Restriction map for
pFN21AE4309
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MaeII MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 847 GTAC GT^AC
AspHI 103 GAGCAC G[AT]GC[AT]^C
AvaI 516 CCCGAG C^[CT]CG[GA]G
BciVI 127 GGATAC GTATCC(6/5)
BmyI 103 GAGCAC G[AGT]GC[ACT]^C
BpmI 813 CTCCAG CTGGAG(16/14)
BsaBI 142 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 821 GAGACC GGTCTC(1/5)
BsaJI 517 CCGAGG C^C[ACGT][ACGT]GG
BsiHKAI 103 GAGCAC G[AT]GC[AT]^C
BsiYI 906 CCAGAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 906 CCAGAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 151 CGTCTC CGTCTC(1/5)
BsoBI 516 CCCGAG C^[CT]CG[GA]G
Bsp1286I 103 GAGCAC G[AGT]GC[ACT]^C
BsrDI 629 GCAATG GCAATG(2/0)
BssKI 222 CCTGG ^CC[ACGT]GG
BstF5I 765 CATCC GGATG(2/0)
BstNI 222 CCTGG CC^[AT]GG
BstYI 775 GGATCT [GA]^GATC[CT]
EarI 883 CTCTTC CTCTTC(1/4)
EcoRII 222 CCTGG ^CC[AT]GG
FauI 797 CCCGC CCCGC(4/6)
FokI 765 CATCC GGATG(9/13)
HgaI 879 GACGC GACGC(5/10)
HphI 838 GGTGA GGTGA(8/7)
Ksp632I 883 CTCTTC CTCTTC(1/4)
MamI 142 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 775 GGATCT [GA]^GATC[CT]
MspA1I 8 CAGCGG C[AC]G^C[GT]G
MvaI 222 CCTGG CC^[AT]GG
PflMI 906 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 522 GACTC GAGTC(4/5)
PstI 23 CTGCAG CTGCA^G
RsaI 847 GTAC GT^AC
SapI 882 GCTCTTC GCTCTTC(1/4)
ScrFI 222 CCTGG CC^[ACGT]GG
SfaNI 607 GCATC GCATC(5/9)
SfcI 23 CTGCAG C^T[GA][CT]AG
SphI 500 GCATGC GCATG^C
SwaI 644 ATTTAAAT ATTT^AAAT
TaqI 324 TCGA T^CGA
TfiI 267 GATTC G^A[AT]TC
TthHB8I 324 TCGA T^CGA
Van91I 906 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 775 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcsI 399 AAATTT [GA]^AATT[CT]
718 GAATTT
AlwI 354 GATCC GGATC(4/5)
775 GGATC
ApoI 399 AAATTT [GA]^AATT[CT]
718 GAATTT
AvaII 46 GGTCC G^G[AT]CC
277 GGACC
BsmAI 821 GAGAC GTCTC(1/5)
152 GTCTC
BsmFI 445 GGGAC GGGAC(10/14)
877 GGGAC
BspHI 306 TCATGA T^CATGA
558 TCATGA
DdeI 457 CTCAG C^T[ACGT]AG
927 CTCAG
DraI 118 TTTAAA TTT^AAA
645 TTTAAA
HaeIII 261 GGCC GG^CC
316 GGCC
HinfI 267 GATTC G^A[ACGT]TC
522 GACTC
NspI 500 GCATGC [GA]CATG^[CT]
863 GCATGT
RcaI 306 TCATGA T^CATGA
558 TCATGA
Tsp45I 491 GTGAC ^GT[GC]AC
839 GTGAC
XcmI 825 CCAGTTGGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
902 CCAGCCAGAACATGG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BsrI Cac8I
Cfr13I DpnII Fnu4HI ItaI MaeIII
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIII Sau3AI Sau96I
Tru9I TseI Tsp509I TspRI