################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BlnI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 828 CCGC CCGC(-3/-1) AlwNI 1460 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1758 GGGCCC GGGCC^C AspHI 1502 GAGCTC G[AT]GC[AT]^C AvaI 938 CCCGAG C^[CT]CG[GA]G BbvCI 502 CCTCAGC CCTCAGC(-5/-2) BglI 643 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 299 AGATCT A^GATCT BsaWI 1919 ACCGGA [AT]^CCGG[AT] BsgI 1723 CTGCAC GTGCAG(16/14) BsiHKAI 1502 GAGCTC G[AT]GC[AT]^C BsmBI 969 GAGACG CGTCTC(1/5) BsmI 231 GCATTC GAATGC(1/-1) BsoBI 938 CCCGAG C^[CT]CG[GA]G BspHI 584 TCATGA T^CATGA BssSI 1970 CTCGTG CACGAG(-5/-1) BstBI 1643 TTCGAA TT^CGAA BstXI 680 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 299 AGATCT [GA]^GATC[CT] DraI 979 TTTAAA TTT^AAA DrdI 934 GACCCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 962 AGCGCC [GA]GCGC^[CT] HapII 1920 CCGG C^CGG HindIII 1594 AAGCTT A^AGCTT HpaII 1920 CCGG C^CGG MflI 299 AGATCT [GA]^GATC[CT] MspI 1920 CCGG C^CGG NspV 1643 TTCGAA TT^CGAA PflMI 705 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 584 TCATGA T^CATGA SacI 1502 GAGCTC GAGCT^C SapI 361 GAAGAGC GCTCTTC(1/4) SfuI 1643 TTCGAA TT^CGAA SpeI 1893 ACTAGT A^CTAGT SspI 850 AATATT AAT^ATT Van91I 705 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 299 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 375 GATCC GGATC(4/5) 768 GATCC Asp700I 535 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1462 GAAGCTGTTC AvaII 63 GGTCC G^G[AT]CC 2010 GGACC BlpI 477 GCTGAGC GC^T[ACGT]AGC 1015 GCTCAGC Bpu1102I 477 GCTGAGC GC^T[ACGT]AGC 1015 GCTCAGC BsmFI 1169 GGGAC GGGAC(10/14) 2009 GGGAC CelII 477 GCTGAGC GC^T[ACGT]AGC 1015 GCTCAGC DsaI 37 CCATGG C^C[GA][CT]GG 710 CCATGG HgaI 971 GACGC GACGC(5/10) 1785 GCGTC HincII 1282 GTTGAC GT[CT]^[GA]AC 1735 GTCAAC HindII 1282 GTTGAC GT[CT]^[GA]AC 1735 GTCAAC HphI 286 GGTGA GGTGA(8/7) 372 GGTGA MaeII 1496 ACGT A^CGT 1739 ACGT MslI 579 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1726 CACAAGGATG NcoI 37 CCATGG C^CATGG 710 CCATGG NspI 404 GCATGC [GA]CATG^[CT] 830 GCATGC PpuMI 62 GGGTCCT [GA]G^G[AT]CC[CT] 2009 GGGACCT SphI 404 GCATGC GCATG^C 830 GCATGC Tsp45I 91 GTCAC ^GT[GC]AC 248 GTGAC XmnI 535 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1462 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AcsI AfaI AluI ApoI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I