GC'GG Cuts at: 2134 2134 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SapI GCTCTTCn'nnn_ Cuts at: 2375 2954 2375 Size: 579 5521 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2078 3448 4583 5264 5455 826 Size: 219 1033 1370 1135 681 191 1471 Fragments arranged by size: 1471 1370 1135 1033 681 219 191 SfoI GGC'GCC Cuts at: 2779 2779 Size: 6100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4204 5105 5367 820 Size: 197 926 2261 901 262 1553 Fragments arranged by size: 2261 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3184 3458 1132 Size: 2052 274 3774 Fragments arranged by size: 3774 2052 274 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 2522 2963 3383 1517 Size: 1005 441 420 4234 Fragments arranged by size: 4234 1005 441 420 StyI C'CwwG_G Cuts at: 513 1065 2508 3539 513 Size: 552 1443 1031 3074 Fragments arranged by size: 3074 1443 1031 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 4127 4144 4297 4482 4927 325 Size: 1255 651 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 2892 3240 Size: 274 80 33 51 1443 348 567 348 Cuts at: 3240 4346 6058 96 Size: 1106 1712 138 Fragments arranged by size: 1712 1443 1106 567 348 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2282 2314 6060 6092 2282 Size: 32 3746 32 2290 Fragments arranged by size: 3746 2290 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI