(Circular) (Six-base) MAPSORT of: pFN21AE9689.seq Check: 2214 from: 1 to: 6175 pFN21AE9689 6175 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 2817 4302 4675 1563 Size: 1254 1485 373 3063 Fragments arranged by size: 3063 1485 1254 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3194 3344 4238 5622 5890 1632 Size: 1562 150 894 1384 268 1917 Fragments arranged by size: 1917 1562 1384 894 268 150 AflII C'TTAA_G Cuts at: 820 1017 3319 820 Size: 197 2302 3676 Fragments arranged by size: 3676 2302 197 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2535 1184 Size: 1351 4824 AlwNI CAG_nnn'CTG Cuts at: 2759 3918 5490 2759 Size: 1159 1572 3444 Fragments arranged by size: 3444 1572 1159 ApaLI G'TGCA_C Cuts at: 3339 4232 5388 3339 Size: 893 1156 4126 Fragments arranged by size: 4126 1156 893 ApoI r'AATT_y Cuts at: 1493 2008 2543 3461 3484 3710 3862 5971 1493 Size: 515 535 918 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 918 535 515 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2400 3500 1870 Size: 73 457 1100 4545 Fragments arranged by size: 4545 1100 457 73 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3496 4951 618 Size: 325 217 627 1709 1455 1842 Fragments arranged by size: 1842 1709 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2332 2401 3494 1391 Size: 941 69 1093 4072 Fragments arranged by size: 4072 1093 941 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2817 928 Size: 971 918 4286 Fragments arranged by size: 4286 971 918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2150 2184 4366 4400 1903 Size: 34 213 34 2182 34 3678 Fragments arranged by size: 3678 2182 213 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2611 4067 5277 2611 Size: 1456 1210 3509 Fragments arranged by size: 3509 1456 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2638 6170 2638 Size: 3532 2643 BlpI GC'TnA_GC Cuts at: 3165 3592 3165 Size: 427 5748 Bme1580I G_kGCm'C Cuts at: 1790 3343 4236 5392 1790 Size: 1553 893 1156 2573 Fragments arranged by size: 2573 1553 1156 893 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3906 4864 442 Size: 416 53 83 399 24 2489 958 1753 Fragments arranged by size: 2489 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2777 1056 Size: 1721 4454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 4835 1239 Size: 993 2603 2579 Fragments arranged by size: 2603 2579 993 Bpu10I CC'TnA_GC Cuts at: 2663 2663 Size: 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 2568 6169 2568 Size: 3601 2574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2347 3982 4364 4999 Size: 53 83 186 936 814 1635 382 635 Cuts at: 4999 275 Size: 1451 Fragments arranged by size: 1635 1451 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5950 Fragments arranged by size: 5950 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3039 1186 Size: 1853 4322 BseYI C'CCAG_C Cuts at: 5378 5874 5378 Size: 496 5679 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5602 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2332 2422 3343 4236 4321 5392 2332 Size: 90 921 893 85 1071 3115 Fragments arranged by size: 3115 1071 921 893 90 85 BsmI GAATG_Cn' Cuts at: 2616 3686 3779 2616 Size: 1070 93 5012 Fragments arranged by size: 5012 1070 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3295 3295 Size: 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2332 2401 2422 3343 3494 4236 4321 Size: 399 542 69 21 921 151 742 85 Cuts at: 4321 5392 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 921 742 542 399 151 85 69 21 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3530 844 Size: 2686 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2119 3303 4670 4844 65 Size: 1183 871 1184 1367 174 1396 Fragments arranged by size: 1396 1367 1184 1183 871 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6175 BstYI r'GATC_y Cuts at: 1972 2137 2287 2569 2638 3202 3467 3505 3872 Size: 165 150 282 69 564 265 38 367 Cuts at: 3872 4257 4274 5726 5812 5824 6170 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 564 385 367 346 282 265 165 150 86 69 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2723 3086 513 Size: 552 1658 363 3602 Fragments arranged by size: 3602 1658 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 3691 4485 4505 3691 Size: 794 20 5361 Fragments arranged by size: 5361 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 2233 3479 2233 Size: 1246 4929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2300 3622 4511 Size: 54 1425 50 275 18 470 1322 889 Cuts at: 4511 6140 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1322 889 470 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2038 2944 3154 4105 1353 Size: 685 906 210 951 3423 Fragments arranged by size: 3423 951 906 685 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4762 5136 5282 387 Size: 1064 138 3173 374 146 1280 Fragments arranged by size: 3173 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2330 2330 Size: 6175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 3194 3344 4238 4835 5622 5890 Size: 393 600 962 150 894 597 787 268 Cuts at: 5890 1239 Size: 1524 Fragments arranged by size: 1524 962 894 787 600 597 393 268 150 EcoNI CCTnn'n_nnAGG Cuts at: 3188 3188 Size: 6175 EcoO109I rG'GnC_Cy Cuts at: 2405 2405 Size: 6175 EcoRI G'AATT_C Cuts at: 3484 3862 3484 Size: 378 5797 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3374 3374 Size: 6175 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2332 2364 2585 2617 3294 Size: 32 135 32 760 32 221 32 677 Cuts at: 3294 3326 4816 4848 4890 4922 1373 Size: 32 1490 32 42 32 2626 Fragments arranged by size: 2626 1490 760 677 221 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3765 669 Size: 988 281 1581 246 3079 Fragments arranged by size: 3079 1581 988 281 246 HindIII A'AGCT_T Cuts at: 748 3102 748 Size: 2354 3821 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2725 3001 5289 5473 1305 Size: 1420 276 2288 184 2007 Fragments arranged by size: 2288 2007 1420 276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2302 10 Size: 54 1425 343 470 3883 Fragments arranged by size: 3883 1425 470 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2625 3008 4133 4492 4651 518 Size: 648 1459 383 1125 359 159 2042 Fragments arranged by size: 2042 1459 1125 648 383 359 159 MspA1I CmG'CkG Cuts at: 1606 3591 4268 5416 5661 1606 Size: 1985 677 1148 245 2120 Fragments arranged by size: 2120 1985 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2723 513 Size: 552 1658 3965 Fragments arranged by size: 3965 1658 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 2773 1052 Size: 1721 4454 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 3533 5078 1132 Size: 97 2304 1545 2229 Fragments arranged by size: 2304 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 2759 1108 Size: 1651 4524 PfoI T'CCnGG_A Cuts at: 2603 2603 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2405 2405 Size: 6175 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PstI C_TGCA'G Cuts at: 830 2320 2945 3430 3527 830 Size: 1490 625 485 97 3478 Fragments arranged by size: 3478 1490 625 485 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 SacI G_AGCT'C Cuts at: 2332 2332 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SapI GCTCTTCn'nnn_ Cuts at: 3154 3154 Size: 6175 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2316 2409 2470 2941 3426 3523 4658 Size: 219 1271 93 61 471 485 97 1135 Cuts at: 4658 5339 5530 826 Size: 681 191 1471 Fragments arranged by size: 1471 1271 1135 681 485 471 219 191 97 93 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 2402 3502 1872 Size: 530 1100 4545 Fragments arranged by size: 4545 1100 530 SmlI C'TyrA_G Cuts at: 820 1017 1943 3319 4279 5180 5442 820 Size: 197 926 1376 960 901 262 1553 Fragments arranged by size: 1553 1376 960 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2723 3614 513 Size: 552 1658 891 3074 Fragments arranged by size: 3074 1658 891 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4202 4219 4372 4557 5002 325 Size: 1255 2622 17 153 185 445 1498 Fragments arranged by size: 2622 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2734 4421 6133 Size: 274 80 33 51 1443 757 1687 1712 Cuts at: 6133 96 Size: 138 Fragments arranged by size: 1712 1687 1443 757 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6135 6167 6135 Size: 32 6143 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3058 3058 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 2400 3500 1870 Size: 530 1100 4545 Fragments arranged by size: 4545 1100 530 XmnI GAAnn'nnTTC Cuts at: 4304 4304 Size: 6175 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI