(Circular) (Six-base) MAPSORT of: pFN21AE4463.seq Check: 7977 from: 1 to: 6145 pFN21AE4463 6145 bp With 182 enzymes: SgfI * March 28, 2012 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2091 3488 1937 Size: 154 1397 4594 Fragments arranged by size: 4594 1397 154 Acc65I G'GTAC_C Cuts at: 2112 3466 2112 Size: 1354 4791 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3023 4208 5592 5860 1632 Size: 1391 1185 1384 268 1917 Fragments arranged by size: 1917 1391 1384 1185 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 2157 5044 6021 1203 Size: 954 2887 977 1327 Fragments arranged by size: 2887 1327 977 954 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 2087 4874 2087 Size: 2787 3358 AleI CACnn'nnGTG Cuts at: 1166 2462 1166 Size: 1296 4849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2832 2860 1184 Size: 1648 28 4469 Fragments arranged by size: 4469 1648 28 AlwNI CAG_nnn'CTG Cuts at: 3888 5460 3888 Size: 1572 4573 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 2018 2926 3454 3680 3832 5941 1493 Size: 525 908 528 226 152 2109 1697 Fragments arranged by size: 2109 1697 908 528 525 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2369 3470 1870 Size: 73 165 261 1101 4545 Fragments arranged by size: 4545 1101 261 165 73 BamHI G'GATC_C Cuts at: 3187 3475 3842 3187 Size: 288 367 5490 Fragments arranged by size: 5490 367 288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 2859 3466 4921 618 Size: 325 217 627 325 747 607 1455 1842 Fragments arranged by size: 1842 1455 747 627 607 325 325 217 BanII G_rGCy'C Cuts at: 1391 2109 2526 3464 1391 Size: 718 417 938 4072 Fragments arranged by size: 4072 938 718 417 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2802 928 Size: 971 903 4271 Fragments arranged by size: 4271 971 903 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2474 4756 136 Size: 107 122 71 631 605 802 2282 1525 Fragments arranged by size: 2282 1525 802 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 4206 5362 1790 Size: 2416 1156 2573 Fragments arranged by size: 2573 2416 1156 BmgBI CAC'GTC Cuts at: 1318 2101 3424 1318 Size: 783 1323 4039 Fragments arranged by size: 4039 1323 783 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3071 3876 4834 Size: 416 53 83 399 24 1654 805 958 Cuts at: 4834 442 Size: 1753 Fragments arranged by size: 1753 1654 958 805 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2436 2702 4805 1239 Size: 1127 70 266 2103 2579 Fragments arranged by size: 2579 2103 1127 266 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2053 3936 493 Size: 711 849 1883 2702 Fragments arranged by size: 2702 1883 849 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 3952 4334 4969 Size: 53 83 186 936 550 1869 382 635 Cuts at: 4969 275 Size: 1451 Fragments arranged by size: 1869 1451 936 635 550 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 1178 Size: 30 165 30 693 30 5197 Fragments arranged by size: 5197 693 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2442 2572 1186 Size: 1256 130 4759 Fragments arranged by size: 4759 1256 130 BseYI C'CCAG_C Cuts at: 2329 2597 5348 5844 2329 Size: 268 2751 496 2630 Fragments arranged by size: 2751 2630 496 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2792 3404 1163 Size: 573 1056 612 3904 Fragments arranged by size: 3904 1056 612 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2123 3420 3595 4356 5384 1540 Size: 275 186 122 1297 175 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 175 122 BsiHKAI G_wGCw'C Cuts at: 2526 4206 4291 5362 2526 Size: 1680 85 1071 3309 Fragments arranged by size: 3309 1680 1071 85 BsmI GAATG_Cn' Cuts at: 2837 3656 3749 2837 Size: 819 93 5233 Fragments arranged by size: 5233 819 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2526 3464 4206 4291 5362 1391 Size: 399 319 417 938 742 85 1071 2174 Fragments arranged by size: 2174 1071 938 742 417 399 319 85 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 3500 844 Size: 1280 1376 3489 Fragments arranged by size: 3489 1376 1280 BsrBI CCG'CTC Cuts at: 1394 1823 2480 4032 1394 Size: 429 657 1552 3507 Fragments arranged by size: 3507 1552 657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4640 4814 65 Size: 1183 3392 174 1396 Fragments arranged by size: 3392 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3042 4789 4976 1700 Size: 110 1232 1747 187 2869 Fragments arranged by size: 2869 1747 1232 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 2834 794 Size: 2040 4105 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstXI CCAn_nnnn'nTGG Cuts at: 2065 2464 2065 Size: 399 5746 BstYI r'GATC_y Cuts at: 1972 3187 3475 3842 4227 4244 5696 5782 5794 Size: 1215 288 367 385 17 1452 86 12 Cuts at: 5794 6140 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1215 385 367 346 288 