(Linear) (Six-base) MAPSORT of: KIEE5082.seq Check: 2916 from: 1 to: 2049 KIEE5082.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 676 2049 Size: 676 1373 AlwNI CAG_nnn'CTG Cuts at: 0 1465 2049 Size: 1465 584 ApaI G_GGCC'C Cuts at: 0 1762 2049 Size: 1762 287 ApoI r'AATT_y Cuts at: 0 982 1000 1430 1647 1777 2049 Size: 982 18 430 217 130 272 Fragments arranged by size: 982 430 272 217 130 18 AvaI C'yCGr_G Cuts at: 0 938 2049 Size: 938 1111 BanII G_rGCy'C Cuts at: 0 400 1506 1762 2049 Size: 400 1106 256 287 Fragments arranged by size: 1106 400 287 256 BbsI GAAGACnn'nnnn_ Cuts at: 0 55 914 1610 2049 Size: 55 859 696 439 Fragments arranged by size: 859 696 439 55 BbvCI CC'TCA_GC Cuts at: 0 503 2049 Size: 503 1546 BglI GCCn_nnn'nGGC Cuts at: 0 649 2049 Size: 649 1400 BglII A'GATC_T Cuts at: 0 299 2049 Size: 299 1750 BlpI GC'TnA_GC Cuts at: 0 478 1016 2049 Size: 478 538 1033 Fragments arranged by size: 1033 538 478 Bme1580I G_kGCm'C Cuts at: 0 1762 2049 Size: 1762 287 BmgBI CAC'GTC Cuts at: 0 1497 2049 Size: 1497 552 BmrI ACTGGGnnnn_n' Cuts at: 0 2016 2049 Size: 2016 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 1063 1262 1397 2049 Size: 403 660 199 135 652 Fragments arranged by size: 660 652 403 199 135 Bpu10I CC'TnA_GC Cuts at: 0 503 2049 Size: 503 1546 BsaWI w'CCGG_w Cuts at: 0 1919 2049 Size: 1919 130 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 364 475 1400 1864 2049 Size: 364 111 925 464 185 Fragments arranged by size: 925 464 364 185 111 BseYI C'CCAG_C Cuts at: 0 644 959 1265 1395 1659 1690 2049 Size: 644 315 306 130 264 31 359 Fragments arranged by size: 644 359 315 306 264 130 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1708 2049 Size: 1708 341 BsiHKAI G_wGCw'C Cuts at: 0 1506 2049 Size: 1506 543 BsmI GAATG_Cn' Cuts at: 0 231 2049 Size: 231 1818 BsmBI CGTCTCn'nnnn_ Cuts at: 0 963 2049 Size: 963 1086 Bsp1286I G_dGCh'C Cuts at: 0 400 1506 1762 2049 Size: 400 1106 256 287 Fragments arranged by size: 1106 400 287 256 BspHI T'CATG_A Cuts at: 0 584 2049 Size: 584 1465 BsrDI GCAATG_nn' Cuts at: 0 681 716 947 1115 2049 Size: 681 35 231 168 934 Fragments arranged by size: 934 681 231 168 35 BssSI C'ACGA_G Cuts at: 0 1970 2049 Size: 1970 79 BstBI TT'CG_AA Cuts at: 0 1644 2049 Size: 1644 405 BstXI CCAn_nnnn'nTGG Cuts at: 0 687 2049 Size: 687 1362 BstYI r'GATC_y Cuts at: 0 299 2049 Size: 299 1750 BtgI C'CryG_G Cuts at: 0 37 710 2049 Size: 37 673 1339 Fragments arranged by size: 1339 673 37 BtsI GCAGTG_nn' Cuts at: 0 252 2049 Size: 252 1797 DraI TTT'AAA Cuts at: 0 981 2049 Size: 981 1068 DrdI GACnn_nn'nnGTC Cuts at: 0 940 2049 Size: 940 1109 EarI CTCTTCn'nnn_ Cuts at: 0 52 356 859 1529 2049 Size: 52 304 503 670 520 Fragments arranged by size: 670 520 503 304 52 EcoICRI GAG'CTC Cuts at: 0 1504 2049 Size: 1504 545 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 676 1063 1262 1397 2049 Size: 403 273 387 199 135 652 Fragments arranged by size: 652 403 387 273 199 135 EcoO109I rG'GnC_Cy Cuts at: 0 63 1758 1759 2010 2049 Size: 63 1695 1 251 39 Fragments arranged by size: 1695 251 63 39 1 HaeII r_GCGC'y Cuts at: 0 966 2049 Size: 966 1083 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 901 933 1020 1052 1395 1427 1837 1869 Size: 901 32 87 32 343 32 410 32 Cuts at: 1869 2049 Size: 180 Fragments arranged by size: 901 410 343 180 87 32 32 32 32 HincII GTy'rAC Cuts at: 0 1284 1737 2049 Size: 1284 453 312 Fragments arranged by size: 1284 453 312 HindIII A'AGCT_T Cuts at: 0 1594 2049 Size: 1594 455 MslI CAynn'nnrTG Cuts at: 0 583 1730 2049 Size: 583 1147 319 Fragments arranged by size: 1147 583 319 MspA1I CmG'CkG Cuts at: 0 477 619 648 2049 Size: 477 142 29 1401 Fragments arranged by size: 1401 477 142 29 NcoI C'CATG_G Cuts at: 0 37 710 2049 Size: 37 673 1339 Fragments arranged by size: 1339 673 37 NspI r_CATG'y Cuts at: 0 408 834 2049 Size: 408 426 1215 Fragments arranged by size: 1215 426 408 PasI CC'CwG_GG Cuts at: 0 379 2049 Size: 379 1670 PflMI CCAn_nnn'nTGG Cuts at: 0 711 2049 Size: 711 1338 PfoI T'CCnGG_A Cuts at: 0 770 2049 Size: 770 1279 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1924 1956 2049 Size: 1924 32 93 Fragments arranged by size: 1924 93 32 PpuMI rG'GwC_Cy Cuts at: 0 63 2010 2049 Size: 63 1947 39 Fragments arranged by size: 1947 63 39 PspOMI G'GGCC_C Cuts at: 0 1758 2049 Size: 1758 291 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1625 1657 2049 Size: 1625 32 392 Fragments arranged by size: 1625 392 32 PvuII CAG'CTG Cuts at: 0 477 619 648 2049 Size: 477 142 29 1401 Fragments arranged by size: 1401 477 142 29 SacI G_AGCT'C Cuts at: 0 1506 2049 Size: 1506 543 SapI GCTCTTCn'nnn_ Cuts at: 0 356 2049 Size: 356 1693 SfcI C'TryA_G Cuts at: 0 791 1051 1955 2049 Size: 791 260 904 94 Fragments arranged by size: 904 791 260 94 SpeI A'CTAG_T Cuts at: 0 1893 2049 Size: 1893 156 SphI G_CATG'C Cuts at: 0 408 834 2049 Size: 408 426 1215 Fragments arranged by size: 1215 426 408 SspI AAT'ATT Cuts at: 0 852 2049 Size: 852 1197 StyI C'CwwG_G Cuts at: 0 37 710 1763 2049 Size: 37 673 1053 286 Fragments arranged by size: 1053 673 286 37 TatI w'GTAC_w Cuts at: 0 1890 2049 Size: 1890 159 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 752 784 2049 Size: 752 32 1265 Fragments arranged by size: 1265 752 32 XmnI GAAnn'nnTTC Cuts at: 0 539 1466 2049 Size: 539 927 583 Fragments arranged by size: 927 583 539 Enzymes that do cut: AcuI AlwNI ApaI ApoI AvaI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BssSI BstBI BstXI BstYI BtgI BtsI DraI DrdI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII MslI MspA1I NcoI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PsrI PvuII SacI SapI SfcI SpeI SphI SspI StyI TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BspEI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI