################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MflI MluI MroI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SpeI SphI Sse8387I SwaI Tth111I Van91I XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 606 GGTACC G^GTACC AccII 497 CGCG CG^CG AcyI 914 GACGCC G[GA]^CG[CT]C AlwI 1198 GATCC GGATC(4/5) ApaI 50 GGGCCC GGGCC^C Asp718I 606 GGTACC G^GTACC AvaI 36 CCCGGG C^[CT]CG[GA]G BanI 606 GGTACC G^G[CT][GA]CC BsaAI 619 TACGTA [CT]AC^GT[GA] BsaHI 914 GACGCC G[GA]^CG[CT]C BsmAI 1131 GTCTC GTCTC(1/5) BsoBI 36 CCCGGG C^[CT]CG[GA]G Bsp1407I 840 TGTACA T^GTACA BsrGI 840 TGTACA T^GTACA BstUI 497 CGCG CG^CG Cac8I 864 GCCTGC GC[ACGT]^[ACGT]GC DpnII 1198 GATC ^GATC DsaI 1222 CCGTGG C^C[GA][CT]GG EcoNI 535 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 229 GATATC GAT^ATC EcoT14I 200 CCTTGG C^C[AT][AT]GG EcoT22I 1411 ATGCAT ATGCA^T Hin1I 914 GACGCC G[GA]^CG[CT]C HphI 462 GGTGA GGTGA(8/7) KpnI 606 GGTACC GGTAC^C MboI 1198 GATC ^GATC MseI 1045 TTAA T^TAA MslI 477 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 497 CGCG CG^CG NdeII 1198 GATC ^GATC NsiI 1411 ATGCAT ATGCA^T Sau3AI 1198 GATC ^GATC SmaI 36 CCCGGG CCC^GGG SnaBI 619 TACGTA TAC^GTA SspBI 840 TGTACA T^GTACA SspI 1075 AATATT AAT^ATT StuI 862 AGGCCT AGG^CCT StyI 200 CCTTGG C^C[AT][AT]GG Tru9I 1045 TTAA T^TAA Tsp45I 1372 GTGAC ^GT[GC]AC XbaI 435 TCTAGA T^CTAGA XmaCI 36 CCCGGG C^CCGGG XmaI 36 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AvaII 967 GGACC G^G[AT]CC 1215 GGACC BalI 203 TGGCCA TGG^CCA 1058 TGGCCA BsmFI 550 GGGAC GGGAC(10/14) 530 GTCCC BstAPI 12 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 175 GCAAGTGTTGC HapII 37 CCGG C^CGG 48 CCGG HgaI 914 GACGC GACGC(5/10) 594 GCGTC HpaII 37 CCGG C^CGG 48 CCGG MluNI 203 TGGCCA TGG^CCA 1058 TGGCCA MscI 203 TGGCCA TGG^CCA 1058 TGGCCA MspI 37 CCGG C^CGG 48 CCGG PpuMI 966 AGGACCT [GA]G^G[AT]CC[CT] 1214 AGGACCC PstI 10 CTGCAG CTGCA^G 217 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AlwNI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboII MnlI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp509I TspRI TthHB8I