(Circular) (Six-base) MAPSORT of: pFN21AE6127.seq Check: 9758 from: 1 to: 5119 pFN21AE6127 5119 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2929 278 Size: 53 83 186 936 1393 2468 Fragments arranged by size: 2468 1393 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2406 2462 1937 Size: 469 56 4594 Fragments arranged by size: 4594 469 56 Acc65I G'GTAC_C Cuts at: 2440 2440 Size: 5119 AclI AA'CG_TT Cuts at: 1563 3246 3619 1563 Size: 1683 373 3063 Fragments arranged by size: 3063 1683 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3182 4566 4834 1632 Size: 695 855 1384 268 1917 Fragments arranged by size: 1917 1384 855 695 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4922 AflIII A'CryG_T Cuts at: 1203 4018 4995 1203 Size: 2815 977 1327 Fragments arranged by size: 2815 1327 977 AgeI A'CCGG_T Cuts at: 3950 3950 Size: 5119 AhdI GACnn_n'nnGTC Cuts at: 3848 3848 Size: 5119 AleI CACnn'nnGTG Cuts at: 1166 2177 1166 Size: 1011 4108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5119 AlwNI CAG_nnn'CTG Cuts at: 2862 4434 2862 Size: 1572 3547 ApaI G_GGCC'C Cuts at: 2321 2321 Size: 5119 ApaLI G'TGCA_C Cuts at: 2395 3176 4332 2395 Size: 781 1156 3182 Fragments arranged by size: 3182 1156 781 ApoI r'AATT_y Cuts at: 1493 2428 2654 2806 4915 1493 Size: 935 226 152 2109 1697 Fragments arranged by size: 2109 1697 935 226 152 AseI AT'TA_AT Cuts at: 160 1032 3673 160 Size: 872 2641 1606 Fragments arranged by size: 2641 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5119 AvaI C'yCGr_G Cuts at: 1870 1943 2444 1870 Size: 73 501 4545 Fragments arranged by size: 4545 501 73 BamHI G'GATC_C Cuts at: 2449 2816 2449 Size: 367 4752 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2440 3895 618 Size: 325 217 627 653 1455 1842 Fragments arranged by size: 1842 1455 653 627 325 217 BanII G_rGCy'C Cuts at: 1391 2321 2438 1391 Size: 930 117 4072 Fragments arranged by size: 4072 930 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4148 BbvCI CC'TCA_GC Cuts at: 2090 2090 Size: 5119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3310 3344 1903 Size: 34 50 34 1289 34 3678 Fragments arranged by size: 3678 1289 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3011 4221 3011 Size: 1210 3909 BclI T'GATC_A Cuts at: 1548 1548 Size: 5119 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3730 136 Size: 107 122 71 631 605 2058 1525 Fragments arranged by size: 2058 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5114 5114 Size: 5119 BlpI GC'TnA_GC Cuts at: 2536 2536 Size: 5119 Bme1580I G_kGCm'C Cuts at: 1790 2321 2399 3180 4336 1790 Size: 531 78 781 1156 2573 Fragments arranged by size: 2573 1156 781 531 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5119 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2850 3808 442 Size: 416 53 83 399 24 1433 958 1753 Fragments arranged by size: 1753 1433 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5119 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3779 1239 Size: 2540 2579 Bpu10I CC'TnA_GC Cuts at: 2090 2090 Size: 5119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3244 4109 4407 3244 Size: 865 298 3956 Fragments arranged by size: 3956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3782 882 Size: 617 2283 2219 Fragments arranged by size: 2283 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2910 493 Size: 711 1706 2702 Fragments arranged by size: 2702 1706 711 BsaBI GATnn'nnATC Cuts at: 5113 5113 Size: 5119 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2926 3308 3943 275 Size: 53 83 186 936 1393 382 635 1451 Fragments arranged by size: 1451 1393 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3552 3950 4224 4371 4782 3552 Size: 398 274 147 411 3889 Fragments arranged by size: 3889 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2233 2263 1178 Size: 30 165 30 830 30 4034 Fragments arranged by size: 4034 830 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2237 1186 Size: 1042 9 4068 Fragments arranged by size: 4068 1042 9 BseYI C'CCAG_C Cuts at: 2071 4322 4818 2071 Size: 2251 496 2372 Fragments arranged by size: 2372 2251 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2042 2569 3330 4358 1540 Size: 275 186 41 527 761 1028 2301 Fragments arranged by size: 2301 1028 761 527 275 186 41 BsiHKAI G_wGCw'C Cuts at: 2399 3180 3265 4336 2399 Size: 781 85 1071 3182 Fragments arranged by size: 3182 1071 781 85 BsmI GAATG_Cn' Cuts at: 2630 2723 2630 Size: 93 5026 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2321 2399 2438 3180 3265 4336 1391 Size: 399 531 78 39 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 85 78 39 BspEI T'CCGG_A Cuts at: 4782 4782 Size: 5119 BspHI T'CATG_A Cuts at: 3008 4738 3008 Size: 1730 3389 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2474 844 Size: 1630 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3006 1394 Size: 429 1183 3507 Fragments arranged by size: 3507 1183 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3614 3788 65 Size: 1183 2366 174 1396 Fragments arranged by size: 2366 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3763 3950 1700 Size: 110 1953 187 2869 Fragments arranged by size: 2869 1953 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5119 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5119 BssSI C'ACGA_G Cuts at: 2329 3179 4191 2329 Size: 850 1012 3257 Fragments arranged by size: 3257 1012 850 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5119 BstBI TT'CG_AA Cuts at: 3923 3923 Size: 5119 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5119 BstYI r'GATC_y Cuts at: 1972 2449 2816 3201 3218 4670 4756 4768 5114 Size: 477 367 385 17 1452 86 12 346 Cuts at: 5114 1972 Size: 1977 Fragments arranged by size: 1977 1452 477 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4567 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4380 Fragments arranged by size: 4380 683 56 BtsI GCAGTG_nn' Cuts at: 2133 2635 3429 3449 2133 Size: 502 794 20 3803 Fragments arranged by size: 3803 794 502 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5119 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5084 DraI TTT'AAA Cuts at: 2423 2423 Size: 5119 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4126 809 Size: 507 2810 1802 Fragments arranged by size: 2810 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2566 3455 5084 Size: 54 1425 50 275 18 736 889 1629 Cuts at: 5084 8 Size: 43 Fragments arranged by size: 1629 1425 889 736 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2566 1537 Size: 275 754 4090 Fragments arranged by size: 4090 754 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3049 1353 Size: 1696 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2396 3706 4080 4226 387 Size: 1064 138 807 1310 374 146 1280 Fragments arranged by size: 1310 1280 1064 807 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 3182 3779 4566 4834 1239 Size: 393 695 855 597 787 268 1524 Fragments arranged by size: 1524 855 787 695 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2244 2317 2318 2244 Size: 73 1 5045 Fragments arranged by size: 5045 73 1 EcoRI G'AATT_C Cuts at: 2428 2806 2428 Size: 378 4741 FspI TGC'GCA Cuts at: 2183 3625 2183 Size: 1442 3677 FspAI rTGC'GCAy Cuts at: 2183 2183 Size: 5119 HaeII r_GCGC'y Cuts at: 1678 4266 1678 Size: 2588 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3760 3792 3834 3866 1373 Size: 32 135 32 2188 32 42 32 2626 Fragments arranged by size: 2626 2188 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2463 2709 669 Size: 988 281 525 246 3079 Fragments arranged by size: 3079 988 525 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5119 HpaI GTT'AAC Cuts at: 2709 2709 Size: 5119 KpnI G_GTAC'C Cuts at: 2444 2444 Size: 5119 MfeI C'AATT_G Cuts at: 2718 2718 Size: 5119 MluI A'CGCG_T Cuts at: 4995 4995 Size: 5119 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4233 4417 1305 Size: 2928 184 2007 Fragments arranged by size: 2928 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3297 Fragments arranged by size: 3297 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 3077 3436 3595 518 Size: 648 1011 900 359 159 2042 Fragments arranged by size: 2042 1011 900 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2535 3212 4360 4605 1606 Size: 929 677 1148 245 2120 Fragments arranged by size: 2120 1148 929 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4567 NdeI CA'TA_TG Cuts at: 387 387 Size: 5119 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5119 NotI GC'GGCC_GC Cuts at: 2566 2566 Size: 5119 NspI r_CATG'y Cuts at: 1132 1229 2477 4022 1132 Size: 97 1248 1545 2229 Fragments arranged by size: 2229 1545 1248 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5119 PciI A'CATG_T Cuts at: 4018 4018 Size: 5119 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5119 PmeI GTTT'AAAC Cuts at: 2423 2423 Size: 5119 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3188 3220 4701 4733 3188 Size: 32 1481 32 3574 Fragments arranged by size: 3574 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5119 PsiI TTA'TAA Cuts at: 2689 2689 Size: 5119 PspOMI G'GGCC_C Cuts at: 2317 2317 Size: 5119 PstI C_TGCA'G Cuts at: 830 2113 2471 830 Size: 1283 358 3478 Fragments arranged by size: 3478 1283 358 PvuI CG_AT'CG Cuts at: 2001 2042 2001 Size: 41 5078 SalI G'TCGA_C Cuts at: 1936 2461 1936 Size: 525 4594 SbfI CC_TGCA'GG Cuts at: 2471 2471 Size: 5119 ScaI AGT'ACT Cuts at: 3367 5079 3367 Size: 1712 3407 SfcI C'TryA_G Cuts at: 826 1045 2109 2467 3602 4283 4474 826 Size: 219 1064 358 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 681 358 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5119 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5119 SmaI CCC'GGG Cuts at: 1872 2446 1872 Size: 574 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3223 4124 4386 820 Size: 197 926 1280 901 262 1553 Fragments arranged by size: 1553 1280 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2910 493 Size: 2417 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5119 SphI G_CATG'C Cuts at: 1132 2477 1132 Size: 1345 3774 SspI AAT'ATT Cuts at: 5 52 3043 5 Size: 47 2991 2081 Fragments arranged by size: 2991 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5119 StyI C'CwwG_G Cuts at: 513 1065 2558 513 Size: 552 1493 3074 Fragments arranged by size: 3074 1493 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3146 3163 3316 3501 3946 325 Size: 1255 1566 17 153 185 445 1498 Fragments arranged by size: 1566 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3365 5077 96 Size: 274 80 33 51 1443 1388 1712 138 Fragments arranged by size: 1712 1443 1388 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5079 5111 5079 Size: 32 5087 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5119 XbaI T'CTAG_A Cuts at: 2455 2455 Size: 5119 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2125 Size: 5119 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5119 XmaI C'CCGG_G Cuts at: 1870 2444 1870 Size: 574 4545 XmnI GAAnn'nnTTC Cuts at: 3248 3248 Size: 5119 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2927 276 Size: 53 83 186 936 1393 2468 Fragments arranged by size: 2468 1393 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI