(Circular) (Six-base) MAPSORT of: pFN21AB8616.seq Check: 5427 from: 1 to: 5809 pFN21AB8616 5809 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2407 3619 278 Size: 53 83 186 936 871 1212 2468 Fragments arranged by size: 2468 1212 936 871 186 83 53 AccI GT'mk_AC Cuts at: 1937 3152 1937 Size: 1215 4594 Acc65I G'GTAC_C Cuts at: 2488 3130 2488 Size: 642 5167 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 2523 2924 3036 3872 5256 5524 1632 Size: 786 105 401 112 836 1384 268 1917 Fragments arranged by size: 1917 1384 836 786 401 268 112 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 3091 4538 3091 Size: 1447 4362 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AlwNI CAG_nnn'CTG Cuts at: 3552 5124 3552 Size: 1572 4237 ApaI G_GGCC'C Cuts at: 2465 2465 Size: 5809 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 2120 2305 2747 3104 3118 3344 3496 5605 Size: 627 185 442 357 14 226 152 2109 Cuts at: 5605 1493 Size: 1697 Fragments arranged by size: 2109 1697 627 442 357 226 185 152 14 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 2042 3134 1870 Size: 73 99 1092 4545 Fragments arranged by size: 4545 1092 99 73 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2488 3130 4585 618 Size: 325 217 627 701 642 1455 1842 Fragments arranged by size: 1842 1455 701 642 627 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2465 3128 1391 Size: 643 431 663 4072 Fragments arranged by size: 4072 663 643 431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3042 928 Size: 971 1143 3695 Fragments arranged by size: 3695 1143 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4911 3701 Size: 1210 4599 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4420 136 Size: 107 122 71 631 605 2748 1525 Fragments arranged by size: 2748 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2154 5804 2154 Size: 3650 2159 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 2465 2736 3870 5026 1790 Size: 675 271 1134 1156 2573 Fragments arranged by size: 2573 1156 1134 675 271 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2723 3540 4498 Size: 416 53 83 399 24 1306 817 958 Cuts at: 4498 442 Size: 1753 Fragments arranged by size: 1753 1306 958 817 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2829 2829 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4469 1239 Size: 3230 2579 Bpu10I CC'TnA_GC Cuts at: 2255 2255 Size: 5809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3934 4799 5097 3934 Size: 865 298 4646 Fragments arranged by size: 4646 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2817 4472 882 Size: 617 1318 1655 2219 Fragments arranged by size: 2219 1655 1318 617 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 5803 5803 Size: 5809 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2404 3616 3998 4633 Size: 53 83 186 936 871 1212 382 635 Cuts at: 4633 275 Size: 1451 Fragments arranged by size: 1451 1212 936 871 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4242 4640 4914 5061 5472 4242 Size: 398 274 147 411 4579 Fragments arranged by size: 4579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5584 Fragments arranged by size: 5584 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2981 1186 Size: 1795 4014 BseYI C'CCAG_C Cuts at: 2464 2736 5012 5508 2464 Size: 272 2276 496 2765 Fragments arranged by size: 2765 2276 496 272 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3259 4020 5048 1540 Size: 275 186 1258 761 1028 2301 Fragments arranged by size: 2301 1258 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 3870 3955 5026 2012 Size: 1858 85 1071 2795 Fragments arranged by size: 2795 1858 1071 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2034 2465 2736 3128 3870 3955 Size: 399 222 22 431 271 392 742 85 Cuts at: 3955 5026 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 431 399 392 271 222 85 22 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3164 844 Size: 2320 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2833 3696 1394 Size: 429 1010 863 3507 Fragments arranged by size: 3507 1010 863 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2187 2604 2794 4304 4478 65 Size: 1183 939 417 190 1510 174 1396 Fragments arranged by size: 1510 1396 1183 939 417 190 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4453 4640 1700 Size: 110 2643 187 2869 Fragments arranged by size: 2869 2643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 3869 4881 3869 Size: 1012 4797 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5809 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstXI CCAn_nnnn'nTGG Cuts at: 2399 2807 2399 Size: 408 5401 BstYI r'GATC_y Cuts at: 1972 2154 3139 3506 3891 3908 5360 5446 5458 Size: 182 985 367 385 17 1452 86 12 Cuts at: 5458 5804 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 985 385 367 346 182 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2020 2522 513 Size: 552 955 502 3800 Fragments arranged by size: 3800 955 552 502 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 3325 4119 4139 3325 Size: 794 20 4995 Fragments arranged by size: 4995 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 2752 3113 2752 Size: 361 5448 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2403 4816 809 Size: 507 1087 2413 1802 Fragments arranged by size: 2413 1802 1087 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3256 4145 5774 Size: 54 1425 50 275 18 1426 889 1629 Cuts at: 5774 8 Size: 43 Fragments arranged by size: 1629 1426 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 1537 Size: 275 1444 4090 Fragments arranged by size: 4090 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2421 2798 2823 3739 1353 Size: 1068 377 25 916 3423 Fragments arranged by size: 3423 1068 916 377 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4396 4770 4916 387 Size: 1064 138 2807 374 146 1280 Fragments arranged by size: 2807 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2418 2523 2924 3036 3872 4469 5256 Size: 393 786 105 401 112 836 597 787 Cuts at: 5256 5524 1239 Size: 268 1524 Fragments arranged by size: 1524 836 787 786 597 401 393 268 112 105 EcoO109I rG'GnC_Cy Cuts at: 2461 2461 Size: 5809 EcoRI G'AATT_C Cuts at: 2305 3104 3118 3496 2305 Size: 799 14 378 4618 Fragments arranged by size: 4618 799 378 14 FspI TGC'GCA Cuts at: 2111 4315 2111 Size: 2204 3605 HaeII r_GCGC'y Cuts at: 1678 2095 4956 1678 Size: 417 2861 2531 Fragments arranged by size: 2861 2531 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 3019 3051 3077 Size: 32 135 32 743 32 672 32 26 Cuts at: 3077 3080 3109 3112 4450 4482 4524 4556 1373 Size: 3 29 3 1338 32 42 32 2626 Fragments arranged by size: 2626 1338 743 672 135 42 32 32 32 32 32 32 29 26 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3153 3399 669 Size: 988 281 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KpnI G_GTAC'C Cuts at: 2492 3134 2492 Size: 642 5167 MfeI C'AATT_G Cuts at: 2268 3408 2268 Size: 1140 4669 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4923 5107 1305 Size: 3618 184 2007 Fragments arranged by size: 3618 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3987 Fragments arranged by size: 3987 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2702 2859 3767 4126 4285 518 Size: 648 1536 157 908 359 159 2042 Fragments arranged by size: 2042 1536 908 648 359 159 157 MspA1I CmG'CkG Cuts at: 1606 2022 2059 2524 2740 2911 3225 3902 5050 Size: 416 37 465 216 171 314 677 1148 Cuts at: 5050 5295 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 465 416 314 245 216 171 37 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5257 NdeI CA'TA_TG Cuts at: 387 2898 387 Size: 2511 3298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 3167 4712 1132 Size: 97 1938 1545 2229 Fragments arranged by size: 2229 1938 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5809 PfoI T'CCnGG_A Cuts at: 2341 2341 Size: 5809 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3033 3379 3033 Size: 346 5463 PspOMI G'GGCC_C Cuts at: 2461 2461 Size: 5809 PstI C_TGCA'G Cuts at: 830 3161 830 Size: 2331 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 PvuII CAG'CTG Cuts at: 2740 2911 2740 Size: 171 5638 SacII CC_GC'GG Cuts at: 2023 2525 2023 Size: 502 5307 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SapI GCTCTTCn'nnn_ Cuts at: 2823 2823 Size: 5809 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 2647 4057 5769 2647 Size: 1410 1712 2687 Fragments arranged by size: 2687 1712 1410 SfcI C'TryA_G Cuts at: 826 1045 2355 3157 4292 4973 5164 826 Size: 219 1310 802 1135 681 191 1471 Fragments arranged by size: 1471 1310 1135 802 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 3136 1872 Size: 1264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3913 4814 5076 820 Size: 197 926 1970 901 262 1553 Fragments arranged by size: 1970 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 2335 152 Size: 2183 3626 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3016 3733 5 Size: 47 2964 717 2081 Fragments arranged by size: 2964 2081 717 47 StuI AGG'CCT Cuts at: 1517 2664 1517 Size: 1147 4662 StyI C'CwwG_G Cuts at: 513 1065 2614 2931 3248 513 Size: 552 1549 317 317 3074 Fragments arranged by size: 3074 1549 552 317 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3836 3853 4006 4191 4636 325 Size: 1255 2256 17 153 185 445 1498 Fragments arranged by size: 2256 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 2645 4055 Size: 274 80 33 51 1443 468 200 1410 Cuts at: 4055 5767 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1410 468 274 200 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5769 5801 5769 Size: 32 5777 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 3134 1870 Size: 1264 4545 XmnI GAAnn'nnTTC Cuts at: 3938 3938 Size: 5809 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2405 3617 276 Size: 53 83 186 936 871 1212 2468 Fragments arranged by size: 2468 1212 936 871 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI