(Circular) (Six-base) MAPSORT of: pFN21AE4514.seq Check: 3775 from: 1 to: 6343 pFN21AE4514 6343 bp With 182 enzymes: SgfI * March 28, 2012 14:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4153 278 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2687 3686 1937 Size: 750 999 4594 Fragments arranged by size: 4594 999 750 Acc65I G'GTAC_C Cuts at: 3664 3664 Size: 6343 AclI AA'CG_TT Cuts at: 1563 3085 4470 4843 1563 Size: 1522 1385 373 3063 Fragments arranged by size: 3063 1522 1385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 2910 4406 5790 6058 1632 Size: 956 322 1496 1384 268 1917 Fragments arranged by size: 1917 1496 1384 956 322 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6146 AflIII A'CryG_T Cuts at: 1203 5242 6219 1203 Size: 4039 977 1327 Fragments arranged by size: 4039 1327 977 AgeI A'CCGG_T Cuts at: 5174 5174 Size: 6343 AhdI GACnn_n'nnGTC Cuts at: 5072 5072 Size: 6343 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6343 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3047 1184 Size: 1863 4480 AlwNI CAG_nnn'CTG Cuts at: 2460 4086 5658 2460 Size: 1626 1572 3145 Fragments arranged by size: 3145 1626 1572 ApaI G_GGCC'C Cuts at: 2347 2764 3409 3508 2347 Size: 417 645 99 5182 Fragments arranged by size: 5182 645 417 99 ApaLI G'TGCA_C Cuts at: 4400 5556 4400 Size: 1156 5187 ApoI r'AATT_y Cuts at: 1493 2429 3652 3878 4030 6139 1493 Size: 936 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 936 226 152 AseI AT'TA_AT Cuts at: 160 1032 4897 160 Size: 872 3865 1606 Fragments arranged by size: 3865 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 AvaI C'yCGr_G Cuts at: 1870 1943 2040 3668 1870 Size: 73 97 1628 4545 Fragments arranged by size: 4545 1628 97 73 BamHI G'GATC_C Cuts at: 2161 3673 4040 2161 Size: 1512 367 4464 Fragments arranged by size: 4464 1512 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2481 3664 5119 618 Size: 325 217 627 694 1183 1455 1842 Fragments arranged by size: 1842 1455 1183 694 627 325 217 BanII G_rGCy'C Cuts at: 1391 2105 2347 2764 3017 3327 3364 3409 3508 Size: 714 242 417 253 310 37 45 99 Cuts at: 3508 3662 1391 Size: 154 4072 Fragments arranged by size: 4072 714 417 310 253 242 154 99 45 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2333 928 Size: 971 434 4938 Fragments arranged by size: 4938 971 434 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4534 4568 1903 Size: 34 2597 34 3678 Fragments arranged by size: 3678 2597 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4235 5445 4235 Size: 1210 5133 BclI T'GATC_A Cuts at: 1548 1548 Size: 6343 BfrBI ATG'CAT Cuts at: 2244 2244 Size: 6343 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3021 4954 136 Size: 107 122 71 631 605 1349 1933 1525 Fragments arranged by size: 1933 1525 1349 631 605 122 107 71 BglII A'GATC_T Cuts at: 2416 3142 6338 2416 Size: 726 3196 2421 Fragments arranged by size: 3196 2421 726 BlpI GC'TnA_GC Cuts at: 3760 3760 Size: 6343 Bme1580I G_kGCm'C Cuts at: 1790 2347 2713 2764 3409 3508 4404 5560 1790 Size: 557 366 51 645 99 896 1156 2573 Fragments arranged by size: 2573 1156 896 645 557 366 99 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6343 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 3216 3466 Size: 416 53 83 399 24 1343 456 250 Cuts at: 3466 3485 4074 5032 442 Size: 19 589 958 1753 Fragments arranged by size: 1753 1343 958 589 456 416 399 250 83 53 24 19 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6343 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3220 3220 Size: 6343 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3031 5003 1239 Size: 1792 1972 2579 Fragments arranged by size: 2579 1972 1792 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4468 5333 5631 4468 Size: 865 298 5180 Fragments arranged by size: 5180 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2828 5006 882 Size: 617 1329 2178 2219 Fragments arranged by size: 2219 2178 1329 617 BsaAI yAC'GTr Cuts at: 493 1204 4134 493 Size: 711 2930 2702 Fragments arranged by size: 2930 2702 711 BsaBI GATnn'nnATC Cuts at: 6337 6337 Size: 6343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2594 4150 4532 5167 Size: 53 83 186 936 1061 1556 382 635 Cuts at: 5167 275 Size: 1451 Fragments arranged by size: 1556 1451 1061 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4776 5174 5448 5595 6006 4776 Size: 398 274 147 411 5113 Fragments arranged by size: 5113 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2216 2246 2663 2693 2856 Size: 30 165 30 813 30 417 30 163 Cuts at: 2856 2886 1178 Size: 30 4635 Fragments arranged by size: 4635 813 417 165 163 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2019 2877 3194 1186 Size: 833 858 317 4335 Fragments arranged by size: 4335 858 833 317 BseYI C'CCAG_C Cuts at: 2340 3363 3369 3521 5546 6042 2340 Size: 1023 6 152 2025 496 2641 Fragments arranged by size: 2641 2025 1023 496 152 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5770 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3793 4554 5582 1540 Size: 275 186 1792 761 1028 2301 Fragments arranged by size: 2301 1792 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2067 3327 4404 4489 5560 2012 Size: 55 1260 1077 85 1071 2795 Fragments arranged by size: 2795 1260 1077 1071 85 55 BsmI GAATG_Cn' Cuts at: 2652 3854 3947 2652 Size: 1202 93 5048 Fragments arranged by size: 5048 1202 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2601 2601 Size: 6343 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2067 2105 2347 2713 2764 3017 Size: 399 222 55 38 242 366 51 253 Cuts at: 3017 3327 3364 3409 3508 3662 4404 4489 5560 Size: 310 37 45 99 154 742 85 1071 Cuts at: 5560 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 366 310 253 242 222 154 99 85 55 51 45 38 37 BspEI T'CCGG_A Cuts at: 6006 6006 Size: 6343 BspHI T'CATG_A Cuts at: 3541 4232 5962 3541 Size: 691 1730 3922 Fragments arranged by size: 3922 1730 691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2889 3555 3698 844 Size: 2045 666 143 3489 Fragments arranged by size: 3489 2045 666 143 BsrBI CCG'CTC Cuts at: 1394 1823 4230 1394 Size: 429 2407 3507 Fragments arranged by size: 3507 2407 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4838 5012 65 Size: 1183 3590 174 1396 Fragments arranged by size: 3590 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2574 4987 5174 1700 Size: 110 764 2413 187 2869 Fragments arranged by size: 2869 2413 764 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6343 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6343 BssSI C'ACGA_G Cuts at: 4403 5415 4403 Size: 1012 5331 BstAPI GCAn_nnn'nTGC Cuts at: 794 2578 794 Size: 1784 4559 BstBI TT'CG_AA Cuts at: 5147 5147 Size: 6343 BstEII G'GTnAC_C Cuts at: 1184 2872 1184 Size: 1688 4655 BstYI r'GATC_y Cuts at: 1972 2161 2416 3142 3673 4040 4425 4442 5894 Size: 189 255 726 531 367 385 17 1452 Cuts at: 5894 5980 5992 6338 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 726 531 385 367 346 255 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2191 2377 2796 3207 513 Size: 552 1126 186 419 411 3649 Fragments arranged by size: 3649 1126 552 419 411 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5604 Fragments arranged by size: 5604 683 56 BtsI GCAGTG_nn' Cuts at: 2511 3859 4653 4673 2511 Size: 1348 794 20 4181 Fragments arranged by size: 4181 1348 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6343 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3117 3152 529 Size: 35 2553 35 3720 Fragments arranged by size: 3720 2553 35 35 DraI TTT'AAA Cuts at: 3647 3647 Size: 6343 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5350 809 Size: 507 4034 1802 Fragments arranged by size: 4034 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3790 4679 6308 Size: 54 1425 50 275 18 1960 889 1629 Cuts at: 6308 8 Size: 43 Fragments arranged by size: 1960 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3790 1537 Size: 275 1978 4090 Fragments arranged by size: 4090 1978 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2231 2301 2374 2704 4273 1353 Size: 878 70 73 330 1569 3423 Fragments arranged by size: 3423 1569 878 330 73 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4930 5304 5450 387 Size: 1064 138 3341 374 146 1280 Fragments arranged by size: 3341 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3325 3325 Size: 6343 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2588 2910 3031 4406 5003 5790 6058 Size: 393 956 322 121 1375 597 787 268 Cuts at: 6058 1239 Size: 1524 Fragments arranged by size: 1524 1375 956 787 597 393 322 268 121 EcoNI CCTnn'n_nnAGG Cuts at: 3597 3597 Size: 6343 EcoO109I rG'GnC_Cy Cuts at: 2344 2382 2761 3217 3405 3406 3589 2344 Size: 38 379 456 188 1 183 5098 Fragments arranged by size: 5098 456 379 188 183 38 1 EcoRI G'AATT_C Cuts at: 3652 4030 3652 Size: 378 5965 FspI TGC'GCA Cuts at: 4849 4849 Size: 6343 HaeII r_GCGC'y Cuts at: 1678 2995 5490 1678 Size: 1317 2495 2531 Fragments arranged by size: 2531 2495 1317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 2401 2433 2663 Size: 32 135 32 574 32 223 32 230 Cuts at: 2663 2695 4984 5016 5058 5090 1373 Size: 32 2289 32 42 32 2626 Fragments arranged by size: 2626 2289 574 230 223 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3687 3933 669 Size: 988 281 1749 246 3079 Fragments arranged by size: 3079 1749 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6343 HpaI GTT'AAC Cuts at: 3933 3933 Size: 6343 KpnI G_GTAC'C Cuts at: 3668 3668 Size: 6343 MfeI C'AATT_G Cuts at: 3942 3942 Size: 6343 MluI A'CGCG_T Cuts at: 6219 6219 Size: 6343 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3091 5457 5641 1305 Size: 1786 2366 184 2007 Fragments arranged by size: 2366 2007 1786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4521 Fragments arranged by size: 4521 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4301 4660 4819 518 Size: 648 3135 359 159 2042 Fragments arranged by size: 3135 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2457 2930 3454 3759 4436 5584 5829 1606 Size: 851 473 524 305 677 1148 245 2120 Fragments arranged by size: 2120 1148 851 677 524 473 305 245 NcoI C'CATG_G Cuts at: 513 1065 2191 2377 3207 513 Size: 552 1126 186 830 3649 Fragments arranged by size: 3649 1126 830 552 186 NdeI CA'TA_TG Cuts at: 387 387 Size: 6343 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6343 NotI GC'GGCC_GC Cuts at: 3790 3790 Size: 6343 NsiI A_TGCA'T Cuts at: 2246 2246 Size: 6343 NspI r_CATG'y Cuts at: 1132 1229 3701 5246 1132 Size: 97 2472 1545 2229 Fragments arranged by size: 2472 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2729 3599 1348 Size: 1381 870 4092 Fragments arranged by size: 4092 1381 870 PciI A'CATG_T Cuts at: 5242 5242 Size: 6343 PflMI CCAn_nnn'nTGG Cuts at: 1108 3213 3497 1108 Size: 2105 284 3954 Fragments arranged by size: 3954 2105 284 PmeI GTTT'AAAC Cuts at: 3647 3647 Size: 6343 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4412 4444 5925 5957 4412 Size: 32 1481 32 4798 Fragments arranged by size: 4798 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2382 3217 3589 2382 Size: 835 372 5136 Fragments arranged by size: 5136 835 372 PshAI GACnn'nnGTC Cuts at: 1613 2840 1613 Size: 1227 5116 PsiI TTA'TAA Cuts at: 3913 3913 Size: 6343 PspOMI G'GGCC_C Cuts at: 2343 2760 3405 3504 2343 Size: 417 645 99 5182 Fragments arranged by size: 5182 645 417 99 PstI C_TGCA'G Cuts at: 830 2583 3695 830 Size: 1753 1112 3478 Fragments arranged by size: 3478 1753 1112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6343 PvuII CAG'CTG Cuts at: 2457 2930 2457 Size: 473 5870 SacI G_AGCT'C Cuts at: 3327 3327 Size: 6343 SalI G'TCGA_C Cuts at: 1936 3685 1936 Size: 1749 4594 SanDI GG'GwC_CC Cuts at: 2382 3217 3589 2382 Size: 835 372 5136 Fragments arranged by size: 5136 835 372 SbfI CC_TGCA'GG Cuts at: 3695 3695 Size: 6343 ScaI AGT'ACT Cuts at: 4591 6303 4591 Size: 1712 4631 SfcI C'TryA_G Cuts at: 826 1045 2579 3691 4826 5507 5698 826 Size: 219 1534 1112 1135 681 191 1471 Fragments arranged by size: 1534 1471 1135 1112 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6343 SmaI CCC'GGG Cuts at: 1872 3670 1872 Size: 1798 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4447 5348 5610 820 Size: 197 926 2504 901 262 1553 Fragments arranged by size: 2504 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4134 493 Size: 3641 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6343 SphI G_CATG'C Cuts at: 1132 3701 1132 Size: 2569 3774 SspI AAT'ATT Cuts at: 5 52 4267 5 Size: 47 4215 2081 Fragments arranged by size: 4215 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6343 StyI C'CwwG_G Cuts at: 513 1065 2191 2377 3207 3260 3584 3782 513 Size: 552 1126 186 830 53 324 198 3074 Fragments arranged by size: 3074 1126 830 552 324 198 186 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2311 4370 4387 4540 4725 5170 325 Size: 1255 731 2059 17 153 185 445 1498 Fragments arranged by size: 2059 1498 1255 731 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3456 4589 6301 Size: 274 80 33 51 1443 1479 1133 1712 Cuts at: 6301 96 Size: 138 Fragments arranged by size: 1712 1479 1443 1133 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2216 2248 2856 2888 3370 3402 6303 6335 2216 Size: 32 608 32 482 32 2901 32 2224 Fragments arranged by size: 2901 2224 608 482 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2525 1316 Size: 1209 5134 XbaI T'CTAG_A Cuts at: 3679 3679 Size: 6343 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6343 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6343 XmaI C'CCGG_G Cuts at: 1870 3668 1870 Size: 1798 4545 XmnI GAAnn'nnTTC Cuts at: 4472 4472 Size: 6343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4151 276 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI