Restriction map for pFN21AE2194
 (Circular) (Six-base) MAPSORT of: pFN21AE2194.seq  Check: 8324  from: 1  to: 5470



pFN21AE2194  5470 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:55  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3280     278 
   Size:         53      83     186     936    1744    2468

  Fragments arranged by size: 

                2468    1744     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2813    1937 
   Size:        876    4594
 
Acc65I G'GTAC_C

Cuts at:   2791    2791 
   Size:       5470
 
AclI AA'CG_TT

Cuts at:   1563    3597    3970    1563 
   Size:       2034     373    3063

  Fragments arranged by size: 

                3063    2034     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2685    3533    4917    5185    1632 
   Size:       1053     848    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1053     848     268
 
AfeI AGC'GCT

Cuts at:   2742    2742 
   Size:       5470
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5273
 
AflIII A'CryG_T

Cuts at:   1203    4369    5346    1203 
   Size:       3166     977    1327

  Fragments arranged by size: 

                3166    1327     977
 
AgeI A'CCGG_T

Cuts at:   4301    4301 
   Size:       5470
 
AhdI GACnn_n'nnGTC

Cuts at:   4199    4199 
   Size:       5470
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5470
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5470
 
AlwNI CAG_nnn'CTG

Cuts at:   3213    4785    3213 
   Size:       1572    3898
 
ApaLI G'TGCA_C

Cuts at:   3527    4683    3527 
   Size:       1156    4314
 
ApoI r'AATT_y

Cuts at:   1493    2405    2553    2779    3005    3157    5266    1493 
   Size:        912     148     226     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     912     226     226     152     148
 
AseI AT'TA_AT

Cuts at:    160    1032    4024     160 
   Size:        872    2992    1606

  Fragments arranged by size: 

                2992    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5470
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2795    1870 
   Size:         73     852    4545

  Fragments arranged by size: 

                4545     852      73
 
BamHI G'GATC_C

Cuts at:   2800    3167    2800 
   Size:        367    5103
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2791    4246     618 
   Size:        325     217     627    1004    1455    1842

  Fragments arranged by size: 

                1842    1455    1004     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2789    1391 
   Size:       1398    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2238    2763     928 
   Size:        971     339     525    3635

  Fragments arranged by size: 

                3635     971     525     339
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2492    2526    3661    3695    1903 
   Size:         34     555      34    1135      34    3678

  Fragments arranged by size: 

                3678    1135     555      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2617    3362    4572    2617 
   Size:        745    1210    3515

  Fragments arranged by size: 

                3515    1210     745
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5470
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2051    4081     136 
   Size:        107     122      71     631     605     379    2030    1525

  Fragments arranged by size: 

                2030    1525     631     605     379     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5465    5465 
   Size:       5470
 
BlpI GC'TnA_GC

Cuts at:   2055    2887    2055 
   Size:        832    4638
 
Bme1580I G_kGCm'C

Cuts at:   1790    3531    4687    1790 
   Size:       1741    1156    2573

  Fragments arranged by size: 

                2573    1741    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2752    1318 
   Size:       1434    4036
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3201    4159     442 
   Size:        416      53      83     399      24    1784     958    1753

  Fragments arranged by size: 

                1784    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5470
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2203    4130    1239 
   Size:        964    1927    2579

  Fragments arranged by size: 

                2579    1927     964
 
Bpu10I CC'TnA_GC

Cuts at:   2515    2515 
   Size:       5470
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3595    4460    4758    3595 
   Size:        865     298    4307

  Fragments arranged by size: 

                4307     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4133     882 
   Size:        617    2634    2219

  Fragments arranged by size: 

                2634    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2678    3261     493 
   Size:        711    1474     583    2702

  Fragments arranged by size: 

                2702    1474     711     583
 
BsaBI GATnn'nnATC

Cuts at:   5464    5464 
   Size:       5470
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3277    3659    4294     275 
   Size:         53      83     186     936    1744     382     635    1451

  Fragments arranged by size: 

                1744    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3903    4301    4575    4722    5133    3903 
   Size:        398     274     147     411    4240

  Fragments arranged by size: 

                4240     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5245

  Fragments arranged by size: 

                5245     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2241    1186 
   Size:       1055    4415
 
BseYI C'CCAG_C

Cuts at:   4673    5169    4673 
   Size:        496    4974
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4897
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2920    3681    4709    1540 
   Size:        275     186     919     761    1028    2301

  Fragments arranged by size: 

                2301    1028     919     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3531    3616    4687    3531 
   Size:         85    1071    4314

  Fragments arranged by size: 

                4314    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2981    3074    2981 
   Size:         93    5377
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2789    3531    3616    4687    1391 
   Size:        399     999     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     999     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5133    5133 
   Size:       5470
 
BspHI T'CATG_A

Cuts at:   3359    5089    3359 
   Size:       1730    3740
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2571    2825     844 
   Size:       1727     254    3489

  Fragments arranged by size: 

                3489    1727     254
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3357    1394 
   Size:        429    1534    3507

  Fragments arranged by size: 

                3507    1534     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2487    3965    4139      65 
   Size:       1183    1239    1478     174    1396

  Fragments arranged by size: 

                1478    1396    1239    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4114    4301    1700 
   Size:        110    2304     187    2869

  Fragments arranged by size: 

                2869    2304     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5470
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5470
 
BssSI C'ACGA_G

Cuts at:   3530    4542    3530 
   Size:       1012    4458
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2408     794 
   Size:       1614    3856
 
BstBI TT'CG_AA

Cuts at:   4274    4274 
   Size:       5470
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5470
 
BstYI r'GATC_y

Cuts at:   1972    2020    2800    3167    3552    3569    5021    5107    5119 
   Size:         48     780     367     385      17    1452      86      12

