################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BclI BcnI BfrI BglII BlnI BmyI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 433 GCGG CCGC(-3/-1) AlwI 15 GGATC GGATC(4/5) Aor51HI 735 AGCGCT AGC^GCT BcgI 498 GCATCCTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 618 GGATAC GTATCC(6/5) BglI 40 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 49 GCTGAGC GC^T[ACGT]AGC BpmI 177 CTGGAG CTGGAG(16/14) Bpu1102I 49 GCTGAGC GC^T[ACGT]AGC BsaAI 671 TACGTG [CT]AC^GT[GA] BsaJI 350 CCAAGG C^C[ACGT][ACGT]GG BseRI 221 GAGGAG GAGGAG(10/8) BsmAI 268 GAGAC GTCTC(1/5) BsmFI 377 GGGAC GGGAC(10/14) BspMI 575 GCAGGT ACCTGC(4/8) BsrDI 475 GCAATG GCAATG(2/0) BstAPI 397 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 15 GGATCT [GA]^GATC[CT] CelII 49 GCTGAGC GC^T[ACGT]AGC CfoI 736 GCGC GCG^C EaeI 38 TGGCCG [CT]^GGCC[GA] Eco47III 735 AGCGCT AGC^GCT EcoT14I 350 CCAAGG C^C[AT][AT]GG HaeII 735 AGCGCT [GA]GCGC^[CT] HaeIII 39 GGCC GG^CC HhaI 736 GCGC GCG^C HinP1I 736 GCGC G^CGC MfeI 605 CAATTG C^AATTG MflI 15 GGATCT [GA]^GATC[CT] MunI 605 CAATTG C^AATTG NspI 701 ACATGC [GA]CATG^[CT] SbfI 572 CCTGCAGG CCTGCA^GG Sse8387I 572 CCTGCAGG CCTGCA^GG StyI 350 CCAAGG C^C[AT][AT]GG TaqI 9 TCGA T^CGA TfiI 243 GATTC G^A[AT]TC TspRI 339 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 9 TCGA T^CGA XhoII 15 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 400 GAATTT [GA]^AATT[CT] 548 AAATTC AfaI 24 GTAC GT^AC 307 GTAC ApoI 400 GAATTT [GA]^AATT[CT] 548 AAATTC BbsI 226 GAAGAC GAAGAC(2/6) 751 GAAGAC BpuAI 226 GAAGAC GAAGAC(2/6) 751 GAAGAC BssKI 27 CCAGG ^CC[ACGT]GG 176 CCTGG BstNI 27 CCAGG CC^[AT]GG 176 CCTGG DpnII 7 GATC ^GATC 16 GATC EarI 167 GAAGAG CTCTTC(1/4) 718 GAAGAG EcoRII 27 CCAGG ^CC[AT]GG 176 CCTGG HphI 709 GGTGA GGTGA(8/7) 715 GGTGA Ksp632I 167 GAAGAG CTCTTC(1/4) 718 GAAGAG MaeII 672 ACGT A^CGT 746 ACGT MboI 7 GATC ^GATC 16 GATC MseI 283 TTAA T^TAA 554 TTAA MvaI 27 CCAGG CC^[AT]GG 176 CCTGG NdeII 7 GATC ^GATC 16 GATC NlaIII 660 CATG CATG^ 702 CATG PstI 573 CTGCAG CTGCA^G 739 CTGCAG RsaI 24 GTAC GT^AC 307 GTAC SapI 167 GAAGAGC GCTCTTC(1/4) 718 GAAGAGC Sau3AI 7 GATC ^GATC 16 GATC ScrFI 27 CCAGG CC^[ACGT]GG 176 CCTGG SfaNI 601 GATGC GCATC(5/9) 498 GCATC SfcI 573 CTGCAG C^T[GA][CT]AG 739 CTGCAG Tru9I 283 TTAA T^TAA 554 TTAA Tsp45I 293 GTGAC ^GT[GC]AC 710 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AluI BbvI BfaI BsrI BstF5I Cac8I DdeI Fnu4HI FokI HinfI ItaI MaeI MaeIII MboII MnlI MwoI PleI TseI Tsp509I