(Linear) (Six-base) MAPSORT of: KIEE4522.seq Check: 5209 from: 1 to: 1686 KIEE4522.orf With 182 enzymes: SgfI * March 22, 2012 18:16 .. AccI GT'mk_AC Cuts at: 0 494 1686 Size: 494 1192 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 902 936 1686 Size: 673 229 34 750 Fragments arranged by size: 750 673 229 34 AgeI A'CCGG_T Cuts at: 0 1141 1686 Size: 1141 545 AhdI GACnn_n'nnGTC Cuts at: 0 347 1686 Size: 347 1339 AleI CACnn'nnGTG Cuts at: 0 169 1157 1686 Size: 169 988 529 Fragments arranged by size: 988 529 169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 765 1686 Size: 765 921 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 175 207 523 555 1686 Size: 175 32 316 32 1131 Fragments arranged by size: 1131 316 175 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 925 1686 Size: 925 761 ApaI G_GGCC'C Cuts at: 0 367 1686 Size: 367 1319 ApaLI G'TGCA_C Cuts at: 0 617 1686 Size: 617 1069 ApoI r'AATT_y Cuts at: 0 388 437 1089 1266 1686 Size: 388 49 652 177 420 Fragments arranged by size: 652 420 388 177 49 BanI G'GyrC_C Cuts at: 0 724 1686 Size: 724 962 BanII G_rGCy'C Cuts at: 0 367 1686 Size: 367 1319 BbsI GAAGACnn'nnnn_ Cuts at: 0 1494 1686 Size: 1494 192 BbvCI CC'TCA_GC Cuts at: 0 1304 1686 Size: 1304 382 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 45 79 1686 Size: 45 34 1607 Fragments arranged by size: 1607 45 34 BciVI GTATCCnnnnn_n' Cuts at: 0 306 1686 Size: 306 1380 BfrBI ATG'CAT Cuts at: 0 675 1686 Size: 675 1011 BglII A'GATC_T Cuts at: 0 656 1686 Size: 656 1030 Bme1580I G_kGCm'C Cuts at: 0 55 367 621 729 1686 Size: 55 312 254 108 957 Fragments arranged by size: 957 312 254 108 55 BmrI ACTGGGnnnn_n' Cuts at: 0 986 1429 1529 1686 Size: 986 443 100 157 Fragments arranged by size: 986 443 157 100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1034 1686 Size: 1034 652 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 401 781 1686 Size: 401 380 905 Fragments arranged by size: 905 401 380 Bpu10I CC'TnA_GC Cuts at: 0 1304 1686 Size: 1304 382 BsaI GGTCTCn'nnnn_ Cuts at: 0 814 1054 1686 Size: 814 240 632 Fragments arranged by size: 814 632 240 BsaBI GATnn'nnATC Cuts at: 0 872 1686 Size: 872 814 BsaWI w'CCGG_w Cuts at: 0 1141 1686 Size: 1141 545 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1141 1306 1686 Size: 1141 165 380 Fragments arranged by size: 1141 380 165 BseYI C'CCAG_C Cuts at: 0 1640 1686 Size: 1640 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1652 1686 Size: 1652 34 BsiEI CG_ry'CG Cuts at: 0 189 252 1686 Size: 189 63 1434 Fragments arranged by size: 1434 189 63 BsiHKAI G_wGCw'C Cuts at: 0 621 1020 1686 Size: 621 399 666 Fragments arranged by size: 666 621 399 BsmI GAATG_Cn' Cuts at: 0 956 1686 Size: 956 730 Bsp1286I G_dGCh'C Cuts at: 0 55 367 621 729 1020 1686 Size: 55 312 254 108 291 666 Fragments arranged by size: 666 312 291 254 108 55 BsrBI CCG'CTC Cuts at: 0 59 1514 1686 Size: 59 1455 172 Fragments arranged by size: 1455 172 59 BsrDI GCAATG_nn' Cuts at: 0 935 1622 1686 Size: 935 687 64 Fragments arranged by size: 935 687 64 BsrFI r'CCGG_y Cuts at: 0 144 188 1141 1686 Size: 144 44 953 545 Fragments arranged by size: 953 545 144 44 BsrGI T'GTAC_A Cuts at: 0 1547 1686 Size: 1547 139 BssSI C'ACGA_G Cuts at: 0 54 1008 1686 Size: 54 954 678 Fragments arranged by size: 954 678 54 BstXI CCAn_nnnn'nTGG Cuts at: 0 171 976 1073 1686 Size: 171 805 97 613 Fragments arranged by size: 805 613 171 97 BstYI r'GATC_y Cuts at: 0 656 704 1686 Size: 656 48 982 Fragments arranged by size: 982 656 48 BtgI C'CryG_G