Cuts at:   5119    5465    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     780     385     367     346      86      48
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3979

  Fragments arranged by size: 

                3979     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4731

  Fragments arranged by size: 

                4731     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2986    3780    3800    2986 
   Size:        794      20    4656

  Fragments arranged by size: 

                4656     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5470
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5435
 
DraI TTT'AAA

Cuts at:   2774    2774 
   Size:       5470
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4477     809 
   Size:        507    3161    1802

  Fragments arranged by size: 

                3161    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2043    2917    3806 
   Size:         54    1425      50     275      18     213     874     889

Cuts at:   3806    5435       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     874     275     213      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2917    1537 
   Size:        275    1105    4090

  Fragments arranged by size: 

                4090    1105     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2167    2718    3400    1353 
   Size:        814     551     682    3423

  Fragments arranged by size: 

                3423     814     682     551
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4057    4431    4577     387 
   Size:       1064     138    2468     374     146    1280

  Fragments arranged by size: 

                2468    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2203    2685    3533    4130    4917    5185    1239 
   Size:        393     571     482     848     597     787     268    1524

  Fragments arranged by size: 

                1524     848     787     597     571     482     393     268
            
 
EcoRI G'AATT_C

Cuts at:   2779    3157    2779 
   Size:        378    5092
 
FspI TGC'GCA

Cuts at:   3976    3976 
   Size:       5470
 
HaeII r_GCGC'y

Cuts at:   1678    2744    4617    1678 
   Size:       1066    1873    2531

  Fragments arranged by size: 

                2531    1873    1066
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4111    4143    4185    4217    1373 
   Size:         32     135      32    2539      32      42      32    2626

  Fragments arranged by size: 

                2626    2539     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2814    3060     669 
   Size:        988     281     876     246    3079

  Fragments arranged by size: 

                3079     988     876     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5470
 
HpaI GTT'AAC

Cuts at:   3060    3060 
   Size:       5470
 
KpnI G_GTAC'C

Cuts at:   2795    2795 
   Size:       5470
 
MfeI C'AATT_G

Cuts at:   2610    3069    2610 
   Size:        459    5011
 
MluI A'CGCG_T

Cuts at:   5346    5346 
   Size:       5470
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4584    4768    1305 
   Size:       3279     184    2007

  Fragments arranged by size: 

                3279    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3648

  Fragments arranged by size: 

                3648    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3428    3787    3946     518 
   Size:        648    2262     359     159    2042

  Fragments arranged by size: 

                2262    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2886    3563    4711    4956    1606 
   Size:       1280     677    1148     245    2120

  Fragments arranged by size: 

                2120    1280    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3979

  Fragments arranged by size: 

                3979     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5470
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5470
 
NotI GC'GGCC_GC

Cuts at:   2917    2917 
   Size:       5470
 
NspI r_CATG'y

Cuts at:   1132    1229    2710    2828    4373    1132 
   Size:         97    1481     118    1545    2229

  Fragments arranged by size: 

                2229    1545    1481     118      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5470
 
PciI A'CATG_T

Cuts at:   4369    4369 
   Size:       5470
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5470
 
PfoI T'CCnGG_A

Cuts at:   2180    2180 
   Size:       5470
 
PmeI GTTT'AAAC

Cuts at:   2774    2774 
   Size:       5470
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3539    3571    5052    5084    3539 
   Size:         32    1481      32    3925

  Fragments arranged by size: 

                3925    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5470
 
PsiI TTA'TAA

Cuts at:   2370    2460    3040    2370 
   Size:         90     580    4800

  Fragments arranged by size: 

                4800     580      90
 
PstI C_TGCA'G

Cuts at:    830    2582    2748    2822     830 
   Size:       1752     166      74    3478

  Fragments arranged by size: 

                3478    1752     166      74
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5470
 
SalI G'TCGA_C

Cuts at:   1936    2812    1936 
   Size:        876    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2167    2718    2167 
   Size:        551    4919
 
SbfI CC_TGCA'GG

Cuts at:   2582    2822    2582 
   Size:        240    5230
 
ScaI AGT'ACT

Cuts at:   3718    5430    3718 
   Size:       1712    3758
 
SfcI C'TryA_G

Cuts at:    826    1045    2578    2744    2818    3953    4634    4825     826 
   Size:        219    1533     166      74    1135     681     191    1471

  Fragments arranged by size: 

                1533    1471    1135     681     219     191     166      74
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5470
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5470
 
SmaI CCC'GGG

Cuts at:   1872    2797    1872 
   Size:        925    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3574    4475    4737     820 
   Size:        197     926    1631     901     262    1553

  Fragments arranged by size: 

                1631    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3261     493 
   Size:       2768    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5470
 
SphI G_CATG'C

Cuts at:   1132    2828    1132 
   Size:       1696    3774
 
SspI AAT'ATT

Cuts at:      5      52    3394       5 
   Size:         47    3342    2081

  Fragments arranged by size: 

                3342    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5470
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2355    2909     513 
   Size:        552     939     351     554    3074

  Fragments arranged by size: 

                3074     939     554     552     351
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3497    3514    3667    3852    4297     325 
   Size:       1255    1917      17     153     185     445    1498

  Fragments arranged by size: 

                1917    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2311    3716    5428 
   Size:        274      80      33      51    1443     334    1405    1712

Cuts at:   5428      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1405     334     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5430    5462    5430 
   Size:         32    5438
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5470
 
XbaI T'CTAG_A

Cuts at:   2806    2806 
   Size:       5470
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5470
 
XmaI C'CCGG_G

Cuts at:   1870    2795    1870 
   Size:        925    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3599    3599 
   Size:       5470
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3278     276 
   Size:         53      83     186     936    1744    2468

  Fragments arranged by size: 

                2468    1744     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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