Restriction map for
pFN21AE6217
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
Acc65I AclI AfaI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BfaI BfrI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cac8I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MscI MseI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 352 GACGTC GACGT^C
AccI 385 GTCTAC GT^[AC][GT]AC
AccIII 593 TCCGGA T^CCGGA
AcsI 694 AAATTT [GA]^AATT[CT]
AflIII 515 ACATGT A^C[GA][CT]GT
AlwNI 708 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 297 CCTCAGG CC^T[ACGT]AGG
ApoI 694 AAATTT [GA]^AATT[CT]
BciVI 260 GTATCC GTATCC(6/5)
BglI 96 GCCGGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 506 AGATCT A^GATCT
BsaWI 593 TCCGGA [AT]^CCGG[AT]
BseAI 593 TCCGGA T^CCGGA
BspEI 593 TCCGGA T^CCGGA
BspLU11I 515 ACATGT A^CATGT
BstXI 264 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 297 CCTCAGG CC^T[ACGT]AGG
DraII 46 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 145 CCACGG C^C[GA][CT]GG
Eco81I 297 CCTCAGG CC^T[ACGT]AGG
EcoO109I 46 GGGGCCT [GA]G^G[ACGT]CC[CT]
HincII 334 GTTGAC GT[CT]^[GA]AC
HindII 334 GTTGAC GT[CT]^[GA]AC
HindIII 757 AAGCTT A^AGCTT
HphI 99 GGTGA GGTGA(8/7)
MaeIII 627 GTTAC ^GT[ACGT]AC
MroI 593 TCCGGA T^CCGGA
NlaIII 516 CATG CATG^
NspI 515 ACATGT [GA]CATG^[CT]
PflMI 20 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 784 GATGC GCATC(5/9)
SmlI 754 CTCAAG C^T[CT][GA]AG
TaqI 578 TCGA T^CGA
Tsp509I 695 AATT ^AATT
TthHB8I 578 TCGA T^CGA
Van91I 20 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 34 CGCG CG^CG
128 CGCG
BamHI 419 GGATCC G^GATCC
557 GGATCC
BbsI 429 GAAGAC GAAGAC(2/6)
526 GTCTTC
BcnI 116 CCGGG CC^[GC]GG
744 CCCGG
BpuAI 429 GAAGAC GAAGAC(2/6)
526 GTCTTC
BsrFI 96 GCCGGT [GA]^CCGG[CT]
666 GCCGGT
BstUI 34 CGCG CG^CG
128 CGCG
Cfr10I 96 GCCGGT [GA]^CCGG[CT]
666 GCCGGT
HaeII 54 GGCGCT [GA]GCGC^[CT]
112 AGCGCC
HgaI 450 GACGC GACGC(5/10)
275 GCGTC
MaeII 353 ACGT A^CGT
630 ACGT
MboII 429 GAAGA GAAGA(8/7)
527 TCTTC
MvnI 34 CGCG CG^CG
128 CGCG
NciI 116 CCGGG CC^[GC]GG
744 CCCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcyI AluI AlwI BbvI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstYI CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI MboI MflI MnlI
MslI MspI MvaI MwoI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
TseI TspRI XhoII