Cuts at: 0 137 984 1686 Size: 137 847 702 Fragments arranged by size: 847 702 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 204 213 1686 Size: 204 9 1473 Fragments arranged by size: 1473 204 9 BtsI GCAGTG_nn' Cuts at: 0 1664 1686 Size: 1664 22 ClaI AT'CG_AT Cuts at: 0 1553 1686 Size: 1553 133 DrdI GACnn_nn'nnGTC Cuts at: 0 828 1597 1686 Size: 828 769 89 Fragments arranged by size: 828 769 89 EaeI y'GGCC_r Cuts at: 0 186 1260 1686 Size: 186 1074 426 Fragments arranged by size: 1074 426 186 EagI C'GGCC_G Cuts at: 0 186 1686 Size: 186 1500 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 401 673 781 902 936 1686 Size: 401 272 108 121 34 750 Fragments arranged by size: 750 401 272 121 108 34 EcoO109I rG'GnC_Cy Cuts at: 0 363 364 409 1338 1590 1686 Size: 363 1 45 929 252 96 Fragments arranged by size: 929 363 252 96 45 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 514 649 1686 Size: 514 135 1037 Fragments arranged by size: 1037 514 135 HaeII r_GCGC'y Cuts at: 0 98 1686 Size: 98 1588 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 333 365 641 673 1335 1367 1686 Size: 333 32 276 32 662 32 319 Fragments arranged by size: 662 333 319 276 32 32 32 HincII GTy'rAC Cuts at: 0 747 1686 Size: 747 939 HindIII A'AGCT_T Cuts at: 0 578 1686 Size: 578 1108 HpaI GTT'AAC Cuts at: 0 747 1686 Size: 747 939 MfeI C'AATT_G Cuts at: 0 458 1686 Size: 458 1228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1530 1686 Size: 1530 156 MslI CAynn'nnrTG Cuts at: 0 169 1157 1686 Size: 169 988 529 Fragments arranged by size: 988 529 169 MspA1I CmG'CkG Cuts at: 0 153 808 986 1644 1686 Size: 153 655 178 658 42 Fragments arranged by size: 658 655 178 153 42 NaeI GCC'GGC Cuts at: 0 146 1686 Size: 146 1540 NgoMIV G'CCGG_C Cuts at: 0 144 1686 Size: 144 1542 NsiI A_TGCA'T Cuts at: 0 677 1686 Size: 677 1009 NspI r_CATG'y Cuts at: 0 1005 1686 Size: 1005 681 PasI CC'CwG_GG Cuts at: 0 358 1686 Size: 358 1328 PflMI CCAn_nnn'nTGG Cuts at: 0 1441 1686 Size: 1441 245 PpuMI rG'GwC_Cy Cuts at: 0 409 1590 1686 Size: 409 1181 96 Fragments arranged by size: 1181 409 96 PshAI GACnn'nnGTC Cuts at: 0 979 1686 Size: 979 707 PspOMI G'GGCC_C Cuts at: 0 363 1686 Size: 363 1323 PstI C_TGCA'G Cuts at: 0 1649 1686 Size: 1649 37 PvuII CAG'CTG Cuts at: 0 808 1644 1686 Size: 808 836 42 Fragments arranged by size: 836 808 42 SacII CC_GC'GG Cuts at: 0 987 1686 Size: 987 699 SexAI A'CCwGG_T Cuts at: 0 812 1276 1686 Size: 812 464 410 Fragments arranged by size: 812 464 410 SfcI C'TryA_G Cuts at: 0 1645 1686 Size: 1645 41 SphI G_CATG'C Cuts at: 0 1005 1686 Size: 1005 681 SspI AAT'ATT Cuts at: 0 396 1686 Size: 396 1290 StyI C'CwwG_G Cuts at: 0 498 1686 Size: 498 1188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 238 1686 Size: 238 1448 TatI w'GTAC_w Cuts at: 0 1547 1686 Size: 1547 139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 171 1686 Size: 171 1515 XmnI GAAnn'nnTTC Cuts at: 0 82 1270 1372 1449 1686 Size: 82 1188 102 77 237 Fragments arranged by size: 1188 237 102 82 77 Enzymes that do cut: AccI AcuI AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EagI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MslI MspA1I NaeI NgoMIV NsiI NspI PasI PflMI PpuMI PshAI PspOMI PstI PvuII SacII SexAI SfcI SphI SspI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BclI BglI BlpI BmgBI BmtI BpuEI BsaAI BsaHI BsaXI BsiWI BsmBI BspEI BspHI BspMI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI KasI KpnI MluI MscI NarI NcoI NdeI NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI