(Linear) (Six-base) MAPSORT of: KIEE1543.seq Check: 2266 from: 1 to: 1419 KIEE1543.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1268 1419 Size: 1268 151 AccI GT'mk_AC Cuts at: 0 755 869 1419 Size: 755 114 550 Fragments arranged by size: 755 550 114 AflIII A'CryG_T Cuts at: 0 345 1419 Size: 345 1074 ApaI G_GGCC'C Cuts at: 0 1104 1419 Size: 1104 315 BanI G'GyrC_C Cuts at: 0 156 599 1342 1419 Size: 156 443 743 77 Fragments arranged by size: 743 443 156 77 BanII G_rGCy'C Cuts at: 0 68 1104 1238 1419 Size: 68 1036 134 181 Fragments arranged by size: 1036 181 134 68 BbsI GAAGACnn'nnnn_ Cuts at: 0 867 1419 Size: 867 552 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 343 377 754 788 1419 Size: 343 34 377 34 631 Fragments arranged by size: 631 377 343 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 266 1419 Size: 266 1153 BglI GCCn_nnn'nGGC Cuts at: 0 1351 1419 Size: 1351 68 Bme1580I G_kGCm'C Cuts at: 0 128 324 1104 1419 Size: 128 196 780 315 Fragments arranged by size: 780 315 196 128 BmgBI CAC'GTC Cuts at: 0 123 1419 Size: 123 1296 BmrI ACTGGGnnnn_n' Cuts at: 0 408 666 859 1098 1136 1419 Size: 408 258 193 239 38 283 Fragments arranged by size: 408 283 258 239 193 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 485 583 1317 1358 1419 Size: 485 98 734 41 61 Fragments arranged by size: 734 485 98 61 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 136 1419 Size: 52 84 1283 Fragments arranged by size: 1283 84 52 BsaI GGTCTCn'nnnn_ Cuts at: 0 1006 1419 Size: 1006 413 BsaAI yAC'GTr Cuts at: 0 1062 1419 Size: 1062 357 BsaBI GATnn'nnATC Cuts at: 0 449 1419 Size: 449 970 BsaWI w'CCGG_w Cuts at: 0 326 1419 Size: 326 1093 BseYI C'CCAG_C Cuts at: 0 1214 1360 1419 Size: 1214 146 59 Fragments arranged by size: 1214 146 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 670 1419 Size: 205 465 749 Fragments arranged by size: 749 465 205 BsiEI CG_ry'CG Cuts at: 0 243 1419 Size: 243 1176 BsiHKAI G_wGCw'C Cuts at: 0 68 356 736 1238 1419 Size: 68 288 380 502 181 Fragments arranged by size: 502 380 288 181 68 BsmI GAATG_Cn' Cuts at: 0 177 1419 Size: 177 1242 Bsp1286I G_dGCh'C Cuts at: 0 68 128 324 356 736 1104 1238 1419 Size: 68 60 196 32 380 368 134 181 Fragments arranged by size: 380 368 196 181 134 68 60 32 BspHI T'CATG_A Cuts at: 0 639 1419 Size: 639 780 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1024 1268 1419 Size: 1024 244 151 Fragments arranged by size: 1024 244 151 BsrDI GCAATG_nn' Cuts at: 0 708 1315 1419 Size: 708 607 104 Fragments arranged by size: 708 607 104 BsrFI r'CCGG_y Cuts at: 0 98 455 1419 Size: 98 357 964 Fragments arranged by size: 964 357 98 BstAPI GCAn_nnn'nTGC Cuts at: 0 558 1419 Size: 558 861 BstXI CCAn_nnnn'nTGG Cuts at: 0 1111 1117 1204 1383 1419 Size: 1111 6 87 179 36 Fragments arranged by size: 1111 179 87 36 6 BtgI C'CryG_G Cuts at: 0 59 786 1110 1116 1419 Size: 59 727 324 6 303 Fragments arranged by size: 727 324 303 59 6 BtsI GCAGTG_nn' Cuts at: 0 568 593 1419 Size: 568 25 826 Fragments arranged by size: 826 568 25 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1297 1332 1419 Size: 1297 35 87 Fragments arranged by size: 1297 87 35 DraIII CAC_nnn'GTG Cuts at: 0 1207 1419 Size: 1207 212 DrdI GACnn_nn'nnGTC Cuts at: 0 372 511 1419 Size: 372 139 908 Fragments arranged by size: 908 372 139 EaeI y'GGCC_r Cuts at: 0 96 476 789 1113 1194 1419 Size: 96 380 313 324 81 225 Fragments arranged by size: 380 324 313 225 96 81 EarI CTCTTCn'nnn_ Cuts at: 0 748 1419 Size: 748 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1171 1419 Size: 1171 248 EcoICRI GAG'CTC Cuts at: 0 66 1236 1419 Size: 66 1170 183 Fragments arranged by size: 1170 183 66 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 485 583 1317 1358 1419 Size: 485 98 734 41 61 Fragments arranged by size: 734 485 98 61 41 EcoO109I rG'GnC_Cy Cuts at: 0 16 822 1364 1419 Size: 16 806 542 55 Fragments arranged by size: 806 542 55 16 FseI GG_CCGG'CC Cuts at: 0 102 1419 Size: 102 1317 FspI TGC'GCA Cuts at: 0 427 1419 Size: 427 992 HaeII r_GCGC'y Cuts at: 0 1125 1419 Size: 1125 294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 916 948 1419 Size: 916 32 471 Fragments arranged by size: 916 471 32 MscI TGG'CCA Cuts at: 0 478 1115 1196 1419 Size: 478 637 81 223 Fragments arranged by size: 637 478 223 81 MslI CAynn'nnrTG Cuts at: 0 293 644 1115 1419 Size: 293 351 471 304 Fragments arranged by size: 471 351 304 293 MspA1I CmG'CkG Cuts at: 0 677 711 967 1218 1277 1336 1419 Size: 677 34 256 251 59 59 83 Fragments arranged by size: 677 256 251 83 59 59 34 NaeI GCC'GGC Cuts at: 0 100 1419 Size: 100 1319 NcoI C'CATG_G Cuts at: 0 59 786 1110 1116 1419 Size: 59 727 324 6 303 Fragments arranged by size: 727 324 303 59 6 NgoMIV G'CCGG_C Cuts at: 0 98 1419 Size: 98 1321 PasI CC'CwG_GG Cuts at: 0 957 1419 Size: 957 462 PflMI CCAn_nnn'nTGG Cuts at: 0 474 1419 Size: 474 945 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 523 555 1419 Size: 523 32 864 Fragments arranged by size: 864 523 32 PpuMI rG'GwC_Cy Cuts at: 0 1364 1419 Size: 1364 55 PspOMI G'GGCC_C Cuts at: 0 1100 1419 Size: 1100 319 PstI C_TGCA'G Cuts at: 0 341 1265 1419 Size: 341 924 154 Fragments arranged by size: 924 341 154 PvuII CAG'CTG Cuts at: 0 711 967 1218 1336 1419 Size: 711 256 251 118 83 Fragments arranged by size: 711 256 251 118 83 SacI G_AGCT'C Cuts at: 0 68 1238 1419 Size: 68 1170 181 Fragments arranged by size: 1170 181 68 SanDI GG'GwC_CC Cuts at: 0 1364 1419 Size: 1364 55 SexAI A'CCwGG_T Cuts at: 0 1203 1419 Size: 1203 216 SfcI C'TryA_G Cuts at: 0 337 891 1261 1419 Size: 337 554 370 158 Fragments arranged by size: 554 370 337 158 SmlI C'TyrA_G Cuts at: 0 67 151 1419 Size: 67 84 1268 Fragments arranged by size: 1268 84 67 SspI AAT'ATT Cuts at: 0 222 1090 1419 Size: 222 868 329 Fragments arranged by size: 868 329 222 StuI AGG'CCT Cuts at: 0 190 251 1354 1419 Size: 190 61 1103 65 Fragments arranged by size: 1103 190 65 61 StyI C'CwwG_G Cuts at: 0 59 786 1110 1116 1419 Size: 59 727 324 6 303 Fragments arranged by size: 727 324 303 59 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 99 411 856 1099 1419 Size: 99 312 445 243 320 Fragments arranged by size: 445 320 312 243 99 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 269 301 1419 Size: 269 32 1118 Fragments arranged by size: 1118 269 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 27 1419 Size: 27 1392 Enzymes that do cut: AarI AccI AflIII ApaI BanI BanII BbsI BcgI BciVI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FseI FspI HaeII Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SexAI SfcI SmlI SspI StuI StyI TaqII TstI XcmI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BsaHI BsaXI BseRI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI EagI EcoNI EcoRI EcoRV FalI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI