(Circular) (Six-base) MAPSORT of: pFN21AB6376.seq Check: 1825 from: 1 to: 5602 pFN21AB6376 5602 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 2600 3665 5049 5317 1632 Size: 785 183 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 785 268 183 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2537 2569 2537 Size: 32 5570 AlwNI CAG_nnn'CTG Cuts at: 2336 2893 3345 4917 2336 Size: 557 452 1572 3021 Fragments arranged by size: 3021 1572 557 452 ApaI G_GGCC'C Cuts at: 2154 2867 2154 Size: 713 4889 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2375 2911 3137 3289 5398 1493 Size: 882 536 226 152 2109 1697 Fragments arranged by size: 2109 1697 882 536 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2927 1870 Size: 73 984 4545 Fragments arranged by size: 4545 984 73 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2873 2923 4378 618 Size: 325 217 627 1086 50 1455 1842 Fragments arranged by size: 1842 1455 1086 627 325 217 50 BanII G_rGCy'C Cuts at: 1391 2154 2867 2921 1391 Size: 763 713 54 4072 Fragments arranged by size: 4072 763 713 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BbvCI CC'TCA_GC Cuts at: 2016 2289 2016 Size: 273 5329 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2603 2637 3793 3827 1903 Size: 34 666 34 1156 34 3678 Fragments arranged by size: 3678 1156 666 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2610 3494 4704 2610 Size: 884 1210 3508 Fragments arranged by size: 3508 1210 884 BclI T'GATC_A Cuts at: 1548 2895 1548 Size: 1347 4255 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4213 136 Size: 107 122 71 631 605 2541 1525 Fragments arranged by size: 2541 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 2154 2867 3663 4819 1790 Size: 364 713 796 1156 2573 Fragments arranged by size: 2573 1156 796 713 364 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3333 4291 442 Size: 416 53 83 399 24 1916 958 1753 Fragments arranged by size: 1916 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2425 2622 2832 4262 1239 Size: 1186 197 210 1430 2579 Fragments arranged by size: 2579 1430 1186 210 197 Bpu10I CC'TnA_GC Cuts at: 2016 2289 2016 Size: 273 5329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2702 4265 882 Size: 617 1203 1563 2219 Fragments arranged by size: 2219 1563 1203 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 3791 4426 275 Size: 53 83 186 936 1876 382 635 1451 Fragments arranged by size: 1876 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 1178 Size: 30 165 30 1134 30 4213 Fragments arranged by size: 4213 1134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 1186 Size: 889 4713 BseYI C'CCAG_C Cuts at: 2110 4805 5301 2110 Size: 2695 496 2411 Fragments arranged by size: 2695 2411 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5029 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2080 3663 3748 4819 2080 Size: 1583 85 1071 2863 Fragments arranged by size: 2863 1583 1071 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2154 2867 2921 3663 3748 4819 Size: 399 290 74 713 54 742 85 1071 Cuts at: 4819 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 713 399 290 85 74 54 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 844 Size: 2113 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2280 4097 4271 65 Size: 1183 1032 1817 174 1396 Fragments arranged by size: 1817 1396 1183 1032 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2473 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2761 2932 3299 3684 3701 5153 5239 5251 Size: 789 171 367 385 17 1452 86 12 Cuts at: 5251 5597 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 789 385 367 346 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2130 2472 513 Size: 552 939 126 342 3643 Fragments arranged by size: 3643 939 552 342 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 2175 2253 2359 3118 3912 3932 2175 Size: 78 106 759 794 20 3845 Fragments arranged by size: 3845 794 759 106 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DraIII CAC_nnn'GTG Cuts at: 2308 2405 2873 2308 Size: 97 468 5037 Fragments arranged by size: 5037 468 97 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3049 3938 Size: 54 1425 50 275 18 177 1042 889 Cuts at: 3938 5567 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1042 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2198 2451 3532 1353 Size: 800 45 253 1081 3423 Fragments arranged by size: 3423 1081 800 253 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2425 2600 2622 2832 3665 4262 Size: 393 785 8 175 22 210 833 597 Cuts at: 4262 5049 5317 1239 Size: 787 268 1524 Fragments arranged by size: 1524 833 787 785 597 393 268 210 175 22 8 EcoO109I rG'GnC_Cy Cuts at: 2150 2151 2150 Size: 1 5601 EcoRI G'AATT_C Cuts at: 2375 2911 3289 2375 Size: 536 378 4688 Fragments arranged by size: 4688 536 378 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 4749 1678 Size: 3071 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 4243 4275 4317 Size: 32 135 32 776 32 1863 32 42 Cuts at: 4317 4349 1373 Size: 32 2626 Fragments arranged by size: 2626 1863 776 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 4716 4900 1305 Size: 1125 2286 184 2007 Fragments arranged by size: 2286 2007 1125 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3780 Fragments arranged by size: 3780 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3560 3919 4078 518 Size: 648 2394 359 159 2042 Fragments arranged by size: 2394 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 3018 3695 4843 5088 1606 Size: 409 1003 677 1148 245 2120 Fragments arranged by size: 2120 1148 1003 677 409 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2472 513 Size: 552 939 468 3643 Fragments arranged by size: 3643 939 552 468 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2449 2960 4505 1132 Size: 97 1220 511 1545 2229 Fragments arranged by size: 2229 1545 1220 511 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2308 1108 Size: 1200 4402 PfoI T'CCnGG_A Cuts at: 2217 2217 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2569 3671 3703 5184 5216 2537 Size: 32 1102 32 1481 32 2923 Fragments arranged by size: 2923 1481 1102 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PspOMI G'GGCC_C Cuts at: 2150 2863 2150 Size: 713 4889 PstI C_TGCA'G Cuts at: 830 2341 2954 830 Size: 1511 613 3478 Fragments arranged by size: 3478 1511 613 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SapI GCTCTTCn'nnn_ Cuts at: 2451 2451 Size: 5602 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SexAI A'CCwGG_T Cuts at: 2304 2869 2304 Size: 565 5037 SfcI C'TryA_G Cuts at: 826 1045 2026 2337 2950 4085 4766 4957 826 Size: 219 981 311 613 1135 681 191 1471 Fragments arranged by size: 1471 1135 981 681 613 311 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2929 1872 Size: 1057 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2004 2466 2472 2556 3041 513 Size: 552 939 462 6 84 485 3074 Fragments arranged by size: 3074 939 552 485 462 84 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3848 5560 96 Size: 274 80 33 51 1443 1871 1712 138 Fragments arranged by size: 1871 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2927 1870 Size: 1057 4545 XmnI GAAnn'nnTTC Cuts at: 2213 3731 2213 Size: 1518 4084 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI