################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BbeI BbrPI BbvCI BclI BfrI BglII BlnI BlpI Bpu1102I BsaHI BseAI BseRI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MboI MfeI MflI MluI MroI MseI MunI NarI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflIII 345 ACGTGT A^C[GA][CT]GT ApaI 1100 GGGCCC GGGCC^C AviII 425 TGCGCA TGC^GCA BbsI 874 GTCTTC GAAGAC(2/6) BciVI 255 GTATCC GTATCC(6/5) BglI 1345 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 874 GTCTTC GAAGAC(2/6) BsaAI 1060 TACGTG [CT]AC^GT[GA] BsaBI 445 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1012 GAGACC GGTCTC(1/5) BsaWI 326 ACCGGA [AT]^CCGG[AT] BsiEI 240 CGACCG CG[GA][CT]^CG BsmI 171 GAATGC GAATGC(1/-1) BspHI 639 TCATGA T^CATGA BstAPI 552 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 975 CATCC GGATG(2/0) DraIII 1202 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 742 CTCTTC CTCTTC(1/4) FokI 975 CATCC GGATG(9/13) FseI 97 GGCCGGCC GGCCGG^CC FspI 425 TGCGCA TGC^GCA HaeII 1121 GGCGCT [GA]GCGC^[CT] HgaI 988 GACGC GACGC(5/10) Ksp632I 742 CTCTTC CTCTTC(1/4) MamI 445 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 98 GCCGGC GCC^GGC NgoMIV 98 GCCGGC G^CCGGC PflMI 468 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 373 GAGTC GAGTC(4/5) PpuMI 1363 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 639 TCATGA T^CATGA SexAI 1203 ACCTGGT A^CC[AT]GGT TfiI 268 GATTC G^A[AT]TC Van91I 468 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 20 CCACCGTTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 754 GTCTAC GT^[AC][GT]AC 868 GTCTAC AccII 238 CGCG CG^CG 990 CGCG BcgI 765 CGAGACACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 354 GCACTACATTCG BcnI 596 CCCGG CC^[GC]GG 1098 CCGGG BsgI 184 GTGCAG GTGCAG(16/14) 649 GTGCAG BspMI 1015 ACCTGC ACCTGC(4/8) 1259 ACCTGC BsrDI 701 GCAATG GCAATG(2/0) 1308 GCAATG BsrFI 98 GCCGGC [GA]^CCGG[CT] 455 GCCGGT BstUI 238 CGCG CG^CG 990 CGCG Cfr10I 98 GCCGGC [GA]^CCGG[CT] 455 GCCGGT DrdI 366 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 505 GACCGCATAGTC HinfI 268 GATTC G^A[ACGT]TC 373 GAGTC MvnI 238 CGCG CG^CG 990 CGCG NciI 596 CCCGG CC^[GC]GG 1098 CCGGG PstI 337 CTGCAG CTGCA^G 1261 CTGCAG SacI 64 GAGCTC GAGCT^C 1234 GAGCTC SfaNI 333 GATGC GCATC(5/9) 1161 GCATC SmlI 67 CTCAAG C^T[CT][GA]AG 151 CTCAAG SspI 220 AATATT AAT^ATT 1088 AATATT TaqI 266 TCGA T^CGA 395 TCGA Tsp45I 485 GTCAC ^GT[GC]AC 986 GTGAC TthHB8I 266 TCGA T^CGA 395 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BalI BanI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MaeII MaeIII MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NcoI NlaIII NlaIV PvuII RsaI Sau96I ScrFI SfcI StuI StyI TseI TspRI