(Circular) (Six-base) MAPSORT of: pFN21AE1543.seq Check: 8669 from: 1 to: 6124 pFN21AE1543 6124 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3273 3273 Size: 6124 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 2874 3467 1937 Size: 823 114 593 4594 Fragments arranged by size: 4594 823 593 114 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4187 5571 5839 1632 Size: 2555 1384 268 1917 Fragments arranged by size: 2555 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 2350 5023 6000 1203 Size: 1147 2673 977 1327 Fragments arranged by size: 2673 1327 1147 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 4853 4853 Size: 6124 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AlwNI CAG_nnn'CTG Cuts at: 3867 5439 3867 Size: 1572 4552 ApaI G_GGCC'C Cuts at: 3109 3109 Size: 6124 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 3433 3659 3811 5920 1493 Size: 1940 226 152 2109 1697 Fragments arranged by size: 2109 1940 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2161 2604 3347 3445 4900 Size: 325 217 627 374 443 743 98 1455 Cuts at: 4900 618 Size: 1842 Fragments arranged by size: 1842 1455 743 627 443 374 325 217 98 BanII G_rGCy'C Cuts at: 1391 2073 3109 3243 3443 1391 Size: 682 1036 134 200 4072 Fragments arranged by size: 4072 1036 682 200 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2872 928 Size: 971 973 4180 Fragments arranged by size: 4180 973 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2348 2382 2759 2793 4315 Size: 34 50 34 327 34 377 34 1522 Cuts at: 4315 4349 1903 Size: 34 3678 Fragments arranged by size: 3678 1522 377 327 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2271 4016 5226 2271 Size: 1745 1210 3169 Fragments arranged by size: 3169 1745 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3356 4735 Size: 107 122 71 631 605 337 1347 1379 Cuts at: 4735 136 Size: 1525 Fragments arranged by size: 1525 1379 1347 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6119 6119 Size: 6124 BlpI GC'TnA_GC Cuts at: 3541 3541 Size: 6124 Bme1580I G_kGCm'C Cuts at: 1790 2133 2329 3109 4185 5341 1790 Size: 343 196 780 1076 1156 2573 Fragments arranged by size: 2573 1156 1076 780 343 196 BmgBI CAC'GTC Cuts at: 1318 2128 1318 Size: 810 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2413 2671 2864 Size: 416 53 83 399 24 996 258 193 Cuts at: 2864 3103 3141 3855 4813 442 Size: 239 38 714 958 1753 Fragments arranged by size: 1753 996 958 714 416 399 258 239 193 83 53 38 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2490 2588 3322 3363 4784 1239 Size: 1251 98 734 41 1421 2579 Fragments arranged by size: 2579 1421 1251 734 98 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2057 2141 4249 5114 5412 2057 Size: 84 2108 865 298 2769 Fragments arranged by size: 2769 2108 865 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3011 4787 882 Size: 617 1512 1776 2219 Fragments arranged by size: 2219 1776 1512 617 BsaAI yAC'GTr Cuts at: 493 1204 3067 3915 493 Size: 711 1863 848 2702 Fragments arranged by size: 2702 1863 848 711 BsaBI GATnn'nnATC Cuts at: 2454 6118 2454 Size: 3664 2460 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3931 4313 4948 275 Size: 53 83 186 936 2398 382 635 1451 Fragments arranged by size: 2398 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2331 4557 4955 5229 5376 5787 2331 Size: 2226 398 274 147 411 2668 Fragments arranged by size: 2668 2226 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5899 Fragments arranged by size: 5899 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6124 BseYI C'CCAG_C Cuts at: 3219 3365 5327 5823 3219 Size: 146 1962 496 3520 Fragments arranged by size: 3520 1962 496 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 2675 1163 Size: 573 474 465 4612 Fragments arranged by size: 4612 573 474 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2248 3574 4335 5363 1540 Size: 275 186 247 1326 761 1028 2301 Fragments arranged by size: 2301 1326 1028 761 275 247 186 BsiHKAI G_wGCw'C Cuts at: 2073 2361 2741 3243 4185 4270 5341 2073 Size: 288 380 502 942 85 1071 2856 Fragments arranged by size: 2856 1071 942 502 380 288 85 BsmI GAATG_Cn' Cuts at: 2182 3635 3728 2182 Size: 1453 93 4578 Fragments arranged by size: 4578 1453 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2133 2329 2361 2741 3109 3243 Size: 399 283 60 196 32 380 368 134 Cuts at: 3243 3443 4185 4270 5341 1391 Size: 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 368 283 200 196 134 85 60 32 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 2644 4013 5743 2644 Size: 1369 1730 3025 Fragments arranged by size: 3025 1730 1369 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 3273 3479 844 Size: 2185 244 206 3489 Fragments arranged by size: 3489 2185 244 206 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2713 3320 4619 4793 65 Size: 1183 1465 607 1299 174 1396 Fragments arranged by size: 1465 1396 1299 1183 607 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 2460 4768 4955 1700 Size: 110 293 357 2308 187 2869 Fragments arranged by size: 2869 2308 357 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 2563 794 Size: 1769 4355 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstXI CCAn_nnnn'nTGG Cuts at: 3116 3122 3209 3388 3116 Size: 6 87 179 5852 Fragments arranged by size: 5852 179 87 6 BstYI r'GATC_y Cuts at: 1972 3454 3821 4206 4223 5675 5761 5773 6119 Size: 1482 367 385 17 1452 86 12 346 Cuts at: 6119 1972 Size: 1977 Fragments arranged by size: 1977 1482 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2064 2791 3115 3121 513 Size: 552 999 727 324 6 3516 Fragments arranged by size: 3516 999 727 552 324 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 2573 2598 3640 4434 4454 2573 Size: 25 1042 794 20 4243 Fragments arranged by size: 4243 1042 794 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3302 3337 529 Size: 35 2738 35 3316 Fragments arranged by size: 3316 2738 35 35 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DraIII CAC_nnn'GTG Cuts at: 3212 3212 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2377 2516 5131 809 Size: 507 1061 139 2615 1802 Fragments arranged by size: 2615 1802 1061 507 139 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 2481 2794 Size: 54 1425 50 275 18 271 380 313 Cuts at: 2794 3118 3199 3571 4460 6089 8 Size: 324 81 372 889 1629 43 Fragments arranged by size: 1629 1425 889 380 372 324 313 275 271 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2753 4054 1353 Size: 1400 1301 3423 Fragments arranged by size: 3423 1400 1301 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3176 4711 5085 5231 387 Size: 1064 138 1587 1535 374 146 1280 Fragments arranged by size: 1587 1535 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2071 3241 2071 Size: 1170 4954 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2490 2588 3322 3363 4187 4784 5571 Size: 393 858 98 734 41 824 597 787 Cuts at: 5571 5839 1239 Size: 268 1524 Fragments arranged by size: 1524 858 824 787 734 597 393 268 98 41 EcoO109I rG'GnC_Cy Cuts at: 2021 2827 3369 2021 Size: 806 542 4776 Fragments arranged by size: 4776 806 542 EcoRI G'AATT_C Cuts at: 3433 3811 3433 Size: 378 5746 FseI GG_CCGG'CC Cuts at: 2107 2107 Size: 6124 FspI TGC'GCA Cuts at: 2432 4630 2432 Size: 2198 3926 HaeII r_GCGC'y Cuts at: 1678 3130 5271 1678 Size: 1452 2141 2531 Fragments arranged by size: 2531 2141 1452 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2921 2953 4765 4797 4839 Size: 32 135 32 1349 32 1812 32 42 Cuts at: 4839 4871 1373 Size: 32 2626 Fragments arranged by size: 2626 1812 1349 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3468 3714 669 Size: 988 281 1530 246 3079 Fragments arranged by size: 3079 1530 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5238 5422 1305 Size: 3933 184 2007 Fragments arranged by size: 3933 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2483 3120 3201 10 Size: 54 1425 343 651 637 81 2933 Fragments arranged by size: 2933 1425 651 637 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 2649 3120 4082 4441 4600 518 Size: 648 1132 351 471 962 359 159 2042 Fragments arranged by size: 2042 1132 962 648 471 359 351 159 MspA1I CmG'CkG Cuts at: 1606 2682 2716 2972 3223 3282 3341 3540 4217 Size: 1076 34 256 251 59 59 199 677 Cuts at: 4217 5365 5610 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1076 677 256 251 245 199 59 59 34 NaeI GCC'GGC Cuts at: 2105 2105 Size: 6124 NcoI C'CATG_G Cuts at: 513 1065 2064 2791 3115 3121 513 Size: 552 999 727 324 6 3516 Fragments arranged by size: 3516 999 727 552 324 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NgoMIV G'CCGG_C Cuts at: 2103 2103 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 3482 5027 1132 Size: 97 2253 1545 2229 Fragments arranged by size: 2253 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2962 1348 Size: 1614 4510 PciI A'CATG_T Cuts at: 5023 5023 Size: 6124 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 1108 Size: 1371 4753 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 4193 4225 5706 5738 2528 Size: 32 1633 32 1481 32 2914 Fragments arranged by size: 2914 1633 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3369 3369 Size: 6124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PspOMI G'GGCC_C Cuts at: 3105 3105 Size: 6124 PstI C_TGCA'G Cuts at: 830 2346 3270 3476 830 Size: 1516 924 206 3478 Fragments arranged by size: 3478 1516 924 206 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 PvuII CAG'CTG Cuts at: 2716 2972 3223 3341 2716 Size: 256 251 118 5499 Fragments arranged by size: 5499 256 251 118 SacI G_AGCT'C Cuts at: 2073 3243 2073 Size: 1170 4954 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SanDI GG'GwC_CC Cuts at: 3369 3369 Size: 6124 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SexAI A'CCwGG_T Cuts at: 3208 3208 Size: 6124 SfcI C'TryA_G Cuts at: 826 1045 2342 2896 3266 3472 4607 5288 5479 Size: 219 1297 554 370 206 1135 681 191 Cuts at: 5479 826 Size: 1471 Fragments arranged by size: 1471 1297 1135 681 554 370 219 206 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 2156 4228 5129 5391 820 Size: 197 926 129 84 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 129 84 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3482 1132 Size: 2350 3774 SspI AAT'ATT Cuts at: 5 52 2227 3095 4048 5 Size: 47 2175 868 953 2081 Fragments arranged by size: 2175 2081 953 868 47 StuI AGG'CCT Cuts at: 1517 2195 2256 3359 1517 Size: 678 61 1103 4282 Fragments arranged by size: 4282 1103 678 61 StyI C'CwwG_G Cuts at: 513 1065 2064 2791 3115 3121 3420 3563 513 Size: 552 999 727 324 6 299 143 3074 Fragments arranged by size: 3074 999 727 552 324 299 143 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2104 2416 2861 3104 4151 4168 4321 Size: 1255 524 312 445 243 1047 17 153 Cuts at: 4321 4506 4951 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1047 524 445 445 312 243 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4370 6082 96 Size: 274 80 33 51 1443 2393 1712 138 Fragments arranged by size: 2393 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 6084 6116 2274 Size: 32 3778 32 2282 Fragments arranged by size: 3778 2282 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2032 2032 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI