(Circular) (Six-base) MAPSORT of: pFN21AE0745.seq Check: 3867 from: 1 to: 5665 pFN21AE0745 5665 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2570 3008 1937 Size: 633 438 4594 Fragments arranged by size: 4594 633 438 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 2964 3792 4165 1563 Size: 1401 828 373 3063 Fragments arranged by size: 3063 1401 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 3728 5112 5380 1632 Size: 610 1486 1384 268 1917 Fragments arranged by size: 1917 1486 1384 610 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 2753 4564 5541 1203 Size: 1550 1811 977 1327 Fragments arranged by size: 1811 1550 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AlwNI CAG_nnn'CTG Cuts at: 3408 4980 3408 Size: 1572 4093 ApaI G_GGCC'C Cuts at: 2535 2535 Size: 5665 ApaLI G'TGCA_C Cuts at: 3722 4878 3722 Size: 1156 4509 ApoI r'AATT_y Cuts at: 1493 2974 3200 3352 5461 1493 Size: 1481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2990 1870 Size: 73 1047 4545 Fragments arranged by size: 4545 1047 73 BamHI G'GATC_C Cuts at: 2103 2995 3362 2103 Size: 892 367 4406 Fragments arranged by size: 4406 892 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4441 618 Size: 325 217 627 1199 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 BanII G_rGCy'C Cuts at: 1391 2094 2158 2535 2778 2984 1391 Size: 703 64 377 243 206 4072 Fragments arranged by size: 4072 703 377 243 206 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2864 2898 3856 3890 1903 Size: 34 50 34 843 34 958 34 3678 Fragments arranged by size: 3678 958 843 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2515 3557 4767 2515 Size: 1042 1210 3413 Fragments arranged by size: 3413 1210 1042 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 136 Size: 107 122 71 631 605 2604 1525 Fragments arranged by size: 2604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2535 3726 4882 1790 Size: 745 1191 1156 2573 Fragments arranged by size: 2573 1191 1156 745 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2028 3396 4354 Size: 416 53 83 399 24 611 1368 958 Cuts at: 4354 442 Size: 1753 Fragments arranged by size: 1753 1368 958 611 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 4325 1239 Size: 795 2291 2579 Fragments arranged by size: 2579 2291 795 Bpu10I CC'TnA_GC Cuts at: 2817 2817 Size: 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 2435 3790 4655 4953 2081 Size: 354 1355 865 298 2793 Fragments arranged by size: 2793 1355 865 354 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2261 2753 4328 882 Size: 617 762 492 1575 2219 Fragments arranged by size: 2219 1575 762 617 492 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5440 Fragments arranged by size: 5440 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2072 2084 2441 2444 1186 Size: 883 3 12 357 3 4407 Fragments arranged by size: 4407 883 357 12 3 3 BseYI C'CCAG_C Cuts at: 2527 2560 4868 5364 2527 Size: 33 2308 496 2828 Fragments arranged by size: 2828 2308 496 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2633 1163 Size: 573 897 4195 Fragments arranged by size: 4195 897 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 2158 2778 3726 3811 4882 2094 Size: 64 620 948 85 1071 2877 Fragments arranged by size: 2877 1071 948 620 85 64 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2158 2535 2778 2984 3726 3811 Size: 399 304 64 377 243 206 742 85 Cuts at: 3811 4882 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 377 304 243 206 85 64 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2775 3020 844 Size: 1931 245 3489 Fragments arranged by size: 3489 1931 245 BsrBI CCG'CTC Cuts at: 1394 1823 3552 1394 Size: 429 1729 3507 Fragments arranged by size: 3507 1729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2892 4160 4334 65 Size: 1183 1644 1268 174 1396 Fragments arranged by size: 1644 1396 1268 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2608 4309 4496 1700 Size: 110 798 1701 187 2869 Fragments arranged by size: 2869 1701 798 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5665 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5665 BstBI TT'CG_AA Cuts at: 2027 4469 2027 Size: 2442 3223 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2103 2995 3362 3747 3764 5216 5302 5314 Size: 131 892 367 385 17 1452 86 12 Cuts at: 5314 5660 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 892 385 367 346 131 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2677 513 Size: 552 1612 3501 Fragments arranged by size: 3501 1612 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 2159 3181 3975 3995 2159 Size: 1022 794 20 3829 Fragments arranged by size: 3829 1022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2078 4672 809 Size: 507 762 2594 1802 Fragments arranged by size: 2594 1802 762 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2355 2550 Size: 54 1425 50 275 18 282 243 195 Cuts at: 2550 2833 3112 4001 5630 8 Size: 283 279 889 1629 43 Fragments arranged by size: 1629 1425 889 283 282 279 275 243 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2783 3595 1353 Size: 1430 812 3423 Fragments arranged by size: 3423 1430 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2092 2156 2776 2092 Size: 64 620 4981 Fragments arranged by size: 4981 620 64 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2242 3728 4325 5112 5380 1239 Size: 393 402 208 1486 597 787 268 1524 Fragments arranged by size: 1524 1486 787 597 402 393 268 208 EcoNI CCTnn'n_nnAGG Cuts at: 2816 2816 Size: 5665 EcoO109I rG'GnC_Cy Cuts at: 2410 2434 2531 2410 Size: 24 97 5544 Fragments arranged by size: 5544 97 24 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 4812 1678 Size: 3134 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 4306 4338 4380 Size: 32 135 32 794 32 1908 32 42 Cuts at: 4380 4412 1373 Size: 32 2626 Fragments arranged by size: 2626 1908 794 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 2753 5541 2753 Size: 2788 2877 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4779 4963 1305 Size: 3474 184 2007 Fragments arranged by size: 3474 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2114 2357 2835 10 Size: 54 1425 343 282 243 478 2840 Fragments arranged by size: 2840 1425 478 343 282 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 3623 3982 4141 518 Size: 648 2457 359 159 2042 Fragments arranged by size: 2457 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2508 2560 2679 3081 3758 4906 5151 1606 Size: 902 52 119 402 677 1148 245 2120 Fragments arranged by size: 2120 1148 902 677 402 245 119 52 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5113 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 3023 4568 1132 Size: 97 1794 1545 2229 Fragments arranged by size: 2229 1794 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2434 2410 Size: 24 5641 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PspOMI G'GGCC_C Cuts at: 2531 2531 Size: 5665 PstI C_TGCA'G Cuts at: 830 2772 3017 830 Size: 1942 245 3478 Fragments arranged by size: 3478 1942 245 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 PvuII CAG'CTG Cuts at: 2560 2560 Size: 5665 SacI G_AGCT'C Cuts at: 2094 2158 2778 2094 Size: 64 620 4981 Fragments arranged by size: 4981 620 64 SacII CC_GC'GG Cuts at: 2680 2680 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SapI GCTCTTCn'nnn_ Cuts at: 2783 2783 Size: 5665 SbfI CC_TGCA'GG Cuts at: 2772 3017 2772 Size: 245 5420 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SexAI A'CCwGG_T Cuts at: 2046 2046 Size: 5665 SfcI C'TryA_G Cuts at: 826 1045 2768 3013 4148 4829 5020 826 Size: 219 1723 245 1135 681 191 1471 Fragments arranged by size: 1723 1471 1135 681 245 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2992 1872 Size: 1120 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 2450 3769 4670 4932 820 Size: 197 926 153 354 1319 901 262 1553 Fragments arranged by size: 1553 1319 926 901 354 262 197 153 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5665 StyI C'CwwG_G Cuts at: 513 1065 2172 2841 3104 513 Size: 552 1107 669 263 3074 Fragments arranged by size: 3074 1107 669 552 263 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2777 3692 3709 3862 4047 4492 325 Size: 1255 1197 915 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1197 915 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 3911 5623 Size: 274 80 33 51 1443 234 1700 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1712 1700 1443 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2337 2369 5625 5657 2337 Size: 32 3256 32 2345 Fragments arranged by size: 3256 2345 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2378 1316 Size: 1062 4603 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2990 1870 Size: 1120 4545 XmnI GAAnn'nnTTC Cuts at: 2315 3794 2315 Size: 1479 4186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI XcmI