(Circular) (Six-base) MAPSORT of: pFN21AB6557.seq Check: 380 from: 1 to: 5821 pFN21AB6557 5821 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2301 2301 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2064 3164 1937 Size: 127 1100 4594 Fragments arranged by size: 4594 1100 127 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3884 5268 5536 1632 Size: 2252 1384 268 1917 Fragments arranged by size: 2252 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 2710 4550 2710 Size: 1840 3981 AleI CACnn'nnGTG Cuts at: 1166 2606 1166 Size: 1440 4381 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 3564 5136 3564 Size: 1572 4249 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 3146 1870 Size: 73 1203 4545 Fragments arranged by size: 4545 1203 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2289 2983 3046 3142 Size: 325 217 627 305 197 694 63 96 Cuts at: 3142 4597 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 694 627 325 305 217 197 96 63 BanII G_rGCy'C Cuts at: 1391 2123 3140 1391 Size: 732 1017 4072 Fragments arranged by size: 4072 1017 732 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2256 4432 136 Size: 107 122 71 631 605 584 2176 1525 Fragments arranged by size: 2176 1525 631 605 584 122 107 71 BglII A'GATC_T Cuts at: 3003 5816 3003 Size: 2813 3008 BlpI GC'TnA_GC Cuts at: 2345 3238 2345 Size: 893 4928 Bme1580I G_kGCm'C Cuts at: 1790 2652 3882 5038 1790 Size: 862 1230 1156 2573 Fragments arranged by size: 2573 1230 1156 862 BmgBI CAC'GTC Cuts at: 1318 2128 2172 2679 1318 Size: 810 44 507 4460 Fragments arranged by size: 4460 810 507 44 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2164 2255 3552 Size: 416 53 83 399 24 747 91 1297 Cuts at: 3552 4510 442 Size: 958 1753 Fragments arranged by size: 1753 1297 958 747 416 399 91 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2607 2690 2902 4481 1239 Size: 1368 83 212 1579 2579 Fragments arranged by size: 2579 1579 1368 212 83 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 2708 3946 4811 5109 2468 Size: 240 1238 865 298 3180 Fragments arranged by size: 3180 1238 865 298 240 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2545 2948 4484 882 Size: 617 1046 403 1536 2219 Fragments arranged by size: 2219 1536 1046 617 403 BsaAI yAC'GTr Cuts at: 493 1204 3612 493 Size: 711 2408 2702 Fragments arranged by size: 2702 2408 711 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3628 4010 4645 275 Size: 53 83 186 936 2095 382 635 1451 Fragments arranged by size: 2095 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2267 2297 2504 2525 2534 Size: 30 165 30 864 30 207 21 9 Cuts at: 2534 2555 2663 2693 1178 Size: 21 108 30 4306 Fragments arranged by size: 4306 864 207 165 108 30 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 2476 2651 3022 5024 5520 2476 Size: 175 371 2002 496 2777 Fragments arranged by size: 2777 2002 496 371 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 4861 Fragments arranged by size: 4861 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 2346 3882 3967 5038 2232 Size: 114 1536 85 1071 3015 Fragments arranged by size: 3015 1536 1071 114 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2232 2346 2652 3140 3882 3967 Size: 399 333 109 114 306 488 742 85 Cuts at: 3967 5038 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 488 399 333 306 114 109 85 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 2301 3176 844 Size: 1370 87 875 3489 Fragments arranged by size: 3489 1370 875 87 BsrBI CCG'CTC Cuts at: 1394 1823 2355 3708 1394 Size: 429 532 1353 3507 Fragments arranged by size: 3507 1353 532 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2563 4316 4490 65 Size: 1183 1315 1753 174 1396 Fragments arranged by size: 1753 1396 1315 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2664 4465 4652 1700 Size: 110 854 1801 187 2869 Fragments arranged by size: 2869 1801 854 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2980 3076 2458 Size: 522 96 5203 Fragments arranged by size: 5203 522 96 BstYI r'GATC_y Cuts at: 1972 3003 3151 3518 3903 3920 5372 5458 5470 Size: 1031 148 367 385 17 1452 86 12 Cuts at: 5470 5816 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1031 385 367 346 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2021 2233 2330 2934 513 Size: 552 939 17 212 97 604 3400 Fragments arranged by size: 3400 939 604 552 212 97 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2132 3099 3337 4131 4151 2132 Size: 967 238 794 20 3802 Fragments arranged by size: 3802 967 794 238 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2642 3268 4157 Size: 54 1425 50 275 18 812 626 889 Cuts at: 4157 5786 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 812 626 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3751 1353 Size: 2398 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2607 2690 2902 3884 4481 5268 5536 Size: 393 975 83 212 982 597 787 268 Cuts at: 5536 1239 Size: 1524 Fragments arranged by size: 1524 982 975 787 597 393 268 212 83 EcoO109I rG'GnC_Cy Cuts at: 2473 3038 2473 Size: 565 5256 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2116 4968 1678 Size: 438 2852 2531 Fragments arranged by size: 2852 2531 438 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2065 2097 2696 2728 2736 Size: 32 135 32 493 32 599 32 8 Cuts at: 2736 2768 4462 4494 4536 4568 1373 Size: 32 1694 32 42 32 2626 Fragments arranged by size: 2626 1694 599 493 135 42 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2382 2808 4935 5119 1305 Size: 1077 426 2127 184 2007 Fragments arranged by size: 2127 2007 1077 426 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2137 2606 3779 4138 4297 518 Size: 648 971 469 1173 359 159 2042 Fragments arranged by size: 2042 1173 971 648 469 359 159 MspA1I CmG'CkG Cuts at: 1606 2224 2332 2349 2388 2493 3030 3092 3237 Size: 618 108 17 39 105 537 62 145 Cuts at: 3237 3914 5062 5307 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 618 537 245 145 108 105 62 39 17 NaeI GCC'GGC Cuts at: 2666 2666 Size: 5821 NcoI C'CATG_G Cuts at: 513 1065 2004 2233 513 Size: 552 939 229 4101 Fragments arranged by size: 4101 939 552 229 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2664 2664 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2318 2512 2774 3179 4724 1132 Size: 97 1089 194 262 405 1545 2229 Fragments arranged by size: 2229 1545 1089 405 262 194 97 PasI CC'CwG_GG Cuts at: 1348 2563 3014 1348 Size: 1215 451 4155 Fragments arranged by size: 4155 1215 451 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 1108 Size: 1131 4690 PfoI T'CCnGG_A Cuts at: 2754 2754 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2473 3038 2473 Size: 565 5256 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 2387 3173 830 Size: 1557 786 3478 Fragments arranged by size: 3478 1557 786 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2224 2349 2388 2224 Size: 125 39 5657 Fragments arranged by size: 5657 125 39 SacII CC_GC'GG Cuts at: 2333 2333 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SanDI GG'GwC_CC Cuts at: 3038 3038 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2383 3169 4304 4985 5176 826 Size: 219 1338 786 1135 681 191 1471 Fragments arranged by size: 1471 1338 1135 786 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2256 2256 Size: 5821 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 2723 3925 4826 5088 820 Size: 197 926 540 240 1202 901 262 1553 Fragments arranged by size: 1553 1202 926 901 540 262 240 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2004 2233 2370 2850 3260 513 Size: 552 939 229 137 480 410 3074 Fragments arranged by size: 3074 939 552 480 410 229 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 2829 4067 Size: 274 80 33 51 1443 555 297 1238 Cuts at: 4067 5779 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1238 555 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2267 2299 2398 2430 2504 2536 2663 2695 2860 Size: 32 99 32 74 32 127 32 165 Cuts at: 2860 2892 5781 5813 2267 Size: 32 2889 32 2275 Fragments arranged by size: 2889 2275 165 127 99 74 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2079 1316 Size: 763 5058 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2977 2977 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfoI SrfI SwaI