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2145 3323 2145 Size: 1178 4967 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5593 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 2247 2839 3661 4455 4475 2247 Size: 592 822 794 20 3917 Fragments arranged by size: 3917 822 794 592 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3335 5152 809 Size: 507 2019 1817 1802 Fragments arranged by size: 2019 1817 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2120 2265 Size: 54 1425 50 275 18 225 65 145 Cuts at: 2265 2475 3417 3592 4481 6110 8 Size: 210 942 175 889 1629 43 Fragments arranged by size: 1629 1425 942 889 275 225 210 175 145 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2120 3417 3592 1537 Size: 275 308 1297 175 4090 Fragments arranged by size: 4090 1297 308 275 175 EarI CTCTTCn'nnn_ Cuts at: 1353 3276 4075 1353 Size: 1923 799 3423 Fragments arranged by size: 3423 1923 799 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2524 2524 Size: 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2436 2702 3023 4208 4805 5592 Size: 393 734 70 266 321 1185 597 787 Cuts at: 5592 5860 1239 Size: 268 1524 Fragments arranged by size: 1524 1185 787 734 597 393 321 268 266 70 EcoRI G'AATT_C Cuts at: 2018 3454 3832 2018 Size: 1436 378 4331 Fragments arranged by size: 4331 1436 378 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 5292 1678 Size: 3614 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 2418 2450 3155 Size: 32 135 32 501 32 313 32 705 Cuts at: 3155 3187 4786 4818 4860 4892 1373 Size: 32 1599 32 42 32 2626 Fragments arranged by size: 2626 1599 705 501 313 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 2116 3470 2116 Size: 1354 4791 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3276 5259 5443 1305 Size: 1971 1983 184 2007 Fragments arranged by size: 2007 1983 1971 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 10 Size: 54 1425 343 225 4098 Fragments arranged by size: 4098 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 2814 2948 4103 4462 4621 518 Size: 648 1296 352 134 1155 359 159 2042 Fragments arranged by size: 2042 1296 1155 648 359 352 159 134 MspA1I CmG'CkG Cuts at: 1606 2125 2138 3154 3388 3561 4238 5386 5631 Size: 519 13 1016 234 173 677 1148 245 Cuts at: 5631 1606 Size: 2120 Fragments arranged by size: 2120 1148 1016 677 519 245 234 173 13 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5593 NdeI CA'TA_TG Cuts at: 387 2801 387 Size: 2414 3731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 2120 3592 2120 Size: 1472 4673 NspI r_CATG'y Cuts at: 1132 1229 2284 2337 2748 3149 3503 5048 1132 Size: 97 1055 53 411 401 354 1545 2229 Fragments arranged by size: 2229 1545 1055 411 401 354 97 53 PasI CC'CwG_GG Cuts at: 1348 2731 1348 Size: 1383 4762 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PstI C_TGCA'G Cuts at: 830 2508 2673 3129 3497 830 Size: 1678 165 456 368 3478 Fragments arranged by size: 3478 1678 456 368 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 3154 3154 Size: 6145 SacI G_AGCT'C Cuts at: 2526 2526 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SapI GCTCTTCn'nnn_ Cuts at: 3276 3276 Size: 6145 SbfI CC_TGCA'GG Cuts at: 2673 3497 2673 Size: 824 5321 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SfcI C'TryA_G Cuts at: 826 1045 2504 2669 3125 3493 4628 5309 5500 Size: 219 1459 165 456 368 1135 681 191 Cuts at: 5500 826 Size: 1471 Fragments arranged by size: 1471 1459 1135 681 456 368 219 191 165 SfiI GGCCn_nnn'nGGCC Cuts at: 2474 2474 Size: 6145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2110 3472 1872 Size: 238 1362 4545 Fragments arranged by size: 4545 1362 238 SmlI C'TyrA_G Cuts at: 820 1017 1943 4249 5150 5412 820 Size: 197 926 2306 901 262 1553 Fragments arranged by size: 2306 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 2337 3503 1132 Size: 1205 1166 3774 Fragments arranged by size: 3774 1205 1166 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 3584 513 Size: 552 2519 3074 Fragments arranged by size: 3074 2519 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2765 2938 4172 4189 4342 4527 4972 Size: 1255 1185 173 1234 17 153 185 445 Cuts at: 4972 325 Size: 1498 Fragments arranged by size: 1498 1255 1234 1185 445 185 173 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4391 6103 96 Size: 274 80 33 51 1443 2414 1712 138 Fragments arranged by size: 2414 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 6105 6137 2270 Size: 32 3803 32 2278 Fragments arranged by size: 3803 2278 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 2108 3470 1870 Size: 238 1362 4545 Fragments arranged by size: 4545 1362 238 XmnI GAAnn'nnTTC Cuts at: 3216 3270 4274 3216 Size: 54 1004 5087 Fragments arranged by size: 5087 1004 54 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI