(Linear) (Six-base) MAPSORT of: KIEE6309.seq Check: 2626 from: 1 to: 1116 KIEE6309.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1001 1116 Size: 1001 115 AgeI A'CCGG_T Cuts at: 0 85 1116 Size: 85 1031 AlwNI CAG_nnn'CTG Cuts at: 0 272 1026 1116 Size: 272 754 90 Fragments arranged by size: 754 272 90 ApaLI G'TGCA_C Cuts at: 0 1075 1116 Size: 1075 41 ApoI r'AATT_y Cuts at: 0 344 847 1116 Size: 344 503 269 Fragments arranged by size: 503 344 269 AseI AT'TA_AT Cuts at: 0 290 1116 Size: 290 826 AvaI C'yCGr_G Cuts at: 0 32 1040 1116 Size: 32 1008 76 Fragments arranged by size: 1008 76 32 BanII G_rGCy'C Cuts at: 0 40 330 1094 1116 Size: 40 290 764 22 Fragments arranged by size: 764 290 40 22 BglII A'GATC_T Cuts at: 0 311 1116 Size: 311 805 Bme1580I G_kGCm'C Cuts at: 0 1079 1116 Size: 1079 37 BmgBI CAC'GTC Cuts at: 0 339 1086 1116 Size: 339 747 30 Fragments arranged by size: 747 339 30 BmrI ACTGGGnnnn_n' Cuts at: 0 833 1116 Size: 833 283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1116 Size: 151 965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 946 1116 Size: 946 170 BsaI GGTCTCn'nnnn_ Cuts at: 0 499 925 1116 Size: 499 426 191 Fragments arranged by size: 499 426 191 BsaAI yAC'GTr Cuts at: 0 948 1116 Size: 948 168 BsaWI w'CCGG_w Cuts at: 0 85 1116 Size: 85 1031 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 760 790 1116 Size: 760 30 326 Fragments arranged by size: 760 326 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 656 1116 Size: 656 460 BseYI C'CCAG_C Cuts at: 0 25 1116 Size: 25 1091 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 990 1116 Size: 505 485 126 Fragments arranged by size: 505 485 126 BsiHKAI G_wGCw'C Cuts at: 0 1079 1094 1116 Size: 1079 15 22 Fragments arranged by size: 1079 22 15 BsmI GAATG_Cn' Cuts at: 0 611 1116 Size: 611 505 Bsp1286I G_dGCh'C Cuts at: 0 40 330 1079 1094 1116 Size: 40 290 749 15 22 Fragments arranged by size: 749 290 40 22 15 BspHI T'CATG_A Cuts at: 0 624 1094 1116 Size: 624 470 22 Fragments arranged by size: 624 470 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 280 1116 Size: 280 836 BsrFI r'CCGG_y Cuts at: 0 12 85 671 934 971 1116 Size: 12 73 586 263 37 145 Fragments arranged by size: 586 263 145 73 37 12 BstEII G'GTnAC_C Cuts at: 0 527 1116 Size: 527 589 BstXI CCAn_nnnn'nTGG Cuts at: 0 72 1116 Size: 72 1044 BstYI r'GATC_y Cuts at: 0 311 957 1116 Size: 311 646 159 Fragments arranged by size: 646 311 159 BtgI C'CryG_G Cuts at: 0 150 1116 Size: 150 966 BtsI GCAGTG_nn' Cuts at: 0 368 608 1116 Size: 368 240 508 Fragments arranged by size: 508 368 240 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 965 1000 1116 Size: 965 35 116 Fragments arranged by size: 965 116 35 EaeI y'GGCC_r Cuts at: 0 973 1116 Size: 973 143 EarI CTCTTCn'nnn_ Cuts at: 0 622 633 1116 Size: 622 11 483 Fragments arranged by size: 622 483 11 EcoICRI GAG'CTC Cuts at: 0 1092 1116 Size: 1092 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1001 1116 Size: 151 850 115 Fragments arranged by size: 850 151 115 HaeII r_GCGC'y Cuts at: 0 32 79 1116 Size: 32 47 1037 Fragments arranged by size: 1037 47 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 942 974 1116 Size: 942 32 142 Fragments arranged by size: 942 142 32 HincII GTy'rAC Cuts at: 0 381 1116 Size: 381 735 NcoI C'CATG_G Cuts at: 0 150 1116 Size: 150 966 PflMI CCAn_nnn'nTGG Cuts at: 0 890 1116 Size: 890 226 PfoI T'CCnGG_A Cuts at: 0 842 1116 Size: 842 274 PmlI CAC'GTG Cuts at: 0 948 1116 Size: 948 168 PstI C_TGCA'G Cuts at: 0 998 1116 Size: 998 118 SacI G_AGCT'C Cuts at: 0 1094 1116 Size: 1094 22 ScaI AGT'ACT Cuts at: 0 967 1116 Size: 967 149 SfcI C'TryA_G Cuts at: 0 994 1116 Size: 994 122 SmaI CCC'GGG Cuts at: 0 34 1042 1116 Size: 34 1008 74 Fragments arranged by size: 1008 74 34 SmlI C'TyrA_G Cuts at: 0 961 1116 Size: 961 155 StyI C'CwwG_G Cuts at: 0 150 321 693 755 1116 Size: 150 171 372 62 361 Fragments arranged by size: 372 361 171 150 62 TatI w'GTAC_w Cuts at: 0 365 965 1116 Size: 365 600 151 Fragments arranged by size: 600 365 151 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 760 792 1116 Size: 760 32 324 Fragments arranged by size: 760 324 32 XbaI T'CTAG_A Cuts at: 0 405 1116 Size: 405 711 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 762 1116 Size: 762 354 XmaI C'CCGG_G Cuts at: 0 32 1040 1116 Size: 32 1008 76 Fragments arranged by size: 1008 76 32 XmnI GAAnn'nnTTC Cuts at: 0 862 1116 Size: 862 254 Enzymes that do cut: AcuI AgeI AlwNI ApaLI ApoI AseI AvaI BanII BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BstEII BstXI BstYI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI HaeII Hin4I HincII NcoI PflMI PfoI PmlI PstI SacI ScaI SfcI SmaI SmlI StyI TatI TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmtI BplI Bpu10I BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XhoI ZraI