(Circular) (Six-base) MAPSORT of: pFN21AE1433.seq Check: 423 from: 1 to: 6202 pFN21AE1433 6202 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3545 1937 Size: 1608 4594 Acc65I G'GTAC_C Cuts at: 3523 3523 Size: 6202 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 4265 5649 5917 1632 Size: 398 2235 1384 268 1917 Fragments arranged by size: 2235 1917 1384 398 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 5101 6078 1203 Size: 3898 977 1327 Fragments arranged by size: 3898 1327 977 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 4931 4931 Size: 6202 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6202 AlwNI CAG_nnn'CTG Cuts at: 3945 5517 3945 Size: 1572 4630 ApaI G_GGCC'C Cuts at: 2959 2959 Size: 6202 ApaLI G'TGCA_C Cuts at: 4259 5415 4259 Size: 1156 5046 ApoI r'AATT_y Cuts at: 1493 3511 3737 3889 5998 1493 Size: 2018 226 152 2109 1697 Fragments arranged by size: 2109 2018 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2811 3336 3527 1870 Size: 73 868 525 191 4545 Fragments arranged by size: 4545 868 525 191 73 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2735 3131 3523 4978 618 Size: 325 217 627 948 396 392 1455 1842 Fragments arranged by size: 1842 1455 948 627 396 392 325 217 BanII G_rGCy'C Cuts at: 1391 2822 2959 3246 3362 3521 1391 Size: 1431 137 287 116 159 4072 Fragments arranged by size: 4072 1431 287 159 137 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2498 928 Size: 971 599 4632 Fragments arranged by size: 4632 971 599 BbvCI CC'TCA_GC Cuts at: 2748 2748 Size: 6202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4393 4427 1903 Size: 34 2456 34 3678 Fragments arranged by size: 3678 2456 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2537 4094 5304 2537 Size: 1557 1210 3435 Fragments arranged by size: 3435 1557 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3311 3377 4813 Size: 107 122 71 631 605 1639 66 1436 Cuts at: 4813 136 Size: 1525 Fragments arranged by size: 1639 1525 1436 631 605 122 107 71 66 BglII A'GATC_T Cuts at: 6197 6197 Size: 6202 BlpI GC'TnA_GC Cuts at: 3619 3619 Size: 6202 Bme1580I G_kGCm'C Cuts at: 1790 2738 2959 3134 4263 5419 1790 Size: 948 221 175 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 948 221 175 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3933 4891 442 Size: 416 53 83 399 24 2516 958 1753 Fragments arranged by size: 2516 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3265 3265 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2467 3045 4862 1239 Size: 1228 578 1817 2579 Fragments arranged by size: 2579 1817 1228 578 Bpu10I CC'TnA_GC Cuts at: 2288 2748 2825 2288 Size: 460 77 5665 Fragments arranged by size: 5665 460 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4327 5192 5490 4327 Size: 865 298 5039 Fragments arranged by size: 5039 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3231 3392 4865 882 Size: 617 1732 161 1473 2219 Fragments arranged by size: 2219 1732 1473 617 161 BsaAI yAC'GTr Cuts at: 493 1204 3993 493 Size: 711 2789 2702 Fragments arranged by size: 2789 2702 711 BsaBI GATnn'nnATC Cuts at: 6196 6196 Size: 6202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2321 4009 4391 5026 Size: 53 83 186 936 788 1688 382 635 Cuts at: 5026 275 Size: 1451 Fragments arranged by size: 1688 1451 936 788 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4635 5033 5307 5454 5865 4635 Size: 398 274 147 411 4972 Fragments arranged by size: 4972 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5977 Fragments arranged by size: 5977 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6202 BseYI C'CCAG_C Cuts at: 2952 3036 3060 5405 5901 2952 Size: 84 24 2345 496 3253 Fragments arranged by size: 3253 2345 496 84 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 2774 2789 3486 1163 Size: 573 282 756 15 697 3879 Fragments arranged by size: 3879 756 697 573 282 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3652 4413 5441 1540 Size: 275 186 1651 761 1028 2301 Fragments arranged by size: 2301 1651 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3022 3170 3215 3246 4263 4348 5419 3022 Size: 148 45 31 1017 85 1071 3805 Fragments arranged by size: 3805 1071 1017 148 85 45 31 BsmI GAATG_Cn' Cuts at: 3713 3806 3713 Size: 93 6109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2738 2822 2959 3022 3134 3170 3215 Size: 399 948 84 137 63 112 36 45 Cuts at: 3215 3246 3362 3521 4263 4348 5419 1391 Size: 31 116 159 742 85 1071 2174 Fragments arranged by size: 2174 1071 948 742 399 159 137 116 112 85 84 63 45 36 31 BspEI T'CCGG_A Cuts at: 5865 5865 Size: 6202 BspHI T'CATG_A Cuts at: 4091 5821 4091 Size: 1730 4472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2040 2504 3557 844 Size: 1196 464 1053 3489 Fragments arranged by size: 3489 1196 1053 464 BsrBI CCG'CTC Cuts at: 1394 1823 3451 4089 1394 Size: 429 1628 638 3507 Fragments arranged by size: 3507 1628 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4697 4871 65 Size: 1183 3449 174 1396 Fragments arranged by size: 3449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2159 4846 5033 1700 Size: 110 349 2687 187 2869 Fragments arranged by size: 2869 2687 349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 2081 1925 Size: 156 6046 BssSI C'ACGA_G Cuts at: 4262 5274 4262 Size: 1012 5190 BstAPI GCAn_nnn'nTGC Cuts at: 794 2041 2078 2662 794 Size: 1247 37 584 4334 Fragments arranged by size: 4334 1247 584 37 BstBI TT'CG_AA Cuts at: 2788 5006 2788 Size: 2218 3984 BstEII G'GTnAC_C Cuts at: 1184 2231 1184 Size: 1047 5155 BstXI CCAn_nnnn'nTGG Cuts at: 3115 3115 Size: 6202 BstYI r'GATC_y Cuts at: 1972 2391 3532 3899 4284 4301 5753 5839 5851 Size: 419 1141 367 385 17 1452 86 12 Cuts at: 5851 6197 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1141 419 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3404 3404 Size: 6202 BtgI C'CryG_G Cuts at: 513 1065 2004 2601 3189 3256 3300 513 Size: 552 939 597 588 67 44 3415 Fragments arranged by size: 3415 939 597 588 552 67 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2234 487 Size: 683 56 1008 4455 Fragments arranged by size: 4455 1008 683 56 BtsI GCAGTG_nn' Cuts at: 2260 2749 3718 4512 4532 2260 Size: 489 969 794 20 3930 Fragments arranged by size: 3930 969 794 489 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DraIII CAC_nnn'GTG Cuts at: 2908 2921 2908 Size: 13 6189 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2274 2598 2866 Size: 54 1425 50 275 18 444 324 268 Cuts at: 2866 2910 2923 2976 3303 3649 4538 6167 8 Size: 44 13 53 327 346 889 1629 43 Fragments arranged by size: 1629 1425 889 444 346 327 324 275 268 54 53 50 44 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3649 1537 Size: 275 1837 4090 Fragments arranged by size: 4090 1837 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4132 1353 Size: 2779 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4789 5163 5309 387 Size: 1064 138 3200 374 146 1280 Fragments arranged by size: 3200 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3244 3244 Size: 6202 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2467 3045 4265 4862 5649 5917 Size: 393 398 437 578 1220 597 787 268 Cuts at: 5917 1239 Size: 1524 Fragments arranged by size: 1524 1220 787 597 578 437 398 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2291 2291 Size: 6202 EcoO109I rG'GnC_Cy Cuts at: 2956 3174 3340 2956 Size: 218 166 5818 Fragments arranged by size: 5818 218 166 EcoRI G'AATT_C Cuts at: 3511 3889 3511 Size: 378 5824 FspI TGC'GCA Cuts at: 4708 4708 Size: 6202 HaeII r_GCGC'y Cuts at: 1678 2160 3418 5349 1678 Size: 482 1258 1931 2531 Fragments arranged by size: 2531 1931 1258 482 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 4843 4875 4917 Size: 32 135 32 449 32 2790 32 42 Cuts at: 4917 4949 1373 Size: 32 2626 Fragments arranged by size: 2790 2626 449 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3546 3792 669 Size: 988 281 1608 246 3079 Fragments arranged by size: 3079 1608 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6202 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KpnI G_GTAC'C Cuts at: 3527 3527 Size: 6202 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3408 5316 5500 1305 Size: 2103 1908 184 2007 Fragments arranged by size: 2103 2007 1908 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 2868 2912 2925 2978 Size: 54 1425 343 444 592 44 13 53 Cuts at: 2978 3305 10 Size: 327 2907 Fragments arranged by size: 2907 1425 592 444 343 327 54 53 44 13 MslI CAynn'nnrTG Cuts at: 518 1166 2253 2880 2883 4160 4519 4678 518 Size: 648 1087 627 3 1277 359 159 2042 Fragments arranged by size: 2042 1277 1087 648 627 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2050 3618 4295 5443 5688 1606 Size: 444 1568 677 1148 245 2120 Fragments arranged by size: 2120 1568 1148 677 444 245 NaeI GCC'GGC Cuts at: 2161 2161 Size: 6202 NcoI C'CATG_G Cuts at: 513 1065 2004 3256 3300 513 Size: 552 939 1252 44 3415 Fragments arranged by size: 3415 1252 939 552 44 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NgoMIV G'CCGG_C Cuts at: 2159 2159 Size: 6202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 3560 5105 1132 Size: 97 2331 1545 2229 Fragments arranged by size: 2331 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6202 PciI A'CATG_T Cuts at: 5101 5101 Size: 6202 PflMI CCAn_nnn'nTGG Cuts at: 1108 3222 1108 Size: 2114 4088 PfoI T'CCnGG_A Cuts at: 2529 3022 2529 Size: 493 5709 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 5784 5816 4271 Size: 32 1481 32 4657 Fragments arranged by size: 4657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3340 3340 Size: 6202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6202 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PspOMI G'GGCC_C Cuts at: 2955 2955 Size: 6202 PstI C_TGCA'G Cuts at: 830 2658 3051 3554 830 Size: 1828 393 503 3478 Fragments arranged by size: 3478 1828 503 393 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6202 PvuII CAG'CTG Cuts at: 2050 2050 Size: 6202 SacI G_AGCT'C Cuts at: 3246 3246 Size: 6202 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 2454 4450 6162 2454 Size: 1996 1712 2494 Fragments arranged by size: 2494 1996 1712 SfcI C'TryA_G Cuts at: 826 1045 2458 2654 3047 3550 4685 5366 5557 Size: 219 1413 196 393 503 1135 681 191 Cuts at: 5557 826 Size: 1471 Fragments arranged by size: 1471 1413 1135 681 503 393 219 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 3338 3529 1872 Size: 1466 191 4545 Fragments arranged by size: 4545 1466 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 4306 5207 5469 820 Size: 197 926 2363 901 262 1553 Fragments arranged by size: 2363 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3560 1132 Size: 2428 3774 SspI AAT'ATT Cuts at: 5 52 4126 5 Size: 47 4074 2081 Fragments arranged by size: 4074 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6202 StyI C'CwwG_G Cuts at: 513 1065 2004 2100 2199 3256 3300 3306 3490 Size: 552 939 96 99 1057 44 6 184 Cuts at: 3490 3641 513 Size: 151 3074 Fragments arranged by size: 3074 1057 939 552 184 151 99 96 44 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2074 2143 3074 4229 4246 4399 4584 Size: 1255 494 69 931 1155 17 153 185 Cuts at: 4584 5029 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1155 931 494 445 185 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2452 2593 4448 Size: 274 80 33 51 1443 475 141 1855 Cuts at: 4448 6160 96 Size: 1712 138 Fragments arranged by size: 1855 1712 1443 475 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6162 6194 6162 Size: 32 6170 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6202 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2939 3307 2939 Size: 368 5834 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 3336 3527 1870 Size: 1466 191 4545 Fragments arranged by size: 4545 1466 191 XmnI GAAnn'nnTTC Cuts at: 4331 4331 Size: 6202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI