################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraII DraIII DsaI Eco47III EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 107 GGTACC G^GTACC AccI 85 GTCTAC GT^[AC][GT]AC AcyI 77 GACGCC G[GA]^CG[CT]C AflIII 152 ACACGT A^C[GA][CT]GT AhdI 77 GACGCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 107 GGTACC G^GTACC AspEI 77 GACGCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 517 GAGCTC G[AT]GC[AT]^C BalI 50 TGGCCA TGG^CCA BamHI 1182 GGATCC G^GATCC BbsI 790 GAAGAC GAAGAC(2/6) BglI 463 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 790 GAAGAC GAAGAC(2/6) BsaAI 46 TACGTG [CT]AC^GT[GA] BsaHI 77 GACGCC G[GA]^CG[CT]C BsiHKAI 517 GAGCTC G[AT]GC[AT]^C BsmI 832 GCATTC GAATGC(1/-1) BspMI 110 ACCTGC ACCTGC(4/8) BsrBI 473 CCGCTC CCGCTC(-3/-3) BsrFI 1037 ACCGGC [GA]^CCGG[CT] BstAPI 823 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1182 GGATCC [GA]^GATC[CT] Cfr10I 1037 ACCGGC [GA]^CCGG[CT] DrdI 1324 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 77 GACGCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1265 CTCTTC CTCTTC(1/4) EcoRI 13 GAATTC G^AATTC Hin1I 77 GACGCC G[GA]^CG[CT]C KpnI 107 GGTACC GGTAC^C Ksp632I 1265 CTCTTC CTCTTC(1/4) MflI 1182 GGATCC [GA]^GATC[CT] MluNI 50 TGGCCA TGG^CCA MscI 50 TGGCCA TGG^CCA NdeI 795 CATATG CA^TATG NotI 114 GCGGCCGC GC^GGCCGC PvuII 1147 CAGCTG CAG^CTG SacI 517 GAGCTC GAGCT^C SapI 1264 GCTCTTC GCTCTTC(1/4) SbfI 663 CCTGCAGG CCTGCA^GG SfiI 462 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 103 CCCGGG CCC^GGG SphI 328 GCATGC GCATG^C Sse8387I 663 CCTGCAGG CCTGCA^GG XhoII 1182 GGATCC [GA]^GATC[CT] XmaCI 103 CCCGGG C^CCGGG XmaI 103 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 13 GAATTC [GA]^AATT[CT] 921 GAATTT AfaI 108 GTAC GT^AC 255 GTAC AocI 139 CCTCAGG CC^T[ACGT]AGG 1317 CCTCAGG ApoI 13 GAATTC [GA]^AATT[CT] 921 GAATTT Asp700I 1207 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1261 GAAGCTCTTC AvaI 103 CCCGGG C^[CT]CG[GA]G 364 CTCGGG BanI 107 GGTACC G^G[CT][GA]CC 854 GGCACC BanII 100 GAGCCC G[GA]GC[CT]^C 517 GAGCTC BmyI 100 GAGCCC G[AGT]GC[ACT]^C 517 GAGCTC BseRI 576 CTCCTC GAGGAG(10/8) 422 GAGGAG BsgI 802 CTGCAC GTGCAG(16/14) 1378 GTGCAG BsiEI 115 CGGCCG CG[GA][CT]^CG 1412 CGGCCG BsmAI 343 GAGAC GTCTC(1/5) 683 GAGAC BsoBI 103 CCCGGG C^[CT]CG[GA]G 364 CTCGGG Bsp1286I 100 GAGCCC G[AGT]GC[ACT]^C 517 GAGCTC BstXI 53 CCAACTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 452 CCACCCTGGTGG Bsu36I 139 CCTCAGG CC^T[ACGT]AGG 1317 CCTCAGG CfoI 1176 GCGC GCG^C 1338 GCGC EagI 115 CGGCCG C^GGCCG 1412 CGGCCG EclXI 115 CGGCCG C^GGCCG 1412 CGGCCG Eco52I 115 CGGCCG C^GGCCG 1412 CGGCCG Eco81I 139 CCTCAGG CC^T[ACGT]AGG 1317 CCTCAGG HhaI 1176 GCGC GCG^C 1338 GCGC HinP1I 1176 GCGC G^CGC 1338 GCGC PleI 1109 GAGTC GAGTC(4/5) 1165 GAGTC RsaI 108 GTAC GT^AC 255 GTAC XmnI 1207 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1261 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI