################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AvrII BalI BbvCI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseRI BsgI BsiHKAI BsiWI BsmI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII CpoI DraI DraIII Eco81I EcoRI EcoRV EcoT14I EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp509I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccIII 52 TCCGGA T^CCGGA AhdI 982 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 494 AGCGCT AGC^GCT AspEI 982 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 916 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1047 CCCGGG C^[CT]CG[GA]G AvaII 110 GGACC G^G[AT]CC AviII 331 TGCGCA TGC^GCA BamHI 154 GGATCC G^GATCC BanII 607 GAGCCC G[GA]GC[CT]^C BbeI 1339 GGCGCC GGCGC^C BbrPI 43 CACGTG CAC^GTG BbsI 12 GAAGAC GAAGAC(2/6) BcgI 209 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 305 TGATCA T^GATCA BglI 409 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 12 GAAGAC GAAGAC(2/6) BsaAI 43 CACGTG [CT]AC^GT[GA] BsaWI 52 TCCGGA [AT]^CCGG[AT] BseAI 52 TCCGGA T^CCGGA BsmBI 1190 GAGACG CGTCTC(1/5) BsoBI 1047 CCCGGG C^[CT]CG[GA]G Bsp1407I 758 TGTACA T^GTACA BspDI 268 ATCGAT AT^CGAT BspEI 52 TCCGGA T^CCGGA BsrBI 1332 GAGCGG CCGCTC(-3/-3) BsrGI 758 TGTACA T^GTACA BssSI 886 CACGAG CACGAG(-5/-1) BstAPI 405 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1226 CCATGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 154 GGATCC [GA]^GATC[CT] ClaI 268 ATCGAT AT^CGAT DraII 689 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 502 GACGAGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 982 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1329 GAAGAG CTCTTC(1/4) Eco47III 494 AGCGCT AGC^GCT EcoNI 681 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 689 AGGCCCT [GA]G^G[ACGT]CC[CT] FbaI 305 TGATCA T^GATCA FspI 331 TGCGCA TGC^GCA KasI 1339 GGCGCC G^GCGCC Ksp632I 1329 GAAGAG CTCTTC(1/4) MflI 154 GGATCC [GA]^GATC[CT] MroI 52 TCCGGA T^CCGGA MspA1I 745 CCGCTG C[AC]G^C[GT]G NarI 1339 GGCGCC GG^CGCC PflFI 916 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 43 CACGTG CAC^GTG PmlI 43 CACGTG CAC^GTG PstI 748 CTGCAG CTGCA^G SapI 1329 GAAGAGC GCTCTTC(1/4) ScaI 251 AGTACT AGT^ACT SfoI 1339 GGCGCC GGC^GCC SmaI 1047 CCCGGG CCC^GGG SphI 405 GCATGC GCATG^C SspBI 758 TGTACA T^GTACA Tth111I 916 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1199 CCATCGTGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 154 GGATCC [GA]^GATC[CT] XmaCI 1047 CCCGGG C^CCGGG XmaI 1047 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcyI 1306 GGCGTC G[GA]^CG[CT]C 1339 GGCGCC AlwI 155 GATCC GGATC(4/5) 154 GGATC BsaHI 1306 GGCGTC G[GA]^CG[CT]C 1339 GGCGCC BsaI 241 GAGACC GGTCTC(1/5) 802 GAGACC BsiEI 817 CGGCCG CG[GA][CT]^CG 1263 CGGCCG BsrI 844 CCAGT ACTGG(1/-1) 1248 CCAGT BstEII 831 GGTGACC G^GT[ACGT]ACC 1350 GGTCACC BstPI 831 GGTGACC G^GT[ACGT]ACC 1350 GGTCACC EagI 817 CGGCCG C^GGCCG 1263 CGGCCG EclXI 817 CGGCCG C^GGCCG 1263 CGGCCG Eco52I 817 CGGCCG C^GGCCG 1263 CGGCCG EcoO65I 831 GGTGACC G^GT[ACGT]ACC 1350 GGTCACC Hin1I 1306 GGCGTC G[GA]^CG[CT]C 1339 GGCGCC HinfI 49 GACTC G^A[ACGT]TC 466 GACTC PleI 49 GACTC GAGTC(4/5) 466 GACTC SfcI 483 CTACAG C^T[GA][CT]AG 748 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AfaI AflIII AluI BanI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I