Restriction map for
pFN21AE1062
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII Acc65I AccIII AflII AgeI
Alw44I ApaI ApaLI AscI Asp718I
AspHI AspI AviII BbrPI BfrI
BglI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiHKAI BspDI BspEI BspLU11I
BssHII Bst1107I BstAPI BstZ17I CelII
ClaI CpoI EcoT22I FseI FspI
HindIII KpnI MamI MluI MroI
NheI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PstI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfcI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 3651 GTCTAC GT^[AC][GT]AC
AclI 144 AACGTT AA^CGTT
AflIII 1515 ACGTGT A^C[GA][CT]GT
Aor51HI 2432 AGCGCT AGC^GCT
AseI 1846 ATTAAT AT^TAAT
AsnI 1846 ATTAAT AT^TAAT
AvrII 3210 CCTAGG C^CTAGG
BbvCI 30 CCTCAGC CCTCAGC(-5/-2)
BciVI 180 GGATAC GTATCC(6/5)
BclI 3945 TGATCA T^GATCA
BglII 3462 AGATCT A^GATCT
BlnI 3210 CCTAGG C^CTAGG
BsiWI 2298 CGTACG C^GTACG
BsmBI 3926 CGTCTC CGTCTC(1/5)
Bsp1407I 1583 TGTACA T^GTACA
BsrGI 1583 TGTACA T^GTACA
BssSI 2267 CTCGTG CACGAG(-5/-1)
BstEII 2805 GGTGACC G^GT[ACGT]ACC
BstPI 2805 GGTGACC G^GT[ACGT]ACC
DrdI 2127 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 95 CGGCCG C^GGCCG
EclXI 95 CGGCCG C^GGCCG
Eco47III 2432 AGCGCT AGC^GCT
Eco52I 95 CGGCCG C^GGCCG
EcoNI 364 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2805 GGTGACC G^GT[ACGT]ACC
FbaI 3945 TGATCA T^GATCA
HpaI 3075 GTTAAC GTT^AAC
KspI 92 CCGCGG CCGC^GG
MfeI 983 CAATTG C^AATTG
MunI 983 CAATTG C^AATTG
NdeI 1700 CATATG CA^TATG
NotI 94 GCGGCCGC GC^GGCCGC
PshAI 3134 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1846 ATTAAT AT^TAAT
Psp1406I 144 AACGTT AA^CGTT
SacII 92 CCGCGG CCGC^GG
SmaI 3819 CCCGGG CCC^GGG
SspBI 1583 TGTACA T^GTACA
XmaCI 3819 CCCGGG C^CCGGG
XmaI 3819 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 93 CGCG CG^CG
114 CGCG
BalI 389 TGGCCA TGG^CCA
3986 TGGCCA
BcgI 3394 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2635 GCAGACAACTCG
BsgI 3939 CTGCAC GTGCAG(16/14)
4243 GTGCAG
BsiEI 95 CGGCCG CG[GA][CT]^CG
1577 CGACCG
BstUI 93 CGCG CG^CG
114 CGCG
DraI 276 TTTAAA TTT^AAA
2769 TTTAAA
EcoRV 735 GATATC GAT^ATC
3565 GATATC
HgaI 299 GACGC GACGC(5/10)
3317 GCGTC
HincII 3075 GTTAAC GT[CT]^[GA]AC
3577 GTTGAC
HindII 3075 GTTAAC GT[CT]^[GA]AC
3577 GTTGAC
MluNI 389 TGGCCA TGG^CCA
3986 TGGCCA
MscI 389 TGGCCA TGG^CCA
3986 TGGCCA
MvnI 93 CGCG CG^CG
114 CGCG
NaeI 3197 GCCGGC GCC^GGC
4202 GCCGGC
NgoMIV 3197 GCCGGC G^CCGGC
4202 GCCGGC
NspI 3647 GCATGT [GA]CATG^[CT]
3934 ACATGC
PflMI 836 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1744 CCAGTGTCTGG
PvuII 2131 CAGCTG CAG^CTG
2140 CAGCTG
SapI 4056 GAAGAGC GCTCTTC(1/4)
200 GCTCTTC
SmlI 992 CTCAAG C^T[CT][GA]AG
3560 CTCAAG
StuI 2084 AGGCCT AGG^CCT
2542 AGGCCT
Van91I 836 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1744 CCAGTGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
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AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApoI
Asp700I AspEI AvaI AvaII BamHI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstBI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspV PleI PpuMI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfoI SfuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI