(Circular) (Six-base) MAPSORT of: pFN21AE5082.seq Check: 1683 from: 1 to: 6754 pFN21AE5082 6754 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4097 1937 Size: 2160 4594 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 4817 6201 6469 1632 Size: 1049 2136 1384 268 1917 Fragments arranged by size: 2136 1917 1384 1049 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 5653 6630 1203 Size: 4450 977 1327 Fragments arranged by size: 4450 1327 977 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 5483 5483 Size: 6754 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6754 AlwNI CAG_nnn'CTG Cuts at: 3470 4497 6069 3470 Size: 1027 1572 4155 Fragments arranged by size: 4155 1572 1027 ApaI G_GGCC'C Cuts at: 3767 3767 Size: 6754 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 2987 3005 3435 3652 3782 4063 4289 4441 Size: 1494 18 430 217 130 281 226 152 Cuts at: 4441 6550 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1494 430 281 226 217 152 130 18 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 2943 4079 1870 Size: 73 1000 1136 4545 Fragments arranged by size: 4545 1136 1000 73 BamHI G'GATC_C Cuts at: 4084 4451 4084 Size: 367 6387 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4075 5530 618 Size: 325 217 627 2288 1455 1842 Fragments arranged by size: 2288 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2405 3511 3767 4073 1391 Size: 1014 1106 256 306 4072 Fragments arranged by size: 4072 1106 1014 306 256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2919 3615 928 Size: 971 161 859 696 4067 Fragments arranged by size: 4067 971 859 696 161 BbvCI CC'TCA_GC Cuts at: 2508 2508 Size: 6754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4945 4979 1903 Size: 34 3008 34 3678 Fragments arranged by size: 3678 3008 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4646 5856 4646 Size: 1210 5544 BclI T'GATC_A Cuts at: 1548 1548 Size: 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2654 5365 136 Size: 107 122 71 631 605 982 2711 1525 Fragments arranged by size: 2711 1525 982 631 605 122 107 71 BglII A'GATC_T Cuts at: 2304 6749 2304 Size: 4445 2309 BlpI GC'TnA_GC Cuts at: 2483 3021 4171 2483 Size: 538 1150 5066 Fragments arranged by size: 5066 1150 538 Bme1580I G_kGCm'C Cuts at: 1790 3767 4815 5971 1790 Size: 1977 1048 1156 2573 Fragments arranged by size: 2573 1977 1156 1048 BmgBI CAC'GTC Cuts at: 1318 3502 1318 Size: 2184 4570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4021 4485 5443 Size: 416 53 83 399 24 2604 464 958 Cuts at: 5443 442 Size: 1753 Fragments arranged by size: 2604 1753 958 464 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 3068 3267 3402 5414 1239 Size: 1169 660 199 135 2012 2579 Fragments arranged by size: 2579 2012 1169 660 199 135 Bpu10I CC'TnA_GC Cuts at: 2508 2508 Size: 6754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4879 5744 6042 4879 Size: 865 298 5591 Fragments arranged by size: 5591 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5417 882 Size: 617 3918 2219 Fragments arranged by size: 3918 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4545 493 Size: 711 3341 2702 Fragments arranged by size: 3341 2702 711 BsaBI GATnn'nnATC Cuts at: 6748 6748 Size: 6754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4561 4943 5578 275 Size: 53 83 186 936 3028 382 635 1451 Fragments arranged by size: 3028 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3924 5187 5585 5859 6006 6417 3924 Size: 1263 398 274 147 411 4261 Fragments arranged by size: 4261 1263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6529 Fragments arranged by size: 6529 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 2480 3405 3869 1186 Size: 1183 111 925 464 4071 Fragments arranged by size: 4071 1183 925 464 111 BseYI C'CCAG_C Cuts at: 2649 2964 3270 3400 3664 3695 5957 6453 2649 Size: 315 306 130 264 31 2262 496 2950 Fragments arranged by size: 2950 2262 496 315 306 264 130 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3713 1163 Size: 573 1977 4204 Fragments arranged by size: 4204 1977 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4204 4965 5993 1540 Size: 275 186 2203 761 1028 2301 Fragments arranged by size: 2301 2203 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3511 4815 4900 5971 3511 Size: 1304 85 1071 4294 Fragments arranged by size: 4294 1304 1071 85 BsmI GAATG_Cn' Cuts at: 2236 4265 4358 2236 Size: 2029 93 4632 Fragments arranged by size: 4632 2029 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2968 2968 Size: 6754 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2405 3511 3767 4073 4815 4900 5971 Size: 399 615 1106 256 306 742 85 1071 Cuts at: 5971 1391 Size: 2174 Fragments arranged by size: 2174 1106 1071 742 615 399 306 256 85 BspEI T'CCGG_A Cuts at: 6417 6417 Size: 6754 BspHI T'CATG_A Cuts at: 2589 4643 6373 2589 Size: 2054 1730 2970 Fragments arranged by size: 2970 2054 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4109 844 Size: 3265 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4641 1394 Size: 429 2818 3507 Fragments arranged by size: 3507 2818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2686 2721 2952 3120 5249 5423 65 Size: 1183 1438 35 231 168 2129 174 1396 Fragments arranged by size: 2129 1438 1396 1183 231 174 168 35 BsrFI r'CCGG_y Cuts at: 1700 1810 5398 5585 1700 Size: 110 3588 187 2869 Fragments arranged by size: 3588 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6754 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 3975 4814 5826 3975 Size: 839 1012 4903 Fragments arranged by size: 4903 1012 839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6754 BstBI TT'CG_AA Cuts at: 3649 5558 3649 Size: 1909 4845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6754 BstXI CCAn_nnnn'nTGG Cuts at: 2692 2692 Size: 6754 BstYI r'GATC_y Cuts at: 1972 2304 4084 4451 4836 4853 6305 6391 6403 Size: 332 1780 367 385 17 1452 86 12 Cuts at: 6403 6749 1972 Size: 346 1977 Fragments arranged by size: 1977 1780 1452 385 367 346 332 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2042 2715 513 Size: 552 939 38 673 4552 Fragments arranged by size: 4552 939 673 552 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6015 Fragments arranged by size: 6015 683 56 BtsI GCAGTG_nn' Cuts at: 2257 4270 5064 5084 2257 Size: 2013 794 20 3927 Fragments arranged by size: 3927 2013 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6719 DraI TTT'AAA Cuts at: 2986 4058 2986 Size: 1072 5682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2945 5761 809 Size: 507 1629 2816 1802 Fragments arranged by size: 2816 1802 1629 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719 Size: 54 1425 50 275 18 2371 889 1629 Cuts at: 6719 8 Size: 43 Fragments arranged by size: 2371 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2057 2361 2864 3534 4684 1353 Size: 704 304 503 670 1150 3423 Fragments arranged by size: 3423 1150 704 670 503 304 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3509 3509 Size: 6754 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2681 3068 3267 3402 4817 5414 Size: 393 776 273 387 199 135 1415 597 Cuts at: 5414 6201 6469 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1415 787 776 597 393 387 273 268 199 135 EcoO109I rG'GnC_Cy Cuts at: 2068 3763 3764 4015 2068 Size: 1695 1 251 4807 Fragments arranged by size: 4807 1695 251 1 EcoRI G'AATT_C Cuts at: 4063 4441 4063 Size: 378 6376 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 2971 5901 1678 Size: 1293 2930 2531 Fragments arranged by size: 2930 2531 1293 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2906 2938 3025 3057 3400 Size: 32 135 32 1334 32 87 32 343 Cuts at: 3400 3432 3842 3874 5395 5427 5469 5501 1373 Size: 32 410 32 1521 32 42 32 2626 Fragments arranged by size: 2626 1521 1334 410 343 135 87 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3289 3742 4098 4344 669 Size: 988 281 1351 453 356 246 3079 Fragments arranged by size: 3079 1351 988 453 356 281 246 HindIII A'AGCT_T Cuts at: 748 3599 748 Size: 2851 3903 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 4353 4353 Size: 6754 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5868 6052 1305 Size: 4563 184 2007 Fragments arranged by size: 4563 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4932 Fragments arranged by size: 4932 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2588 3735 4712 5071 5230 518 Size: 648 1422 1147 977 359 159 2042 Fragments arranged by size: 2042 1422 1147 977 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2482 2624 2653 4170 4847 5995 6240 1606 Size: 876 142 29 1517 677 1148 245 2120 Fragments arranged by size: 2120 1517 1148 876 677 245 142 29 NcoI C'CATG_G Cuts at: 513 1065 2004 2042 2715 513 Size: 552 939 38 673 4552 Fragments arranged by size: 4552 939 673 552 38 NdeI CA'TA_TG Cuts at: 387 387 Size: 6754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 2413 2839 4112 5657 1132 Size: 97 1184 426 1273 1545 2229 Fragments arranged by size: 2229 1545 1273 1184 426 97 PasI CC'CwG_GG Cuts at: 1348 2384 1348 Size: 1036 5718 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 2716 1108 Size: 1608 5146 PfoI T'CCnGG_A Cuts at: 2775 2775 Size: 6754 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 4823 4855 6336 6368 3929 Size: 32 862 32 1481 32 4315 Fragments arranged by size: 4315 1481 862 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 4015 2068 Size: 1947 4807 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 4324 4324 Size: 6754 PspOMI G'GGCC_C Cuts at: 3763 3763 Size: 6754 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3630 3662 3630 Size: 32 6722 PstI C_TGCA'G Cuts at: 830 4106 830 Size: 3276 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2482 2624 2653 2482 Size: 142 29 6583 Fragments arranged by size: 6583 142 29 SacI G_AGCT'C Cuts at: 3511 3511 Size: 6754 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SapI GCTCTTCn'nnn_ Cuts at: 2361 2361 Size: 6754 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SfcI C'TryA_G Cuts at: 826 1045 2796 3056 3960 4102 5237 5918 6109 Size: 219 1751 260 904 142 1135 681 191 Cuts at: 6109 826 Size: 1471 Fragments arranged by size: 1751 1471 1135 904 681 260 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4858 5759 6021 820 Size: 197 926 2915 901 262 1553 Fragments arranged by size: 2915 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 3898 152 Size: 3746 3008 SphI G_CATG'C Cuts at: 1132 2413 2839 4112 1132 Size: 1281 426 1273 3774 Fragments arranged by size: 3774 1281 1273 426 SspI AAT'ATT Cuts at: 5 52 2857 4678 5 Size: 47 2805 1821 2081 Fragments arranged by size: 2805 2081 1821 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6754 StyI C'CwwG_G Cuts at: 513 1065 2004 2042 2715 3768 4193 513 Size: 552 939 38 673 1053 425 3074 Fragments arranged by size: 3074 1053 939 673 552 425 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4781 4798 4951 5136 5581 325 Size: 1255 3201 17 153 185 445 1498 Fragments arranged by size: 3201 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3895 5000 6712 Size: 274 80 33 51 1443 1918 1105 1712 Cuts at: 6712 96 Size: 138 Fragments arranged by size: 1918 1712 1443 1105 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2757 2789 6714 6746 2757 Size: 32 3925 32 2765 Fragments arranged by size: 3925 2765 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6754 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 2544 3471 4883 2544 Size: 927 1412 4415 Fragments arranged by size: 4415 1412 927 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI