Restriction map for
pFN21AB3606
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MboII MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
TfiI TseI Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwI 257 GGATC GGATC(4/5)
AlwNI 32 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 288 GGGCCC GGGCC^C
BanI 67 GGCGCC G^G[CT][GA]CC
BanII 288 GGGCCC G[GA]GC[CT]^C
BbeI 67 GGCGCC GGCGC^C
BcnI 269 CCCGG CC^[GC]GG
BsaAI 109 TACGTG [CT]AC^GT[GA]
BsaWI 7 TCCGGT [AT]^CCGG[AT]
BsgI 182 GTGCAG GTGCAG(16/14)
BsmAI 223 GTCTC GTCTC(1/5)
BsmBI 222 CGTCTC CGTCTC(1/5)
BspDI 394 ATCGAT AT^CGAT
BsrDI 369 CATTGC GCAATG(2/0)
BsrI 486 CCAGT ACTGG(1/-1)
BssHII 16 GCGCGC G^CGCGC
BstXI 50 CCACAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 394 ATCGAT AT^CGAT
DsaI 452 CCGTGG C^C[GA][CT]GG
FauI 517 CCCGC CCCGC(4/6)
Fnu4HI 468 GCGGC GC^[ACGT]GC
HaeII 67 GGCGCC [GA]GCGC^[CT]
HinfI 232 GACTC G^A[ACGT]TC
ItaI 468 GCGGC GC^[ACGT]GC
KasI 67 GGCGCC G^GCGCC
MwoI 197 GCAAGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 67 GGCGCC GG^CGCC
NciI 269 CCCGG CC^[GC]GG
NlaIII 502 CATG CATG^
PleI 232 GACTC GAGTC(4/5)
PshAI 326 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 20 GCATC GCATC(5/9)
SfcI 375 CTACAG C^T[GA][CT]AG
SfoI 67 GGCGCC GGC^GCC
SspI 575 AATATT AAT^ATT
StuI 479 AGGCCT AGG^CCT
TaqI 395 TCGA T^CGA
TthHB8I 395 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 67 GGCGCC G[GA]^CG[CT]C
220 GGCGTC
AfaI 108 GTAC GT^AC
489 GTAC
AluI 86 AGCT AG^CT
200 AGCT
AvaII 114 GGTCC G^G[AT]CC
474 GGTCC
BmyI 150 GTGCCC G[AGT]GC[ACT]^C
288 GGGCCC
BsaHI 67 GGCGCC G[GA]^CG[CT]C
220 GGCGTC
BsaJI 268 CCCCGG C^C[ACGT][ACGT]GG
452 CCGTGG
BsmFI 444 GGGAC GGGAC(10/14)
515 GTCCC
Bsp1286I 150 GTGCCC G[AGT]GC[ACT]^C
288 GGGCCC
DdeI 236 CTCAG C^T[ACGT]AG
335 CTGAG
DraII 288 GGGCCCC [GA]G^G[ACGT]CC[CT]
380 GGGGCCT
EcoO109I 288 GGGCCCC [GA]G^G[ACGT]CC[CT]
380 GGGGCCT
HapII 8 CCGG C^CGG
270 CCGG
Hin1I 67 GGCGCC G[GA]^CG[CT]C
220 GGCGTC
HpaII 8 CCGG C^CGG
270 CCGG
HphI 81 GGTGA GGTGA(8/7)
242 TCACC
MseI 428 TTAA T^TAA
548 TTAA
MslI 51 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
141 CACTTTTGTG
MspA1I 53 CAGCGG C[AC]G^C[GT]G
518 CCGCTG
MspI 8 CCGG C^CGG
270 CCGG
RsaI 108 GTAC GT^AC
489 GTAC
Tru9I 428 TTAA T^TAA
548 TTAA
Tsp45I 319 GTGAC ^GT[GC]AC
539 GTGAC
TspRI 423 CACTG CA[GC]TG[ACGT][ACGT]^
537 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccII AciI BsiYI BslI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII FokI HaeIII
HgaI HhaI HinP1I MaeII MaeIII
MboI MnlI MvaI MvnI NdeII
NlaIV Sau3AI Sau96I ScrFI