Restriction map for
pFN21AB6376
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 370 GAATTC [GA]^AATT[CT]
AfaI 564 GTAC GT^AC
AlwI 756 GGATC GGATC(4/5)
ApoI 370 GAATTC [GA]^AATT[CT]
Asp700I 204 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 71 GTGCTC G[AT]GC[AT]^C
BanI 868 GGTGCC G^G[CT][GA]CC
BcgI 609 GCAGAGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 594 GTATCC GTATCC(6/5)
BclI 890 TGATCA T^GATCA
BfaI 478 CTAG C^TAG
BsaI 703 GAGACC GGTCTC(1/5)
BseRI 55 GAGGAG GAGGAG(10/8)
BsiHKAI 71 GTGCTC G[AT]GC[AT]^C
BsrDI 268 GCAATG GCAATG(2/0)
BstXI 461 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 756 GGATCT [GA]^GATC[CT]
Cac8I 801 GCTGGC GC[ACGT]^[ACGT]GC
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoRI 370 GAATTC G^AATTC
FbaI 890 TGATCA T^GATCA
HgaI 668 GCGTC GACGC(5/10)
HphI 877 GGTGA GGTGA(8/7)
MaeI 478 CTAG C^TAG
MflI 756 GGATCT [GA]^GATC[CT]
MspA1I 8 CCGCTG C[AC]G^C[GT]G
NcoI 467 CCATGG C^CATGG
NspI 440 GCATGT [GA]CATG^[CT]
PflMI 297 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 612 GAGTC GAGTC(4/5)
PstI 332 CTGCAG CTGCA^G
RsaI 564 GTAC GT^AC
SapI 451 GAAGAGC GCTCTTC(1/4)
TfiI 622 GAATC G^A[AT]TC
Van91I 297 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 756 GGATCT [GA]^GATC[CT]
XmnI 204 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 326 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
883 CAGTGTCTG
ApaI 145 GGGCCC GGGCC^C
858 GGGCCC
BanII 145 GGGCCC G[GA]GC[CT]^C
858 GGGCCC
BbvCI 10 GCTGAGG CCTCAGC(-5/-2)
283 GCTGAGG
BsiYI 297 CCACCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
467 CCATGGAGGGG
BslI 297 CCACCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
467 CCATGGAGGGG
BsmAI 167 GAGAC GTCTC(1/5)
703 GAGAC
BsrI 234 ACTGG ACTGG(1/-1)
872 CCAGT
DraII 144 GGGGCCC [GA]G^G[ACGT]CC[CT]
145 GGGCCCC
DsaI 125 CCACGG C^C[GA][CT]GG
467 CCATGG
EcoO109I 144 GGGGCCC [GA]G^G[ACGT]CC[CT]
145 GGGCCCC
HinfI 612 GAGTC G^A[ACGT]TC
622 GAATC
SexAI 299 ACCTGGT A^CC[AT]GGT
864 ACCAGGT
SfcI 21 CTGTAG C^T[GA][CT]AG
332 CTGCAG
Tsp509I 50 AATT ^AATT
371 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BsaJI Bsp1286I BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraIII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
ItaI Ksp632I MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I